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Cripto-1 Promotes the Epithelial-Mesenchymal Transition in Esophageal Squamous Cell Carcinoma Cells.

Huang C, Chen W, Wang X, Zhao J, Li Q, Fu Z - Evid Based Complement Alternat Med (2015)

Bottom Line: Although considerable diagnostic and therapeutic progress has been made in recent years, the prognosis of EC patients still remains dismal due to high rates of recurrence/metastasis and invasion.Previous studies have demonstrated that Epithelial mesenchymal transition (EMT) is proposed as a critical mechanism for the acquisition of malignant phenotypes by epithelial cells.Several lines of evidence have shown that Cripto-1 plays an important oncogenic role during tumorigenesis by promoting EMT.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

ABSTRACT
Esophageal carcinoma is a major public health problem worldwide and one of the most aggressively malignant neoplasms. Although considerable diagnostic and therapeutic progress has been made in recent years, the prognosis of EC patients still remains dismal due to high rates of recurrence/metastasis and invasion. Previous studies have demonstrated that Epithelial mesenchymal transition (EMT) is proposed as a critical mechanism for the acquisition of malignant phenotypes by epithelial cells. Several lines of evidence have shown that Cripto-1 plays an important oncogenic role during tumorigenesis by promoting EMT. The aim of our study was to evaluate the significance of Cripto-1 which plays a role in EMT and its metastasis in esophageal carcinoma. Data of this study suggest that Cripto-1 overexpression is connected with the tumorigenesis and progression of esophageal carcinoma; shRNA might be feasible for the inhibition of the invasion and metastasis of esophageal carcinoma.

No MeSH data available.


Related in: MedlinePlus

Posttransfection mRNA expression of E-cadherin, N-cadherin, and Vimentin by RT-PCR. (a) RT-PCR findings and (b) relative mRNA expression in the mock control, negative control, and cripto-1 interference groups.
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fig8: Posttransfection mRNA expression of E-cadherin, N-cadherin, and Vimentin by RT-PCR. (a) RT-PCR findings and (b) relative mRNA expression in the mock control, negative control, and cripto-1 interference groups.

Mentions: After plasmid transfection of the Eca109 human ESCC cell line, Western blotting revealed that CR-1 interference significantly reduced N-cad and Vim protein expression, while significantly increasing E-cad expression, relative to the mock and negative control groups (P < 0.01, Figure 7). There was no significant differences between the two control groups (P > 0.05). FQ-PCR revealed that the mRNA expression profiles of all three proteins were completely consistent with the Western blot findings (P < 0.01), and there was no significant differences between the two control groups (P > 0.05, Figure 8).


Cripto-1 Promotes the Epithelial-Mesenchymal Transition in Esophageal Squamous Cell Carcinoma Cells.

Huang C, Chen W, Wang X, Zhao J, Li Q, Fu Z - Evid Based Complement Alternat Med (2015)

Posttransfection mRNA expression of E-cadherin, N-cadherin, and Vimentin by RT-PCR. (a) RT-PCR findings and (b) relative mRNA expression in the mock control, negative control, and cripto-1 interference groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4589627&req=5

fig8: Posttransfection mRNA expression of E-cadherin, N-cadherin, and Vimentin by RT-PCR. (a) RT-PCR findings and (b) relative mRNA expression in the mock control, negative control, and cripto-1 interference groups.
Mentions: After plasmid transfection of the Eca109 human ESCC cell line, Western blotting revealed that CR-1 interference significantly reduced N-cad and Vim protein expression, while significantly increasing E-cad expression, relative to the mock and negative control groups (P < 0.01, Figure 7). There was no significant differences between the two control groups (P > 0.05). FQ-PCR revealed that the mRNA expression profiles of all three proteins were completely consistent with the Western blot findings (P < 0.01), and there was no significant differences between the two control groups (P > 0.05, Figure 8).

Bottom Line: Although considerable diagnostic and therapeutic progress has been made in recent years, the prognosis of EC patients still remains dismal due to high rates of recurrence/metastasis and invasion.Previous studies have demonstrated that Epithelial mesenchymal transition (EMT) is proposed as a critical mechanism for the acquisition of malignant phenotypes by epithelial cells.Several lines of evidence have shown that Cripto-1 plays an important oncogenic role during tumorigenesis by promoting EMT.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

ABSTRACT
Esophageal carcinoma is a major public health problem worldwide and one of the most aggressively malignant neoplasms. Although considerable diagnostic and therapeutic progress has been made in recent years, the prognosis of EC patients still remains dismal due to high rates of recurrence/metastasis and invasion. Previous studies have demonstrated that Epithelial mesenchymal transition (EMT) is proposed as a critical mechanism for the acquisition of malignant phenotypes by epithelial cells. Several lines of evidence have shown that Cripto-1 plays an important oncogenic role during tumorigenesis by promoting EMT. The aim of our study was to evaluate the significance of Cripto-1 which plays a role in EMT and its metastasis in esophageal carcinoma. Data of this study suggest that Cripto-1 overexpression is connected with the tumorigenesis and progression of esophageal carcinoma; shRNA might be feasible for the inhibition of the invasion and metastasis of esophageal carcinoma.

No MeSH data available.


Related in: MedlinePlus