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In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants.

Passari AK, Mishra VK, Gupta VK, Yadav MK, Saikia R, Singh BP - PLoS ONE (2015)

Bottom Line: Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC) and BOX repetitive elements.The two potential strains Streptomyces sp. (BPSAC34) and Leifsonia xyli (BPSAC24) were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L.) under greenhouse conditions.These results clearly suggest the possibility of using endophytic actinomycetes as bioinoculant for plant growth promotion, nutrient mobilization or as biocontrol agent against fungal phytopathogens for sustainable agriculture.

View Article: PubMed Central - PubMed

Affiliation: Molecular Microbiology and Systematic Laboratory, Department of Biotechnology, Aizawl, Mizoram University, Mizoram, 796004, India.

ABSTRACT
Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC) and BOX repetitive elements. Genetic relatedness based on both ERIC and BOX-PCR generates specific patterns corresponding to particular genotypes. Exponentially grown antagonistic isolates were used to evaluate phosphate solubilization, siderophores, HCN, ammonia, chitinase, indole-3-acetic acid production, as well as antifungal activities. Out of 22 isolates, the amount of indole-3-acetic acid (IAA) ranging between 10-32 μg/ml was produced by 20 isolates and all isolates were positive for ammonia production ranging between 5.2 to 54 mg/ml. Among 22 isolates tested, the amount of hydroxamate-type siderophores were produced by 16 isolates ranging between 5.2 to 36.4 μg/ml, while catechols-type siderophores produced by 5 isolates ranging from 3.2 to 5.4 μg/ml. Fourteen isolates showed the solubilisation of inorganic phosphorous ranging from 3.2 to 32.6 mg/100ml. Chitinase and HCN production was shown by 19 and 15 different isolates, respectively. In addition, genes of indole acetic acid (iaaM) and chitinase (chiC) were successively amplified from 20 and 19 isolates respectively. The two potential strains Streptomyces sp. (BPSAC34) and Leifsonia xyli (BPSAC24) were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L.) under greenhouse conditions. This study is the first published report that actinomycetes can be isolated as endophytes from within these plants and were shown to have antagonistic and plant growth promoting abilities. These results clearly suggest the possibility of using endophytic actinomycetes as bioinoculant for plant growth promotion, nutrient mobilization or as biocontrol agent against fungal phytopathogens for sustainable agriculture.

No MeSH data available.


Dendrogram generated from ERIC PCR genomic fingerprints of endophytic actinomycetes isolates using Ntsys 2.0.
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pone.0139468.g004: Dendrogram generated from ERIC PCR genomic fingerprints of endophytic actinomycetes isolates using Ntsys 2.0.

Mentions: All the antagonistic endophytic actinomycetes generated a specific pattern with ERIC-PCR and genetic diversity of isolates was not significant from either location. The fingerprinting pattern yielded discriminatory patterns with 3 to 12 fragments ranging in size from approx. <100 bp to 3.0 kb which demonstrate the usefulness of this technique in differentiate the isolates. Dendrogram generated by ERIC-PCR divided the isolates into four clusters (A-D). Cluster A consist of 10 isolates, belonging to the genus Streptomyces. Isolate Streptomyces 5 (BPSAC5) and Streptomyces 33 (BPSAC33) showed 100% similarity and both identified as Streptomyces sp. based on 16S rRNA gene sequencing. Cluster B consist of four isolates all belonging to the genus Microbacterium. Cluster C consist of five isolates comprising different genera belonging to Leifsonia, Brevibacterium and Streptomyces and the cluster D consists of two isolates both belonging to the genus Streptomyces (Fig 4).


In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants.

Passari AK, Mishra VK, Gupta VK, Yadav MK, Saikia R, Singh BP - PLoS ONE (2015)

Dendrogram generated from ERIC PCR genomic fingerprints of endophytic actinomycetes isolates using Ntsys 2.0.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589368&req=5

pone.0139468.g004: Dendrogram generated from ERIC PCR genomic fingerprints of endophytic actinomycetes isolates using Ntsys 2.0.
Mentions: All the antagonistic endophytic actinomycetes generated a specific pattern with ERIC-PCR and genetic diversity of isolates was not significant from either location. The fingerprinting pattern yielded discriminatory patterns with 3 to 12 fragments ranging in size from approx. <100 bp to 3.0 kb which demonstrate the usefulness of this technique in differentiate the isolates. Dendrogram generated by ERIC-PCR divided the isolates into four clusters (A-D). Cluster A consist of 10 isolates, belonging to the genus Streptomyces. Isolate Streptomyces 5 (BPSAC5) and Streptomyces 33 (BPSAC33) showed 100% similarity and both identified as Streptomyces sp. based on 16S rRNA gene sequencing. Cluster B consist of four isolates all belonging to the genus Microbacterium. Cluster C consist of five isolates comprising different genera belonging to Leifsonia, Brevibacterium and Streptomyces and the cluster D consists of two isolates both belonging to the genus Streptomyces (Fig 4).

Bottom Line: Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC) and BOX repetitive elements.The two potential strains Streptomyces sp. (BPSAC34) and Leifsonia xyli (BPSAC24) were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L.) under greenhouse conditions.These results clearly suggest the possibility of using endophytic actinomycetes as bioinoculant for plant growth promotion, nutrient mobilization or as biocontrol agent against fungal phytopathogens for sustainable agriculture.

View Article: PubMed Central - PubMed

Affiliation: Molecular Microbiology and Systematic Laboratory, Department of Biotechnology, Aizawl, Mizoram University, Mizoram, 796004, India.

ABSTRACT
Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC) and BOX repetitive elements. Genetic relatedness based on both ERIC and BOX-PCR generates specific patterns corresponding to particular genotypes. Exponentially grown antagonistic isolates were used to evaluate phosphate solubilization, siderophores, HCN, ammonia, chitinase, indole-3-acetic acid production, as well as antifungal activities. Out of 22 isolates, the amount of indole-3-acetic acid (IAA) ranging between 10-32 μg/ml was produced by 20 isolates and all isolates were positive for ammonia production ranging between 5.2 to 54 mg/ml. Among 22 isolates tested, the amount of hydroxamate-type siderophores were produced by 16 isolates ranging between 5.2 to 36.4 μg/ml, while catechols-type siderophores produced by 5 isolates ranging from 3.2 to 5.4 μg/ml. Fourteen isolates showed the solubilisation of inorganic phosphorous ranging from 3.2 to 32.6 mg/100ml. Chitinase and HCN production was shown by 19 and 15 different isolates, respectively. In addition, genes of indole acetic acid (iaaM) and chitinase (chiC) were successively amplified from 20 and 19 isolates respectively. The two potential strains Streptomyces sp. (BPSAC34) and Leifsonia xyli (BPSAC24) were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L.) under greenhouse conditions. This study is the first published report that actinomycetes can be isolated as endophytes from within these plants and were shown to have antagonistic and plant growth promoting abilities. These results clearly suggest the possibility of using endophytic actinomycetes as bioinoculant for plant growth promotion, nutrient mobilization or as biocontrol agent against fungal phytopathogens for sustainable agriculture.

No MeSH data available.