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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Expression of GmPLCs in various organs.The expression levels of soybean PLCs was compared with EF1A which the expression level of EF1A was defined as “1”. a: expression level > 0.1; b: expression level from 0.01 to 0.1; c: expression level from 0.001 to 0.01; d: expression level < 0.001. Please note that the expression levels of different PLCs are scaled differently.
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pone.0138467.g005: Expression of GmPLCs in various organs.The expression levels of soybean PLCs was compared with EF1A which the expression level of EF1A was defined as “1”. a: expression level > 0.1; b: expression level from 0.01 to 0.1; c: expression level from 0.001 to 0.01; d: expression level < 0.001. Please note that the expression levels of different PLCs are scaled differently.

Mentions: Plant PLC members have been characterized in various cellular processes and signaling networks, and are triggered in response to developmental events in different plant species [47]. To investigate the steady-state expression patterns of the PLC genes in soybean, qRT-PCR was used to detect transcript levels in various organs. High transcript levels of GmPLC7 were detected in all organs, especially stems and early pods. GmPLC12 expression was found in early pods specifically, although low transcript levels were found in other organs (Fig 5). In Arabidopsis, AtPLC2 was highly expressed in leaves, stems, roots and flowers [33]. Recently, microarray analysis of the Arabidopsis PI-PLC gene family was carried out and confirmed that AtPLC2 transcripts were highly expressed in all organs [48]. GmPLC3, GmPLC5, GmPLC10 and GmPLC11 had medium transcript levels in all organs. The expression levels of GmPLC1, GmPLC6, GmPLC8 and GmPLC9 were lower than the others (Fig 5). In rice, OsPLC1 and OsPLC3 were highly expressed in all organs, but lower expression of OsPLC2 was detected in various organs [24]. The varied expression patterns of the soybean PLC gene family imply that GmPLCs are involved in different stages during soybean development.


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Expression of GmPLCs in various organs.The expression levels of soybean PLCs was compared with EF1A which the expression level of EF1A was defined as “1”. a: expression level > 0.1; b: expression level from 0.01 to 0.1; c: expression level from 0.001 to 0.01; d: expression level < 0.001. Please note that the expression levels of different PLCs are scaled differently.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g005: Expression of GmPLCs in various organs.The expression levels of soybean PLCs was compared with EF1A which the expression level of EF1A was defined as “1”. a: expression level > 0.1; b: expression level from 0.01 to 0.1; c: expression level from 0.001 to 0.01; d: expression level < 0.001. Please note that the expression levels of different PLCs are scaled differently.
Mentions: Plant PLC members have been characterized in various cellular processes and signaling networks, and are triggered in response to developmental events in different plant species [47]. To investigate the steady-state expression patterns of the PLC genes in soybean, qRT-PCR was used to detect transcript levels in various organs. High transcript levels of GmPLC7 were detected in all organs, especially stems and early pods. GmPLC12 expression was found in early pods specifically, although low transcript levels were found in other organs (Fig 5). In Arabidopsis, AtPLC2 was highly expressed in leaves, stems, roots and flowers [33]. Recently, microarray analysis of the Arabidopsis PI-PLC gene family was carried out and confirmed that AtPLC2 transcripts were highly expressed in all organs [48]. GmPLC3, GmPLC5, GmPLC10 and GmPLC11 had medium transcript levels in all organs. The expression levels of GmPLC1, GmPLC6, GmPLC8 and GmPLC9 were lower than the others (Fig 5). In rice, OsPLC1 and OsPLC3 were highly expressed in all organs, but lower expression of OsPLC2 was detected in various organs [24]. The varied expression patterns of the soybean PLC gene family imply that GmPLCs are involved in different stages during soybean development.

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.