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Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

No MeSH data available.


Related in: MedlinePlus

c-Fos immunohistochemistry in the cingulate cortex of the sham-operated (sham; A) and myocardial infarction (MI) rats at 3 (B), 7 (C), 14 (D), 28 (E) and 56 days (F) after MI.In the sham group, c-Fos immunoreactivity was hardly detected in the cingulate cortex. In the MI groups, the number of c-Fos-positive (c-Fos+) cells in the cingulate cortex (arrows) was increased at 3 d after MI, peaked at 14 d after MI, and returned to the level of the sham group at 56 d after MI. Scale bar: 100 μm. (G) The mean number of c-Fos+ cells in the cingulate cortex (n = 7 rats per group); *P < 0.05, vs. sham group. One-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
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Figure 4: c-Fos immunohistochemistry in the cingulate cortex of the sham-operated (sham; A) and myocardial infarction (MI) rats at 3 (B), 7 (C), 14 (D), 28 (E) and 56 days (F) after MI.In the sham group, c-Fos immunoreactivity was hardly detected in the cingulate cortex. In the MI groups, the number of c-Fos-positive (c-Fos+) cells in the cingulate cortex (arrows) was increased at 3 d after MI, peaked at 14 d after MI, and returned to the level of the sham group at 56 d after MI. Scale bar: 100 μm. (G) The mean number of c-Fos+ cells in the cingulate cortex (n = 7 rats per group); *P < 0.05, vs. sham group. One-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).

Mentions: c-Fos immunoreactivity was barely detected in the cingulate cortex (Figure 4A) and the piriform cortex in the sham-operated group (Figure 5A). c-Fos-immunoreactive cells in these areas were observed from 3 days after MI, continuously increased in number, peaked in the cingulate cortex at 14 days after MI and in the piriform cortex 7 days after MI; the mean number of c-Fos-immunoreactive cells was 61.8 ± 6.1/mm2 per section from the cingulate cortex (Figure 4B–D and G) and 34.2 ± 4.4/mm2 per section of the piriform cortex (Figure 5B, C and G). Thereafter, the numbers of c-Fos-immunoreactive cells in both the cingulate cortex and the piriform cortex were decreased with time after MI, and few c-Fos-immunoreactive cells were observed in both cortices at 56 days after MI (Figure 4E–G and 5D–G).


Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

c-Fos immunohistochemistry in the cingulate cortex of the sham-operated (sham; A) and myocardial infarction (MI) rats at 3 (B), 7 (C), 14 (D), 28 (E) and 56 days (F) after MI.In the sham group, c-Fos immunoreactivity was hardly detected in the cingulate cortex. In the MI groups, the number of c-Fos-positive (c-Fos+) cells in the cingulate cortex (arrows) was increased at 3 d after MI, peaked at 14 d after MI, and returned to the level of the sham group at 56 d after MI. Scale bar: 100 μm. (G) The mean number of c-Fos+ cells in the cingulate cortex (n = 7 rats per group); *P < 0.05, vs. sham group. One-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590237&req=5

Figure 4: c-Fos immunohistochemistry in the cingulate cortex of the sham-operated (sham; A) and myocardial infarction (MI) rats at 3 (B), 7 (C), 14 (D), 28 (E) and 56 days (F) after MI.In the sham group, c-Fos immunoreactivity was hardly detected in the cingulate cortex. In the MI groups, the number of c-Fos-positive (c-Fos+) cells in the cingulate cortex (arrows) was increased at 3 d after MI, peaked at 14 d after MI, and returned to the level of the sham group at 56 d after MI. Scale bar: 100 μm. (G) The mean number of c-Fos+ cells in the cingulate cortex (n = 7 rats per group); *P < 0.05, vs. sham group. One-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
Mentions: c-Fos immunoreactivity was barely detected in the cingulate cortex (Figure 4A) and the piriform cortex in the sham-operated group (Figure 5A). c-Fos-immunoreactive cells in these areas were observed from 3 days after MI, continuously increased in number, peaked in the cingulate cortex at 14 days after MI and in the piriform cortex 7 days after MI; the mean number of c-Fos-immunoreactive cells was 61.8 ± 6.1/mm2 per section from the cingulate cortex (Figure 4B–D and G) and 34.2 ± 4.4/mm2 per section of the piriform cortex (Figure 5B, C and G). Thereafter, the numbers of c-Fos-immunoreactive cells in both the cingulate cortex and the piriform cortex were decreased with time after MI, and few c-Fos-immunoreactive cells were observed in both cortices at 56 days after MI (Figure 4E–G and 5D–G).

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

No MeSH data available.


Related in: MedlinePlus