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Vitamin A Deficiency Impairs Mucin Expression and Suppresses the Mucosal Immune Function of the Respiratory Tract in Chicks.

Fan X, Liu S, Liu G, Zhao J, Jiao H, Wang X, Song Z, Lin H - PLoS ONE (2015)

Bottom Line: The chicken immune system is immature at the time of hatching.The development of the respiratory immune system after hatching is vital to young chicks.In conclusion, vitamin A deficiency suppressed the immunity of the airway by decreasing the IgA and mucin concentrations in neonatal chicks.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control, Taian, Shandong, 271018, China.

ABSTRACT
The chicken immune system is immature at the time of hatching. The development of the respiratory immune system after hatching is vital to young chicks. The aim of this study was to investigate the effect of dietary vitamin A supplement levels on respiratory mucin and IgA production in chicks. In this study, 120 one-day-old broiler chicks were randomly divided into 4 groups consisting of three replicates of 10 broilers and subjected to dietary vitamin A supplement levels of 0, 1,500, 6,000, or 12,000 IU/kg for seven days. Compared with control birds, vitamin A supplementation significantly increased the mucin and IgA levels in the bronchoalveolar lavage fluid (BALF) as well as the IgA level in serum. In the lungs, vitamin A supplementation downregulated TNF-α and EGFR mRNA expression. The TGF-β and MUC5AC mRNA expression levels were upregulated by vitamin A supplementation at a dose of 6,000 IU/kg, and the IL-13 mRNA expression level was increased at the 12,000 IU/kg supplement level. Vitamin A deficiency (control) significantly decreased the mRNA expression levels of MUC2, IgA, EGFR, IL-13 and TGF-β in trachea tissue. Histological section analysis revealed that the number of goblet cells in the tracheal epithelium was less in the 0 and 12,000 IU/kg vitamin A supplement groups than in the other groups. In conclusion, vitamin A deficiency suppressed the immunity of the airway by decreasing the IgA and mucin concentrations in neonatal chicks. This study suggested that a suitable level of vitamin A is essential for the secretion of IgA and mucin in the respiratory tract by regulating the gene expression of cytokines and epithelial growth factors.

No MeSH data available.


Related in: MedlinePlus

Effect of the dietary vitamin A supplemental levels (0, 1500, 6000, and 12000 IU/kg) on the TNF-α (A), IL-6 (B), IL-13 (C), TGF-α (D), TGF-β (E), and EGFR (F) mRNA expression levels in the trachea and lung tissue.Data are presented as the means ± SEM (n = 9); a, b, c: Means with different letters differ significantly, P < 0.05; *: P < 0.05.
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pone.0139131.g005: Effect of the dietary vitamin A supplemental levels (0, 1500, 6000, and 12000 IU/kg) on the TNF-α (A), IL-6 (B), IL-13 (C), TGF-α (D), TGF-β (E), and EGFR (F) mRNA expression levels in the trachea and lung tissue.Data are presented as the means ± SEM (n = 9); a, b, c: Means with different letters differ significantly, P < 0.05; *: P < 0.05.

Mentions: In the lung, the gene expression level of TNF-03B1 was downregulated by vitamin A supplementation at all three levels (P < 0.05, Fig 5A). Compared with the control, however, the mRNA level of IL-13 tended to be increased by vitamin A supplementation at the dose of 12,000 IU/kg (P = 0.059, Fig 5C). The expression of TGF-β was increased by vitamin A supplementation (P < 0.001), and the TGF-β mRNA level was higher in the 6,000 and 12000 IU/kg vitamin A treatment groups compared to the control group (P < 0.001, Fig 5E). In contrast, the mRNA levels of IL-6 and TGF-α were not changed by vitamin A treatment (P > 0.05, Fig 5B and 5D).


Vitamin A Deficiency Impairs Mucin Expression and Suppresses the Mucosal Immune Function of the Respiratory Tract in Chicks.

Fan X, Liu S, Liu G, Zhao J, Jiao H, Wang X, Song Z, Lin H - PLoS ONE (2015)

Effect of the dietary vitamin A supplemental levels (0, 1500, 6000, and 12000 IU/kg) on the TNF-α (A), IL-6 (B), IL-13 (C), TGF-α (D), TGF-β (E), and EGFR (F) mRNA expression levels in the trachea and lung tissue.Data are presented as the means ± SEM (n = 9); a, b, c: Means with different letters differ significantly, P < 0.05; *: P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589363&req=5

pone.0139131.g005: Effect of the dietary vitamin A supplemental levels (0, 1500, 6000, and 12000 IU/kg) on the TNF-α (A), IL-6 (B), IL-13 (C), TGF-α (D), TGF-β (E), and EGFR (F) mRNA expression levels in the trachea and lung tissue.Data are presented as the means ± SEM (n = 9); a, b, c: Means with different letters differ significantly, P < 0.05; *: P < 0.05.
Mentions: In the lung, the gene expression level of TNF-03B1 was downregulated by vitamin A supplementation at all three levels (P < 0.05, Fig 5A). Compared with the control, however, the mRNA level of IL-13 tended to be increased by vitamin A supplementation at the dose of 12,000 IU/kg (P = 0.059, Fig 5C). The expression of TGF-β was increased by vitamin A supplementation (P < 0.001), and the TGF-β mRNA level was higher in the 6,000 and 12000 IU/kg vitamin A treatment groups compared to the control group (P < 0.001, Fig 5E). In contrast, the mRNA levels of IL-6 and TGF-α were not changed by vitamin A treatment (P > 0.05, Fig 5B and 5D).

Bottom Line: The chicken immune system is immature at the time of hatching.The development of the respiratory immune system after hatching is vital to young chicks.In conclusion, vitamin A deficiency suppressed the immunity of the airway by decreasing the IgA and mucin concentrations in neonatal chicks.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control, Taian, Shandong, 271018, China.

ABSTRACT
The chicken immune system is immature at the time of hatching. The development of the respiratory immune system after hatching is vital to young chicks. The aim of this study was to investigate the effect of dietary vitamin A supplement levels on respiratory mucin and IgA production in chicks. In this study, 120 one-day-old broiler chicks were randomly divided into 4 groups consisting of three replicates of 10 broilers and subjected to dietary vitamin A supplement levels of 0, 1,500, 6,000, or 12,000 IU/kg for seven days. Compared with control birds, vitamin A supplementation significantly increased the mucin and IgA levels in the bronchoalveolar lavage fluid (BALF) as well as the IgA level in serum. In the lungs, vitamin A supplementation downregulated TNF-α and EGFR mRNA expression. The TGF-β and MUC5AC mRNA expression levels were upregulated by vitamin A supplementation at a dose of 6,000 IU/kg, and the IL-13 mRNA expression level was increased at the 12,000 IU/kg supplement level. Vitamin A deficiency (control) significantly decreased the mRNA expression levels of MUC2, IgA, EGFR, IL-13 and TGF-β in trachea tissue. Histological section analysis revealed that the number of goblet cells in the tracheal epithelium was less in the 0 and 12,000 IU/kg vitamin A supplement groups than in the other groups. In conclusion, vitamin A deficiency suppressed the immunity of the airway by decreasing the IgA and mucin concentrations in neonatal chicks. This study suggested that a suitable level of vitamin A is essential for the secretion of IgA and mucin in the respiratory tract by regulating the gene expression of cytokines and epithelial growth factors.

No MeSH data available.


Related in: MedlinePlus