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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Phylogenetic analysis of the PLC family in soybean, Arabidopsis and rice.
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pone.0138467.g002: Phylogenetic analysis of the PLC family in soybean, Arabidopsis and rice.

Mentions: There are two types of phospholipase C (PI-PLC and NPC) in plants. However, no NPCs were found in the soybean genome. Phylogenetic analysis was carried out on the plant PLC and NPC gene families in Arabidopsis, rice and soybean. Soybean PLCs showed high homology to PI-PLCs from Arabidopsis and rice. On the other hand, OsNPCs and AtNPCs shared similarity with each other (Fig 2). The differences between the PLC types derived from diversity in structure and amino acid composition. These results suggest that soybean PLC genes belong to the PLC gene family and should be classified as PI-PLCs.


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Phylogenetic analysis of the PLC family in soybean, Arabidopsis and rice.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g002: Phylogenetic analysis of the PLC family in soybean, Arabidopsis and rice.
Mentions: There are two types of phospholipase C (PI-PLC and NPC) in plants. However, no NPCs were found in the soybean genome. Phylogenetic analysis was carried out on the plant PLC and NPC gene families in Arabidopsis, rice and soybean. Soybean PLCs showed high homology to PI-PLCs from Arabidopsis and rice. On the other hand, OsNPCs and AtNPCs shared similarity with each other (Fig 2). The differences between the PLC types derived from diversity in structure and amino acid composition. These results suggest that soybean PLC genes belong to the PLC gene family and should be classified as PI-PLCs.

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.