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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Expression of soybean PLCs in roots under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
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pone.0138467.g004: Expression of soybean PLCs in roots under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.

Mentions: PA reportedly binds to a number of proteins that play a role during water limiting conditions, such as drought and salinity stress, and has been shown to play an important role in maintaining root system architecture [45]. In addition, DAG, which is a product of PLC, can be subsequently phosphorylated to PA by DGK. Therefore, the expression profiles of the soybean PLC genes were also analyzed in roots. The expression of GmPLC1 decreased under salt, saline-alkali, alkali and PEG treatments in roots. GmPLC3, GmPLC5, GmPLC6, GmPLC10, GmPLC11 and GmPLC12 transcripts were induced by salt stress (Fig 4). The expression levels of GmPLC3, GmPLC5, GmPLC6, GmPLC10, GmPLC11 and GmPLC12 were increased under alkali treatment. GmPLC3, GmPLC5, GmPLC6, GmPLC10 and GmPLC11 were expressed under saline-alkaline treatment. ABA treatment stimulated the transcription of GmPLC5, GmPLC6, GmPLC7, GmPLC10 and GmPLC12, especially GmPLC7, GmPLC10 and GmPLC12 (Fig 4). Although the expression profiles of PLCs in roots have not been analyzed before, PLC signaling has been characterized in the activation of plasmolysis in root cells [46]. Moreover, most of the soybean PLC genes were induced by abiotic stress, especially ABA treatment. This suggests that soybean PLC genes are involved in various stress pathways.


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Expression of soybean PLCs in roots under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g004: Expression of soybean PLCs in roots under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
Mentions: PA reportedly binds to a number of proteins that play a role during water limiting conditions, such as drought and salinity stress, and has been shown to play an important role in maintaining root system architecture [45]. In addition, DAG, which is a product of PLC, can be subsequently phosphorylated to PA by DGK. Therefore, the expression profiles of the soybean PLC genes were also analyzed in roots. The expression of GmPLC1 decreased under salt, saline-alkali, alkali and PEG treatments in roots. GmPLC3, GmPLC5, GmPLC6, GmPLC10, GmPLC11 and GmPLC12 transcripts were induced by salt stress (Fig 4). The expression levels of GmPLC3, GmPLC5, GmPLC6, GmPLC10, GmPLC11 and GmPLC12 were increased under alkali treatment. GmPLC3, GmPLC5, GmPLC6, GmPLC10 and GmPLC11 were expressed under saline-alkaline treatment. ABA treatment stimulated the transcription of GmPLC5, GmPLC6, GmPLC7, GmPLC10 and GmPLC12, especially GmPLC7, GmPLC10 and GmPLC12 (Fig 4). Although the expression profiles of PLCs in roots have not been analyzed before, PLC signaling has been characterized in the activation of plasmolysis in root cells [46]. Moreover, most of the soybean PLC genes were induced by abiotic stress, especially ABA treatment. This suggests that soybean PLC genes are involved in various stress pathways.

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.