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Synergistic algicidal effect and mechanism of two diketopiperazines produced by Chryseobacterium sp. strain GLY-1106 on the harmful bloom-forming Microcystis aeruginosa.

Guo X, Liu X, Pan J, Yang H - Sci Rep (2015)

Bottom Line: Compared with individual treatment, combined treatment with cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) significantly enhanced algicidal activity, accelerated the increase in intracellular reactive oxygen species (ROS) levels in M. aeruginosa, and further decreased the activities of antioxidases, effective quantum yield and maximal electron transport rate of M. aeruginosa.The results also showed that the algicidal characteristics of cyclo(4-OH-Pro-Leu) are distinct from those of cyclo(Pro-Leu).These findings suggest that synergism between algicidal compounds might be used as an effective strategy for the future control of Microcystis blooms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial metabolism, and School of Life Science &Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.

ABSTRACT
A potent algicidal bacterium isolated from Lake Taihu, Chryseobacterium sp. strain GLY-1106, produces two algicidal compounds: 1106-A (cyclo(4-OH-Pro-Leu)) and 1106-B (cyclo(Pro-Leu)). Both diketopiperazines showed strong algicidal activities against Microcystis aeruginosa, the dominant bloom-forming cyanobacterium in Lake Taihu. Interestingly, these two algicidal compounds functioned synergistically. Compared with individual treatment, combined treatment with cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) significantly enhanced algicidal activity, accelerated the increase in intracellular reactive oxygen species (ROS) levels in M. aeruginosa, and further decreased the activities of antioxidases, effective quantum yield and maximal electron transport rate of M. aeruginosa. The results also showed that the algicidal characteristics of cyclo(4-OH-Pro-Leu) are distinct from those of cyclo(Pro-Leu). Cyclo(4-OH-Pro-Leu) mainly interrupted the flux of electron transport in the cyanobacterial photosynthetic system, whereas cyclo(Pro-Leu) mainly inhibited the activity of cyanobacterial intracellular antioxidases. A possible algicidal mechanism for the synergism between cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) is proposed, which is in accordance with their distinct algicidal characteristics in individual and combined treatment. These findings suggest that synergism between algicidal compounds might be used as an effective strategy for the future control of Microcystis blooms.

No MeSH data available.


Related in: MedlinePlus

Molecular structures of algicidal compounds produced by Chryseobacterium sp. strain GLY-1106.
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f1: Molecular structures of algicidal compounds produced by Chryseobacterium sp. strain GLY-1106.

Mentions: In summary, 1106-A and 1106-B were identified as 7-hydroxy-3-isobutyl- hexahydro-pyrrolo[1,2-a]pyrazine-1,4-dione (abbreviated as “cyclo(4-OH-Pro-Leu)”) (Fig. 1a) and hexahydro-3-(2-methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione (“cyclo(Pro-Leu)”) (Fig. 1b), respectively.


Synergistic algicidal effect and mechanism of two diketopiperazines produced by Chryseobacterium sp. strain GLY-1106 on the harmful bloom-forming Microcystis aeruginosa.

Guo X, Liu X, Pan J, Yang H - Sci Rep (2015)

Molecular structures of algicidal compounds produced by Chryseobacterium sp. strain GLY-1106.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589682&req=5

f1: Molecular structures of algicidal compounds produced by Chryseobacterium sp. strain GLY-1106.
Mentions: In summary, 1106-A and 1106-B were identified as 7-hydroxy-3-isobutyl- hexahydro-pyrrolo[1,2-a]pyrazine-1,4-dione (abbreviated as “cyclo(4-OH-Pro-Leu)”) (Fig. 1a) and hexahydro-3-(2-methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione (“cyclo(Pro-Leu)”) (Fig. 1b), respectively.

Bottom Line: Compared with individual treatment, combined treatment with cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) significantly enhanced algicidal activity, accelerated the increase in intracellular reactive oxygen species (ROS) levels in M. aeruginosa, and further decreased the activities of antioxidases, effective quantum yield and maximal electron transport rate of M. aeruginosa.The results also showed that the algicidal characteristics of cyclo(4-OH-Pro-Leu) are distinct from those of cyclo(Pro-Leu).These findings suggest that synergism between algicidal compounds might be used as an effective strategy for the future control of Microcystis blooms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial metabolism, and School of Life Science &Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.

ABSTRACT
A potent algicidal bacterium isolated from Lake Taihu, Chryseobacterium sp. strain GLY-1106, produces two algicidal compounds: 1106-A (cyclo(4-OH-Pro-Leu)) and 1106-B (cyclo(Pro-Leu)). Both diketopiperazines showed strong algicidal activities against Microcystis aeruginosa, the dominant bloom-forming cyanobacterium in Lake Taihu. Interestingly, these two algicidal compounds functioned synergistically. Compared with individual treatment, combined treatment with cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) significantly enhanced algicidal activity, accelerated the increase in intracellular reactive oxygen species (ROS) levels in M. aeruginosa, and further decreased the activities of antioxidases, effective quantum yield and maximal electron transport rate of M. aeruginosa. The results also showed that the algicidal characteristics of cyclo(4-OH-Pro-Leu) are distinct from those of cyclo(Pro-Leu). Cyclo(4-OH-Pro-Leu) mainly interrupted the flux of electron transport in the cyanobacterial photosynthetic system, whereas cyclo(Pro-Leu) mainly inhibited the activity of cyanobacterial intracellular antioxidases. A possible algicidal mechanism for the synergism between cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) is proposed, which is in accordance with their distinct algicidal characteristics in individual and combined treatment. These findings suggest that synergism between algicidal compounds might be used as an effective strategy for the future control of Microcystis blooms.

No MeSH data available.


Related in: MedlinePlus