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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Cis-regulatory elements in the promoters of the soybean PLC gene family.The values of -900 to -100 represent the upstream region (from translation start site) of the promoter of all the PLC genes. Various elements such as ABRE, MBS, TC-rich, Skn-1 and GCN4 are present in the promoter.
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pone.0138467.g006: Cis-regulatory elements in the promoters of the soybean PLC gene family.The values of -900 to -100 represent the upstream region (from translation start site) of the promoter of all the PLC genes. Various elements such as ABRE, MBS, TC-rich, Skn-1 and GCN4 are present in the promoter.

Mentions: Gene expression can be regulated at several levels, such as transcription and post-translational modification. The promoter is the region of DNA that initiates the transcription of a particular gene. Cis-regulatory elements are functional DNA sequences that precisely control the temporal and spatial expression patterns of the genes expressed in higher plants [49]. In this study, soybean PLC genes were mapped on the genome and their promoter sequences were obtained. The cis-regulatory elements, such as MBS (MYB binding site), ABRE (abscisic acid responsive element), TC-rich, Skn-1 and GCN4, were analyzed. These cis-regulatory elements are known to regulate various stress responses and plant development [50–53]. Each soybean PLC gene had 1–6 cis-regulatory elements in its promoter region (Fig 6). The promoter of GmPLC10, which was highly expressed in all organs and induced strongly by abiotic stresses, contained one TC-rich and Skn-1, and two ABRE motifs. GmPLC3, GmPLC11 and GmPLC12 had more cis-regulatory elements, which might account for their responsiveness to abiotic stresses and development (Fig 6, S3 Table). Surprisingly, six cis-regulatory elements were found in the promoter of GmPLC9, but GmPLC9 transcripts were low in all organs and insensitive to nearly all abiotic stresses. This is in agreement with previous data on Glu-A1-1, -B1-1 and -B1-2 [54]. This result implies that other mechanisms modulate the expression of GmPLC9.


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Cis-regulatory elements in the promoters of the soybean PLC gene family.The values of -900 to -100 represent the upstream region (from translation start site) of the promoter of all the PLC genes. Various elements such as ABRE, MBS, TC-rich, Skn-1 and GCN4 are present in the promoter.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g006: Cis-regulatory elements in the promoters of the soybean PLC gene family.The values of -900 to -100 represent the upstream region (from translation start site) of the promoter of all the PLC genes. Various elements such as ABRE, MBS, TC-rich, Skn-1 and GCN4 are present in the promoter.
Mentions: Gene expression can be regulated at several levels, such as transcription and post-translational modification. The promoter is the region of DNA that initiates the transcription of a particular gene. Cis-regulatory elements are functional DNA sequences that precisely control the temporal and spatial expression patterns of the genes expressed in higher plants [49]. In this study, soybean PLC genes were mapped on the genome and their promoter sequences were obtained. The cis-regulatory elements, such as MBS (MYB binding site), ABRE (abscisic acid responsive element), TC-rich, Skn-1 and GCN4, were analyzed. These cis-regulatory elements are known to regulate various stress responses and plant development [50–53]. Each soybean PLC gene had 1–6 cis-regulatory elements in its promoter region (Fig 6). The promoter of GmPLC10, which was highly expressed in all organs and induced strongly by abiotic stresses, contained one TC-rich and Skn-1, and two ABRE motifs. GmPLC3, GmPLC11 and GmPLC12 had more cis-regulatory elements, which might account for their responsiveness to abiotic stresses and development (Fig 6, S3 Table). Surprisingly, six cis-regulatory elements were found in the promoter of GmPLC9, but GmPLC9 transcripts were low in all organs and insensitive to nearly all abiotic stresses. This is in agreement with previous data on Glu-A1-1, -B1-1 and -B1-2 [54]. This result implies that other mechanisms modulate the expression of GmPLC9.

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.