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Ammonia Affects Astroglial Proliferation in Culture.

Bodega G, Segura B, Ciordia S, Mena Mdel C, López-Fernández LA, García MI, Trabado I, Suárez I - PLoS ONE (2015)

Bottom Line: The DNA content was reduced in the treated cells, and BrdU incorporation diminished.A reduction in cell viability was also noted.The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.

ABSTRACT
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

No MeSH data available.


Related in: MedlinePlus

Effect of ammonia on astroglial proliferation.Change in astroglial cell number after 10 days of exposure to ammonia. Standard deviations are not shown for 1 and 3 days since differences between the control and ammonia-treated cells were not significant at these times. *p<0.05, **p<0.01.
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pone.0139619.g002: Effect of ammonia on astroglial proliferation.Change in astroglial cell number after 10 days of exposure to ammonia. Standard deviations are not shown for 1 and 3 days since differences between the control and ammonia-treated cells were not significant at these times. *p<0.05, **p<0.01.

Mentions: Exposure to ammonia induced a dose- and time-dependent reduction in cell number. Clear, significant reductions were observed after 10 days in the 3 and 5 mM ammonia treatments (44.8 and 50.4% respectively; p<0.01) (Fig 2). These reductions were not due to an increase in the number of dead cells; their number was small and very similar under all experimental condition (data not shown).


Ammonia Affects Astroglial Proliferation in Culture.

Bodega G, Segura B, Ciordia S, Mena Mdel C, López-Fernández LA, García MI, Trabado I, Suárez I - PLoS ONE (2015)

Effect of ammonia on astroglial proliferation.Change in astroglial cell number after 10 days of exposure to ammonia. Standard deviations are not shown for 1 and 3 days since differences between the control and ammonia-treated cells were not significant at these times. *p<0.05, **p<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589356&req=5

pone.0139619.g002: Effect of ammonia on astroglial proliferation.Change in astroglial cell number after 10 days of exposure to ammonia. Standard deviations are not shown for 1 and 3 days since differences between the control and ammonia-treated cells were not significant at these times. *p<0.05, **p<0.01.
Mentions: Exposure to ammonia induced a dose- and time-dependent reduction in cell number. Clear, significant reductions were observed after 10 days in the 3 and 5 mM ammonia treatments (44.8 and 50.4% respectively; p<0.01) (Fig 2). These reductions were not due to an increase in the number of dead cells; their number was small and very similar under all experimental condition (data not shown).

Bottom Line: The DNA content was reduced in the treated cells, and BrdU incorporation diminished.A reduction in cell viability was also noted.The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.

ABSTRACT
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

No MeSH data available.


Related in: MedlinePlus