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Effect of Nourishing "Yin" Removing "Fire" Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats.

Zeng G, Han X, Yu J, Wang Y, Tian Z - Evid Based Complement Alternat Med (2015)

Bottom Line: The vaginal opening time in CHM group was significantly delayed (P < 0.05).Conclusion.The mechanism by which the nourishing "Yin" removing "Fire" Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.

ABSTRACT
Aim. The present study aims to investigate the effects of nourishing "Yin" removing "Fire" (NYRF) Chinese herbal mixture on puberty onset and hypothalamic mTOR expression in female rats. Materials and Methods. Forty female 20-day-old Sprague-Dawley rats were randomly divided into Chinese herbal mixture (CHM) and normal saline (NS) groups. Rats in CHM and NS were treated with NYRF mixture and normal saline, respectively, from d22. Rats in each group were sacrificed on d28, d31, and d34. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and estradiol (E2) levels were analyzed by ELISA. Hypothalamic mTOR mRNA expression levels were determined by RT-PCR and hypothalamic p-mTOR protein levels were assayed by western blot. Results. The vaginal opening time in CHM group was significantly delayed (P < 0.05). On d31, in comparison with NS group, the coefficients of uteri and ovaries, levels of serum LH and E2, and the expression levels of hypothalamic mTOR mRNA and p-mTOR protein were significantly lower in CHM group (P < 0.05). Conclusion. The mechanism by which the nourishing "Yin" removing "Fire" Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.

No MeSH data available.


Effect of the Chinese herbal mixture on the vaginal opening time. By d32, vaginas of 60% rats in NS and only 20% in CHM had opened. By d34, vaginas of 100% rats in NS and 80% in CHM had opened. The average VO time in CHM was significantly delayed compared to that in NS. NS: normal saline, CHM: Chinese herbal mixture.
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fig2: Effect of the Chinese herbal mixture on the vaginal opening time. By d32, vaginas of 60% rats in NS and only 20% in CHM had opened. By d34, vaginas of 100% rats in NS and 80% in CHM had opened. The average VO time in CHM was significantly delayed compared to that in NS. NS: normal saline, CHM: Chinese herbal mixture.

Mentions: The vaginal opening (VO) time of 34-day-old rats was observed in two groups (n = 10, Figure 2). By d34, rats in the NS group had all completed VO, while only 8 rats in the CHM group had their vaginas open. The average time of VO was analyzed and the day of VO of rats with unopened vaginas was recorded as 35 days. The mean VO time in CHM (33.5 ± 0.4 d) was significantly delayed in comparison with that in NS (32.1 ± 0.4 d) and the difference was statistically significant (P < 0.05).


Effect of Nourishing "Yin" Removing "Fire" Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats.

Zeng G, Han X, Yu J, Wang Y, Tian Z - Evid Based Complement Alternat Med (2015)

Effect of the Chinese herbal mixture on the vaginal opening time. By d32, vaginas of 60% rats in NS and only 20% in CHM had opened. By d34, vaginas of 100% rats in NS and 80% in CHM had opened. The average VO time in CHM was significantly delayed compared to that in NS. NS: normal saline, CHM: Chinese herbal mixture.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4589583&req=5

fig2: Effect of the Chinese herbal mixture on the vaginal opening time. By d32, vaginas of 60% rats in NS and only 20% in CHM had opened. By d34, vaginas of 100% rats in NS and 80% in CHM had opened. The average VO time in CHM was significantly delayed compared to that in NS. NS: normal saline, CHM: Chinese herbal mixture.
Mentions: The vaginal opening (VO) time of 34-day-old rats was observed in two groups (n = 10, Figure 2). By d34, rats in the NS group had all completed VO, while only 8 rats in the CHM group had their vaginas open. The average time of VO was analyzed and the day of VO of rats with unopened vaginas was recorded as 35 days. The mean VO time in CHM (33.5 ± 0.4 d) was significantly delayed in comparison with that in NS (32.1 ± 0.4 d) and the difference was statistically significant (P < 0.05).

Bottom Line: The vaginal opening time in CHM group was significantly delayed (P < 0.05).Conclusion.The mechanism by which the nourishing "Yin" removing "Fire" Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.

ABSTRACT
Aim. The present study aims to investigate the effects of nourishing "Yin" removing "Fire" (NYRF) Chinese herbal mixture on puberty onset and hypothalamic mTOR expression in female rats. Materials and Methods. Forty female 20-day-old Sprague-Dawley rats were randomly divided into Chinese herbal mixture (CHM) and normal saline (NS) groups. Rats in CHM and NS were treated with NYRF mixture and normal saline, respectively, from d22. Rats in each group were sacrificed on d28, d31, and d34. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and estradiol (E2) levels were analyzed by ELISA. Hypothalamic mTOR mRNA expression levels were determined by RT-PCR and hypothalamic p-mTOR protein levels were assayed by western blot. Results. The vaginal opening time in CHM group was significantly delayed (P < 0.05). On d31, in comparison with NS group, the coefficients of uteri and ovaries, levels of serum LH and E2, and the expression levels of hypothalamic mTOR mRNA and p-mTOR protein were significantly lower in CHM group (P < 0.05). Conclusion. The mechanism by which the nourishing "Yin" removing "Fire" Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.

No MeSH data available.