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Seasonal Differences in Relative Gene Expression of Putative Central Appetite Regulators in Arctic Charr (Salvelinus alpinus) Do Not Reflect Its Annual Feeding Cycle.

Striberny A, Ravuri CS, Jobling M, Jørgensen EH - PLoS ONE (2015)

Bottom Line: Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments.In the telencephalon, LepR was more highly expressed in January and May than in July.These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

View Article: PubMed Central - PubMed

Affiliation: Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, Tromsø, Norway.

ABSTRACT
The highly seasonal anadromous Arctic charr (Salvelinus alpinus) was used to investigate the possible involvement of altered gene expression of brain neuropeptides in seasonal appetite regulation. Pro-opiomelanocortin (POMCA1, POMCA2), Cocaine and amphetamine regulated transcript (CART), Agouti related Peptide (AgRP), Neuropeptide Y (NPY) and Melanocortin Receptor 4 (MC4-R) genes were examined. The function of centrally expressed Leptin (Lep) in fish remains unclear, so Lep (LepA1, LepA2) and Leptin Receptor (LepR) genes were included in the investigation. In a ten months study gene expression was analysed in hypothalamus, mesencephalon and telencephalon of immature charr held under natural photoperiod (69°38'N) and ambient temperature and given excess feed. From April to the beginning of June the charr did not feed and lost weight, during July and August they were feeding and had a marked increase in weight and condition factor, and from November until the end of the study the charr lost appetite and decreased in weight and condition factor. Brain compartments were sampled from non-feeding charr (May), feeding charr (July), and non-feeding charr (January). Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments. The non-feeding charr (May, January) had a lower expression of the anorexigenic LepA1, MC4-R and LepR in hypothalamus and a higher expression of the orexigenic NPY and AgRP in mesencephalon, than the feeding charr (July). In the telencephalon, LepR was more highly expressed in January and May than in July. These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

No MeSH data available.


Related in: MedlinePlus

Body mass development (A) and Fulton’s Condition Factor K (B) of the fish sampled for mRNA quantification.Black dots: Sampled in May 2012, White dots: Sampled in July 2012, Triangles: Sampled in January 2013. Data are presented as mean (n = 10) ± s.e.m. Dotted line: temperature profile. White area: hours of daylight.
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pone.0138857.g001: Body mass development (A) and Fulton’s Condition Factor K (B) of the fish sampled for mRNA quantification.Black dots: Sampled in May 2012, White dots: Sampled in July 2012, Triangles: Sampled in January 2013. Data are presented as mean (n = 10) ± s.e.m. Dotted line: temperature profile. White area: hours of daylight.

Mentions: The body mass of the fish sampled on May 15 had decreased between March 28 and the sampling date, indicating that the fish were in a prolonged non-feeding state (Fig 1A). For fish sampled on July 27 there had been a marked increase in body weight from mid-June onwards, indicating that the fish were feeding and growing. Fish sampled on January 18 had gradually decreased in body mass since October, indicating an extended period without feeding. The condition factor K (Fig 1B) of the fish sampled in May and January had decreased in the period prior to the samplings, whereas there had been a marked increase in condition factor prior to the sampling of fish in July; this provides confirmation of the feeding status of the sampled fish. All sampled charr were immature and had a GSI < 1% (Table 1).


Seasonal Differences in Relative Gene Expression of Putative Central Appetite Regulators in Arctic Charr (Salvelinus alpinus) Do Not Reflect Its Annual Feeding Cycle.

Striberny A, Ravuri CS, Jobling M, Jørgensen EH - PLoS ONE (2015)

Body mass development (A) and Fulton’s Condition Factor K (B) of the fish sampled for mRNA quantification.Black dots: Sampled in May 2012, White dots: Sampled in July 2012, Triangles: Sampled in January 2013. Data are presented as mean (n = 10) ± s.e.m. Dotted line: temperature profile. White area: hours of daylight.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589418&req=5

pone.0138857.g001: Body mass development (A) and Fulton’s Condition Factor K (B) of the fish sampled for mRNA quantification.Black dots: Sampled in May 2012, White dots: Sampled in July 2012, Triangles: Sampled in January 2013. Data are presented as mean (n = 10) ± s.e.m. Dotted line: temperature profile. White area: hours of daylight.
Mentions: The body mass of the fish sampled on May 15 had decreased between March 28 and the sampling date, indicating that the fish were in a prolonged non-feeding state (Fig 1A). For fish sampled on July 27 there had been a marked increase in body weight from mid-June onwards, indicating that the fish were feeding and growing. Fish sampled on January 18 had gradually decreased in body mass since October, indicating an extended period without feeding. The condition factor K (Fig 1B) of the fish sampled in May and January had decreased in the period prior to the samplings, whereas there had been a marked increase in condition factor prior to the sampling of fish in July; this provides confirmation of the feeding status of the sampled fish. All sampled charr were immature and had a GSI < 1% (Table 1).

Bottom Line: Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments.In the telencephalon, LepR was more highly expressed in January and May than in July.These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

View Article: PubMed Central - PubMed

Affiliation: Department of Arctic and Marine Biology, UiT-The Arctic University of Norway, Tromsø, Norway.

ABSTRACT
The highly seasonal anadromous Arctic charr (Salvelinus alpinus) was used to investigate the possible involvement of altered gene expression of brain neuropeptides in seasonal appetite regulation. Pro-opiomelanocortin (POMCA1, POMCA2), Cocaine and amphetamine regulated transcript (CART), Agouti related Peptide (AgRP), Neuropeptide Y (NPY) and Melanocortin Receptor 4 (MC4-R) genes were examined. The function of centrally expressed Leptin (Lep) in fish remains unclear, so Lep (LepA1, LepA2) and Leptin Receptor (LepR) genes were included in the investigation. In a ten months study gene expression was analysed in hypothalamus, mesencephalon and telencephalon of immature charr held under natural photoperiod (69°38'N) and ambient temperature and given excess feed. From April to the beginning of June the charr did not feed and lost weight, during July and August they were feeding and had a marked increase in weight and condition factor, and from November until the end of the study the charr lost appetite and decreased in weight and condition factor. Brain compartments were sampled from non-feeding charr (May), feeding charr (July), and non-feeding charr (January). Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments. The non-feeding charr (May, January) had a lower expression of the anorexigenic LepA1, MC4-R and LepR in hypothalamus and a higher expression of the orexigenic NPY and AgRP in mesencephalon, than the feeding charr (July). In the telencephalon, LepR was more highly expressed in January and May than in July. These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.

No MeSH data available.


Related in: MedlinePlus