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Effect of the Human Amniotic Membrane on Liver Regeneration in Rats.

Sipahi M, Şahin S, Arslan E, Börekci H, Metin B, Cantürk NZ - HPB Surg (2015)

Bottom Line: The human amniotic membrane has regenerative effects.Conclusion.However, mesenchymal stem cell research has the potential to accelerate liver regeneration investigations.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, School of Medicine, Bozok University, 66100 Yozgat, Turkey.

ABSTRACT
Introduction. Operations are performed for broader liver surgery indications for a better understanding of hepatic anatomy/physiology and developments in operation technology. Surgery can cure some patients with liver metastasis of some tumors. Nevertheless, postoperative liver failure is the most feared complication causing mortality in patients who have undergone excision of a large liver mass. The human amniotic membrane has regenerative effects. Thus, we investigated the effects of the human amniotic membrane on regeneration of the resected liver. Methods. Twenty female Wistar albino rats were divided into control and experimental groups and underwent a 70% hepatectomy. The human amniotic membrane was placed over the residual liver in the experimental group. Relative liver weight, histopathological features, and biochemical parameters were assessed on postoperative day 3. Results. Total protein and albumin levels were significantly lower in the experimental group than in the control group. No difference in relative liver weight was observed between the groups. Hepatocyte mitotic count was significantly higher in the experimental group than in the control group. Hepatic steatosis was detected in the experimental group. Conclusion. Applying the amniotic membrane to residual liver adversely affected liver regeneration. However, mesenchymal stem cell research has the potential to accelerate liver regeneration investigations.

No MeSH data available.


Related in: MedlinePlus

(a) Microscopic photo illustrating high Ki-67 positivity of a case of control group (Avidin-biotin-peroxidase method, ×200). (b) Microscopic photo illustrating high Ki-67 positivity of an amniotic membrane used case (Avidin-biotin-peroxidase method, ×200).
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fig2: (a) Microscopic photo illustrating high Ki-67 positivity of a case of control group (Avidin-biotin-peroxidase method, ×200). (b) Microscopic photo illustrating high Ki-67 positivity of an amniotic membrane used case (Avidin-biotin-peroxidase method, ×200).

Mentions: The control group had normal appearance of the resected livers. But, in the study group, they were like fatty liver disease (Figure 1). The mean resected liver weight was 5.41 ± 0.36 g in the experimental group and 5.82 ± 0.43 g in the control group. Excision percentages were calculated according to the preoperative liver weight. Consequently, a mean of 64.83% of the liver tissue was excised in the experimental group and 66.76% in the control group (P = 0.103). The results of the biochemical analysis are shown in Table 1. Serum total protein and albumin levels were higher in the control group than in the experimental group (P < 0.01). No significant differences were observed in the AST, ALT, LDH, and total and direct bilirubin levels between the groups. The mean Ki-67 level in the postoperative remnant liver tissue was 54.67 ± 8.99 in the experimental group and 51.75 ± 9.00 in the control group (P = 0.515) (Figure 2). Relative liver weight tended to be higher in the experimental group (29.61 ± 5.60 g) than in the control group (27.34 ± 3.77 g) (P = 0.348). In contrast, the mitotic index in the remnant liver tissue was 4.22 ± 2.91 in the experimental group and 0.50 ± 1.07 in the control group (P < 0.01) (Table 2, Figure 3). Histopathologically, there was no specific change, tumor cell, and significant difference in inflammatory cells in the liver of study and control groups. However, macrovesicular steatosis with large and small droplets was detected in all postoperative remnant liver tissues. Steatosis comprised 70% to 95% of those tissues. Thus, severe hepatic macrovesicular steatosis was observed in residual liver tissue from the experimental group compared with the control group (Figure 4).


Effect of the Human Amniotic Membrane on Liver Regeneration in Rats.

Sipahi M, Şahin S, Arslan E, Börekci H, Metin B, Cantürk NZ - HPB Surg (2015)

(a) Microscopic photo illustrating high Ki-67 positivity of a case of control group (Avidin-biotin-peroxidase method, ×200). (b) Microscopic photo illustrating high Ki-67 positivity of an amniotic membrane used case (Avidin-biotin-peroxidase method, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4589631&req=5

fig2: (a) Microscopic photo illustrating high Ki-67 positivity of a case of control group (Avidin-biotin-peroxidase method, ×200). (b) Microscopic photo illustrating high Ki-67 positivity of an amniotic membrane used case (Avidin-biotin-peroxidase method, ×200).
Mentions: The control group had normal appearance of the resected livers. But, in the study group, they were like fatty liver disease (Figure 1). The mean resected liver weight was 5.41 ± 0.36 g in the experimental group and 5.82 ± 0.43 g in the control group. Excision percentages were calculated according to the preoperative liver weight. Consequently, a mean of 64.83% of the liver tissue was excised in the experimental group and 66.76% in the control group (P = 0.103). The results of the biochemical analysis are shown in Table 1. Serum total protein and albumin levels were higher in the control group than in the experimental group (P < 0.01). No significant differences were observed in the AST, ALT, LDH, and total and direct bilirubin levels between the groups. The mean Ki-67 level in the postoperative remnant liver tissue was 54.67 ± 8.99 in the experimental group and 51.75 ± 9.00 in the control group (P = 0.515) (Figure 2). Relative liver weight tended to be higher in the experimental group (29.61 ± 5.60 g) than in the control group (27.34 ± 3.77 g) (P = 0.348). In contrast, the mitotic index in the remnant liver tissue was 4.22 ± 2.91 in the experimental group and 0.50 ± 1.07 in the control group (P < 0.01) (Table 2, Figure 3). Histopathologically, there was no specific change, tumor cell, and significant difference in inflammatory cells in the liver of study and control groups. However, macrovesicular steatosis with large and small droplets was detected in all postoperative remnant liver tissues. Steatosis comprised 70% to 95% of those tissues. Thus, severe hepatic macrovesicular steatosis was observed in residual liver tissue from the experimental group compared with the control group (Figure 4).

Bottom Line: The human amniotic membrane has regenerative effects.Conclusion.However, mesenchymal stem cell research has the potential to accelerate liver regeneration investigations.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, School of Medicine, Bozok University, 66100 Yozgat, Turkey.

ABSTRACT
Introduction. Operations are performed for broader liver surgery indications for a better understanding of hepatic anatomy/physiology and developments in operation technology. Surgery can cure some patients with liver metastasis of some tumors. Nevertheless, postoperative liver failure is the most feared complication causing mortality in patients who have undergone excision of a large liver mass. The human amniotic membrane has regenerative effects. Thus, we investigated the effects of the human amniotic membrane on regeneration of the resected liver. Methods. Twenty female Wistar albino rats were divided into control and experimental groups and underwent a 70% hepatectomy. The human amniotic membrane was placed over the residual liver in the experimental group. Relative liver weight, histopathological features, and biochemical parameters were assessed on postoperative day 3. Results. Total protein and albumin levels were significantly lower in the experimental group than in the control group. No difference in relative liver weight was observed between the groups. Hepatocyte mitotic count was significantly higher in the experimental group than in the control group. Hepatic steatosis was detected in the experimental group. Conclusion. Applying the amniotic membrane to residual liver adversely affected liver regeneration. However, mesenchymal stem cell research has the potential to accelerate liver regeneration investigations.

No MeSH data available.


Related in: MedlinePlus