Limits...
Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS.

Huang ZH, Wang ZL, Shi BL, Wei D, Chen JX, Wang SL, Gao BJ - Int J Anal Chem (2015)

Bottom Line: Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately.The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times.Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.

View Article: PubMed Central - PubMed

Affiliation: Agricultural University of Hebei, Baoding 071001, China ; Hebei North University, Zhangjiakou 075000, China.

ABSTRACT
Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.

No MeSH data available.


Related in: MedlinePlus

MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution (b–f).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4589620&req=5

fig2: MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution (b–f).

Mentions: DB-5 (30.0 m × 320 μm × 0.25 μm) and HP-5 (30.0 m × 320 μm × 1 μm) capillary columns were tested to separate the target compounds. It was found that there were large differences on the separation efficiency in two capillary columns. Using HP-5 column, the peak of salicylic acid and methyl salicylate overlapped partly, and the separation degree of jasmonic acid and methyl jasmonate was not very good too, whereas, in DB-5 column, jasmonic acid and methyl jasmonate can be separated better and salicylic acid and methyl salicylate can also be completely separated. Based on these results, DB-5 column was used in further studies. Full scan was performed from 40 to 500 amu to determine the retention time and all major ions of each induced compound, and the results are given in Table 3. In the selected ion monitoring (SIM) mode, only one compound was scanned during each scan period to reduce the possibility of interference. MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution were shown in Figure 2. The typical chromatogram of samples was shown in Figure 3.


Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS.

Huang ZH, Wang ZL, Shi BL, Wei D, Chen JX, Wang SL, Gao BJ - Int J Anal Chem (2015)

MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution (b–f).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4589620&req=5

fig2: MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution (b–f).
Mentions: DB-5 (30.0 m × 320 μm × 0.25 μm) and HP-5 (30.0 m × 320 μm × 1 μm) capillary columns were tested to separate the target compounds. It was found that there were large differences on the separation efficiency in two capillary columns. Using HP-5 column, the peak of salicylic acid and methyl salicylate overlapped partly, and the separation degree of jasmonic acid and methyl jasmonate was not very good too, whereas, in DB-5 column, jasmonic acid and methyl jasmonate can be separated better and salicylic acid and methyl salicylate can also be completely separated. Based on these results, DB-5 column was used in further studies. Full scan was performed from 40 to 500 amu to determine the retention time and all major ions of each induced compound, and the results are given in Table 3. In the selected ion monitoring (SIM) mode, only one compound was scanned during each scan period to reduce the possibility of interference. MS total ion (TIC) for 10 μg/mg signal compounds and internal standard solution (a) and SIM chromatograms obtained from standard solution were shown in Figure 2. The typical chromatogram of samples was shown in Figure 3.

Bottom Line: Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately.The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times.Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.

View Article: PubMed Central - PubMed

Affiliation: Agricultural University of Hebei, Baoding 071001, China ; Hebei North University, Zhangjiakou 075000, China.

ABSTRACT
Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.

No MeSH data available.


Related in: MedlinePlus