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Seasonal Differences in Relative Gene Expression of Putative Central Appetite Regulators in Arctic Charr (Salvelinus alpinus) Do Not Reflect Its Annual Feeding Cycle.

Striberny A, Ravuri CS, Jobling M, Jørgensen EH - PLoS ONE (2015)

Bottom Line: Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments.In the telencephalon, LepR was more highly expressed in January and May than in July.These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

View Article: PubMed Central - PubMed

Affiliation: Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, Tromsø, Norway.

ABSTRACT
The highly seasonal anadromous Arctic charr (Salvelinus alpinus) was used to investigate the possible involvement of altered gene expression of brain neuropeptides in seasonal appetite regulation. Pro-opiomelanocortin (POMCA1, POMCA2), Cocaine and amphetamine regulated transcript (CART), Agouti related Peptide (AgRP), Neuropeptide Y (NPY) and Melanocortin Receptor 4 (MC4-R) genes were examined. The function of centrally expressed Leptin (Lep) in fish remains unclear, so Lep (LepA1, LepA2) and Leptin Receptor (LepR) genes were included in the investigation. In a ten months study gene expression was analysed in hypothalamus, mesencephalon and telencephalon of immature charr held under natural photoperiod (69°38'N) and ambient temperature and given excess feed. From April to the beginning of June the charr did not feed and lost weight, during July and August they were feeding and had a marked increase in weight and condition factor, and from November until the end of the study the charr lost appetite and decreased in weight and condition factor. Brain compartments were sampled from non-feeding charr (May), feeding charr (July), and non-feeding charr (January). Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments. The non-feeding charr (May, January) had a lower expression of the anorexigenic LepA1, MC4-R and LepR in hypothalamus and a higher expression of the orexigenic NPY and AgRP in mesencephalon, than the feeding charr (July). In the telencephalon, LepR was more highly expressed in January and May than in July. These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

No MeSH data available.


Related in: MedlinePlus

Relative gene expression in the hypothalamus (A), the mesencephalon (B), and the telencephalon (C) in May 2012 and January 2013.Expression levels in brain compartments sampled in May 2012 and January 2013 are presented relative to those sampled in July 2012 (July data were used as calibrator samples and were set equal to one as indicated by the dashed line). Data are presented as mean (n = 8–10) ± s.e.m.
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pone.0138857.g002: Relative gene expression in the hypothalamus (A), the mesencephalon (B), and the telencephalon (C) in May 2012 and January 2013.Expression levels in brain compartments sampled in May 2012 and January 2013 are presented relative to those sampled in July 2012 (July data were used as calibrator samples and were set equal to one as indicated by the dashed line). Data are presented as mean (n = 8–10) ± s.e.m.

Mentions: In the hypothalamus there were no significant differences in gene expression of either the anorexigenic POMCs, CART and LepA2, or orexigenic AgRP between sampling dates (Fig 2A, Table 3). The expression of NPY was very low (Ct > cycle 35) on all sampling dates and the data were not analysed statistically. In May and January gene expressions of LepA1, MC4-R and LepR were two-fold lower than in July, whereas no significant differences in the expression of these genes were found between the fish sampled in May and January (Fig 2A, Table 3).


Seasonal Differences in Relative Gene Expression of Putative Central Appetite Regulators in Arctic Charr (Salvelinus alpinus) Do Not Reflect Its Annual Feeding Cycle.

Striberny A, Ravuri CS, Jobling M, Jørgensen EH - PLoS ONE (2015)

Relative gene expression in the hypothalamus (A), the mesencephalon (B), and the telencephalon (C) in May 2012 and January 2013.Expression levels in brain compartments sampled in May 2012 and January 2013 are presented relative to those sampled in July 2012 (July data were used as calibrator samples and were set equal to one as indicated by the dashed line). Data are presented as mean (n = 8–10) ± s.e.m.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589418&req=5

pone.0138857.g002: Relative gene expression in the hypothalamus (A), the mesencephalon (B), and the telencephalon (C) in May 2012 and January 2013.Expression levels in brain compartments sampled in May 2012 and January 2013 are presented relative to those sampled in July 2012 (July data were used as calibrator samples and were set equal to one as indicated by the dashed line). Data are presented as mean (n = 8–10) ± s.e.m.
Mentions: In the hypothalamus there were no significant differences in gene expression of either the anorexigenic POMCs, CART and LepA2, or orexigenic AgRP between sampling dates (Fig 2A, Table 3). The expression of NPY was very low (Ct > cycle 35) on all sampling dates and the data were not analysed statistically. In May and January gene expressions of LepA1, MC4-R and LepR were two-fold lower than in July, whereas no significant differences in the expression of these genes were found between the fish sampled in May and January (Fig 2A, Table 3).

Bottom Line: Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments.In the telencephalon, LepR was more highly expressed in January and May than in July.These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

View Article: PubMed Central - PubMed

Affiliation: Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, Tromsø, Norway.

ABSTRACT
The highly seasonal anadromous Arctic charr (Salvelinus alpinus) was used to investigate the possible involvement of altered gene expression of brain neuropeptides in seasonal appetite regulation. Pro-opiomelanocortin (POMCA1, POMCA2), Cocaine and amphetamine regulated transcript (CART), Agouti related Peptide (AgRP), Neuropeptide Y (NPY) and Melanocortin Receptor 4 (MC4-R) genes were examined. The function of centrally expressed Leptin (Lep) in fish remains unclear, so Lep (LepA1, LepA2) and Leptin Receptor (LepR) genes were included in the investigation. In a ten months study gene expression was analysed in hypothalamus, mesencephalon and telencephalon of immature charr held under natural photoperiod (69°38'N) and ambient temperature and given excess feed. From April to the beginning of June the charr did not feed and lost weight, during July and August they were feeding and had a marked increase in weight and condition factor, and from November until the end of the study the charr lost appetite and decreased in weight and condition factor. Brain compartments were sampled from non-feeding charr (May), feeding charr (July), and non-feeding charr (January). Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments. The non-feeding charr (May, January) had a lower expression of the anorexigenic LepA1, MC4-R and LepR in hypothalamus and a higher expression of the orexigenic NPY and AgRP in mesencephalon, than the feeding charr (July). In the telencephalon, LepR was more highly expressed in January and May than in July. These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

No MeSH data available.


Related in: MedlinePlus