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Ammonia Affects Astroglial Proliferation in Culture.

Bodega G, Segura B, Ciordia S, Mena Mdel C, López-Fernández LA, García MI, Trabado I, Suárez I - PLoS ONE (2015)

Bottom Line: The DNA content was reduced in the treated cells, and BrdU incorporation diminished.A reduction in cell viability was also noted.The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.

ABSTRACT
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

No MeSH data available.


Related in: MedlinePlus

Effect of ammonia and serum on astroglial proliferation.Effect of ammonia (NH4Cl) exposure on astroglial proliferation was tested at two FBS concentrations (0.5 and 5%). **p<0.01. The percentage value is calculated taking the value for the 5% FBS control as 100% (1.65 x 105 cells). Columns and bars represent means and SDs.
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pone.0139619.g001: Effect of ammonia and serum on astroglial proliferation.Effect of ammonia (NH4Cl) exposure on astroglial proliferation was tested at two FBS concentrations (0.5 and 5%). **p<0.01. The percentage value is calculated taking the value for the 5% FBS control as 100% (1.65 x 105 cells). Columns and bars represent means and SDs.

Mentions: To determine the optimum conditions for studying changes in the proliferative activity of the cells, a preliminary experiment was performed. It is logical to assume that cells growing under very low FBS content conditions might have a low proliferative rate, and that this might make it difficult to detect any effect of ammonia. Confluent astroglial cells were exposed to 5 mM NH4Cl for 10 days with either 0.5 or 5% FBS. Fig 1 shows that ammonia affects astroglial proliferation, but this effect was only clear for the cells cultured with 5% FBS: a significant 40% reduction (p<0.01) was detected with 5% FBS, but only a non-significant 20% reduction was observed in cells growing with 0.5% FBS.


Ammonia Affects Astroglial Proliferation in Culture.

Bodega G, Segura B, Ciordia S, Mena Mdel C, López-Fernández LA, García MI, Trabado I, Suárez I - PLoS ONE (2015)

Effect of ammonia and serum on astroglial proliferation.Effect of ammonia (NH4Cl) exposure on astroglial proliferation was tested at two FBS concentrations (0.5 and 5%). **p<0.01. The percentage value is calculated taking the value for the 5% FBS control as 100% (1.65 x 105 cells). Columns and bars represent means and SDs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589356&req=5

pone.0139619.g001: Effect of ammonia and serum on astroglial proliferation.Effect of ammonia (NH4Cl) exposure on astroglial proliferation was tested at two FBS concentrations (0.5 and 5%). **p<0.01. The percentage value is calculated taking the value for the 5% FBS control as 100% (1.65 x 105 cells). Columns and bars represent means and SDs.
Mentions: To determine the optimum conditions for studying changes in the proliferative activity of the cells, a preliminary experiment was performed. It is logical to assume that cells growing under very low FBS content conditions might have a low proliferative rate, and that this might make it difficult to detect any effect of ammonia. Confluent astroglial cells were exposed to 5 mM NH4Cl for 10 days with either 0.5 or 5% FBS. Fig 1 shows that ammonia affects astroglial proliferation, but this effect was only clear for the cells cultured with 5% FBS: a significant 40% reduction (p<0.01) was detected with 5% FBS, but only a non-significant 20% reduction was observed in cells growing with 0.5% FBS.

Bottom Line: The DNA content was reduced in the treated cells, and BrdU incorporation diminished.A reduction in cell viability was also noted.The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.

ABSTRACT
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.

No MeSH data available.


Related in: MedlinePlus