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Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

No MeSH data available.


Related in: MedlinePlus

Cresyl violet (CV) staining (the left panels), NeuN immunohistochemistry (the middle panels) and Fluoro-Jade B (F-J B) histofluorescence staining (the right panels) of the piriform cortex in the sham-operated rats (sham; A–C) and myocardial infarction (MI) rats at 14 (D–F) and 56 days (G–I) after MI.In the MI groups, patterns of CV-, NeuN- and F-J B-positive cells in the piriform cortex are similar to those in the sham group. “I–III” indicate layers of the cingulate cortex. Scale bars: 200 μm in A, D, G and 50 μm in B, C, E, F, H and I. d: Days.
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Figure 3: Cresyl violet (CV) staining (the left panels), NeuN immunohistochemistry (the middle panels) and Fluoro-Jade B (F-J B) histofluorescence staining (the right panels) of the piriform cortex in the sham-operated rats (sham; A–C) and myocardial infarction (MI) rats at 14 (D–F) and 56 days (G–I) after MI.In the MI groups, patterns of CV-, NeuN- and F-J B-positive cells in the piriform cortex are similar to those in the sham group. “I–III” indicate layers of the cingulate cortex. Scale bars: 200 μm in A, D, G and 50 μm in B, C, E, F, H and I. d: Days.

Mentions: No neurodegeneration was found in the cingulate cortex (Figure 2A–C) and the piriform cortex (Figure 3A – C) in the sham-operated group, as determined by CV staining, NeuN immunohistochemistry and F-J B histofluorescence staining. The staining pattern in the rat cingulate and piriform cortices in the MI groups was not different from that in the sham-operated group (Figures 2D–I, 3D–I).


Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

Cresyl violet (CV) staining (the left panels), NeuN immunohistochemistry (the middle panels) and Fluoro-Jade B (F-J B) histofluorescence staining (the right panels) of the piriform cortex in the sham-operated rats (sham; A–C) and myocardial infarction (MI) rats at 14 (D–F) and 56 days (G–I) after MI.In the MI groups, patterns of CV-, NeuN- and F-J B-positive cells in the piriform cortex are similar to those in the sham group. “I–III” indicate layers of the cingulate cortex. Scale bars: 200 μm in A, D, G and 50 μm in B, C, E, F, H and I. d: Days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590237&req=5

Figure 3: Cresyl violet (CV) staining (the left panels), NeuN immunohistochemistry (the middle panels) and Fluoro-Jade B (F-J B) histofluorescence staining (the right panels) of the piriform cortex in the sham-operated rats (sham; A–C) and myocardial infarction (MI) rats at 14 (D–F) and 56 days (G–I) after MI.In the MI groups, patterns of CV-, NeuN- and F-J B-positive cells in the piriform cortex are similar to those in the sham group. “I–III” indicate layers of the cingulate cortex. Scale bars: 200 μm in A, D, G and 50 μm in B, C, E, F, H and I. d: Days.
Mentions: No neurodegeneration was found in the cingulate cortex (Figure 2A–C) and the piriform cortex (Figure 3A – C) in the sham-operated group, as determined by CV staining, NeuN immunohistochemistry and F-J B histofluorescence staining. The staining pattern in the rat cingulate and piriform cortices in the MI groups was not different from that in the sham-operated group (Figures 2D–I, 3D–I).

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

No MeSH data available.


Related in: MedlinePlus