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Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells.

Gaude N, Bortfeld S, Erban A, Kopka J, Krajinski F - BMC Plant Biol. (2015)

Bottom Line: Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells.Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Gaude@mpimp-golm.mpg.de.

ABSTRACT

Background: The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning.

Results: Here we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.

Conclusions: High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

No MeSH data available.


Polar primary metabolites which accumulate at least 2-fold in arbuscule containing cells of mycorrhizal roots (myc+) compared to cortex cells of non-mycorrhizal roots (myc-) of Medicago truncatula. Proteinogenic amino acids (black bars), polyols (grey bars) and disaccharides (light grey bars) are highlighted (cf., Additional file 2: Table S1). Yet non-identified metabolites are named by an identifier code of the GolmMetabolome Database (http://gmd.mpimp-golm.mpg.de/)
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Fig2: Polar primary metabolites which accumulate at least 2-fold in arbuscule containing cells of mycorrhizal roots (myc+) compared to cortex cells of non-mycorrhizal roots (myc-) of Medicago truncatula. Proteinogenic amino acids (black bars), polyols (grey bars) and disaccharides (light grey bars) are highlighted (cf., Additional file 2: Table S1). Yet non-identified metabolites are named by an identifier code of the GolmMetabolome Database (http://gmd.mpimp-golm.mpg.de/)

Mentions: In total, 13,638 arbuscule containing cells (arb) and 12,560 cortical cells (cor) were isolated and subjected to GC-EI/TOF-MS analysis. GC-MS measurements revealed a clear difference in the metabolite composition between both cell types (Fig. 2). This indicated that GC-EI/TOF-MS-mediated analysis of primary metabolites is feasible in LMD-isolated cells and the applied method is sensitive enough to analyse limited root material.Fig. 2


Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells.

Gaude N, Bortfeld S, Erban A, Kopka J, Krajinski F - BMC Plant Biol. (2015)

Polar primary metabolites which accumulate at least 2-fold in arbuscule containing cells of mycorrhizal roots (myc+) compared to cortex cells of non-mycorrhizal roots (myc-) of Medicago truncatula. Proteinogenic amino acids (black bars), polyols (grey bars) and disaccharides (light grey bars) are highlighted (cf., Additional file 2: Table S1). Yet non-identified metabolites are named by an identifier code of the GolmMetabolome Database (http://gmd.mpimp-golm.mpg.de/)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4590214&req=5

Fig2: Polar primary metabolites which accumulate at least 2-fold in arbuscule containing cells of mycorrhizal roots (myc+) compared to cortex cells of non-mycorrhizal roots (myc-) of Medicago truncatula. Proteinogenic amino acids (black bars), polyols (grey bars) and disaccharides (light grey bars) are highlighted (cf., Additional file 2: Table S1). Yet non-identified metabolites are named by an identifier code of the GolmMetabolome Database (http://gmd.mpimp-golm.mpg.de/)
Mentions: In total, 13,638 arbuscule containing cells (arb) and 12,560 cortical cells (cor) were isolated and subjected to GC-EI/TOF-MS analysis. GC-MS measurements revealed a clear difference in the metabolite composition between both cell types (Fig. 2). This indicated that GC-EI/TOF-MS-mediated analysis of primary metabolites is feasible in LMD-isolated cells and the applied method is sensitive enough to analyse limited root material.Fig. 2

Bottom Line: Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells.Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Gaude@mpimp-golm.mpg.de.

ABSTRACT

Background: The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning.

Results: Here we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.

Conclusions: High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

No MeSH data available.