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Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.


Domain analysis of soybean PLCs.
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pone.0138467.g001: Domain analysis of soybean PLCs.

Mentions: To characterize the soybean PLC genes, their domain structure was analyzed and a phylogenetic tree was constructed. PLC proteins contain a Pleckstrin Homology (PH) domain, EF hand, catalytic domain and C2 domain in animals. Some also contain a PDZ binding motif, Ras-binding domain, guanine-nucleotide-exchange factor for the Ras domain and Src homology domain [5]. However, in plants, such as soybean, PLCs only contain an EF hand, catalytic domain and C2 domain (Fig 1). The EF hand, which also found in DGK, has a key role in Ca2+ binding [34]. The catalytic domain, which contains X and Y domains, is conserved among eukaryotic PLCs [35], especially soybean PLC (S2 Fig). The C2 domain can bind Ca2+ and other effectors, including phospholipids, inositol phosphates, and proteins [36–38]. The C2 domain appears at the beginning of phospholipase D [39], but at the end of soybean PLC (Fig 1).


Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).

Wang F, Deng Y, Zhou Y, Dong J, Chen H, Dong Y, Wang N, Li X, Li H - PLoS ONE (2015)

Domain analysis of soybean PLCs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589352&req=5

pone.0138467.g001: Domain analysis of soybean PLCs.
Mentions: To characterize the soybean PLC genes, their domain structure was analyzed and a phylogenetic tree was constructed. PLC proteins contain a Pleckstrin Homology (PH) domain, EF hand, catalytic domain and C2 domain in animals. Some also contain a PDZ binding motif, Ras-binding domain, guanine-nucleotide-exchange factor for the Ras domain and Src homology domain [5]. However, in plants, such as soybean, PLCs only contain an EF hand, catalytic domain and C2 domain (Fig 1). The EF hand, which also found in DGK, has a key role in Ca2+ binding [34]. The catalytic domain, which contains X and Y domains, is conserved among eukaryotic PLCs [35], especially soybean PLC (S2 Fig). The C2 domain can bind Ca2+ and other effectors, including phospholipids, inositol phosphates, and proteins [36–38]. The C2 domain appears at the beginning of phospholipase D [39], but at the end of soybean PLC (Fig 1).

Bottom Line: Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins.The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically.It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

ABSTRACT
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8-70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.

No MeSH data available.