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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Expression of soybean PLCs in leaves under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
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pone.0138467.g003: Expression of soybean PLCs in leaves under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.

Mentions: To investigate the expression patterns of the soybean PLC genes, qRT-PCR was used to detect their transcript levels in leaves and roots under PEG, ABA, salt, alkali and saline-alkaline treatments. Our result indicated that the expression of GmPLC1, GmPLC2, GmPLC4 in leaves and GmPLC2, GmPLC4, GmPLC8 in roots cannot be detected. In leaves, GmPLC6 transcripts accumulated dramatically under saline-alkali and ABA treatments (Fig 3). In pea leaves, PsPLC transcription was also stimulated after 30 min induction by ABA [40]. It has been reported that PLC plays a role in the events associated with the inhibition of stomatal opening by ABA [41]. This implies that PLC takes part in the regulation of drought stress in plants. On the other hand, the expression levels of GmPLC9 were induced by PEG stress, specifically. Transcript levels of GmPLC10 were increased after alkali treatment. After induction by salt, GmPLC12 transcript levels were more than 3-fold greater than in the control (Fig 3). Recently, TaPLC1 was characterized to have biological functions in regulating seedling growth and responses to drought and salinity stress [42]. The expression of TaPLC1 and AtPLC5 could be induced by salt and PEG treatments [15, 42]. The overexpression of AtPLC9 improved thermotolerance in Arabidopsis [43]. These results show that plant PLCs play a positive role in abiotic stress responses. However, the expression levels of GmPLC3 and GmPLC9 decreased under ABA treatment, and GmPLC6 transcripts were decreased under PEG treatment (Fig 3). This phenomenon was also observed in rice, in which OsPLC4 transcripts decreased during salt, cold and drought stresses [24]. It was also shown that one family member had dominant negative effects on the function of the others in Arabidopsis [44]. It is essential to investigate the mechanism by which PLCs function in the regulation of plant abiotic stress responses.


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Expression of soybean PLCs in leaves under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g003: Expression of soybean PLCs in leaves under abiotic stress.The expression levels of soybean PLCs, which were shown in Y-axis, were compared with the control (0 h). Please note that the expression levels of different PLCs are scaled differently.
Mentions: To investigate the expression patterns of the soybean PLC genes, qRT-PCR was used to detect their transcript levels in leaves and roots under PEG, ABA, salt, alkali and saline-alkaline treatments. Our result indicated that the expression of GmPLC1, GmPLC2, GmPLC4 in leaves and GmPLC2, GmPLC4, GmPLC8 in roots cannot be detected. In leaves, GmPLC6 transcripts accumulated dramatically under saline-alkali and ABA treatments (Fig 3). In pea leaves, PsPLC transcription was also stimulated after 30 min induction by ABA [40]. It has been reported that PLC plays a role in the events associated with the inhibition of stomatal opening by ABA [41]. This implies that PLC takes part in the regulation of drought stress in plants. On the other hand, the expression levels of GmPLC9 were induced by PEG stress, specifically. Transcript levels of GmPLC10 were increased after alkali treatment. After induction by salt, GmPLC12 transcript levels were more than 3-fold greater than in the control (Fig 3). Recently, TaPLC1 was characterized to have biological functions in regulating seedling growth and responses to drought and salinity stress [42]. The expression of TaPLC1 and AtPLC5 could be induced by salt and PEG treatments [15, 42]. The overexpression of AtPLC9 improved thermotolerance in Arabidopsis [43]. These results show that plant PLCs play a positive role in abiotic stress responses. However, the expression levels of GmPLC3 and GmPLC9 decreased under ABA treatment, and GmPLC6 transcripts were decreased under PEG treatment (Fig 3). This phenomenon was also observed in rice, in which OsPLC4 transcripts decreased during salt, cold and drought stresses [24]. It was also shown that one family member had dominant negative effects on the function of the others in Arabidopsis [44]. It is essential to investigate the mechanism by which PLCs function in the regulation of plant abiotic stress responses.

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.