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Extensive Hair-Shaft Elongation by Isolated Mouse Whisker Follicles in Very Long-Term Gelfoam® Histoculture.

Cao W, Li L, Mii S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Bottom Line: By day-63 in histoculture, the number of ND-GFP hair follicle stem cells increased significantly and the follicles were intact.The present study shows that Gelfoam® histoculture can support extensive hair-shaft growth as well as hair follicle sensory-nerve growth from isolated hair follicles which were maintained over very long periods of time.Gelfoam® histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, United States of America; Department of Surgery, University of California San Diego, San Diego, CA, United States of America; Department of Anatomy, Second Military Medical University, Shanghai, China.

ABSTRACT
We have previously studied mouse whisker follicles in Gelfoam® histoculture to determine the role of nestin-expressing plutipotent stem cells, located within the follicle, in the growth of the follicular sensory nerve. Long-term Gelfoam® whisker histoculture enabled hair follicle nestin-expressing stem cells to promote the extensive elongation of the whisker sensory nerve, which contained axon fibers. Transgenic mice in which the nestin promoter drives green fluorescent protein (ND-GFP) were used as the source of the whiskers allowing imaging of the nestin-expressing stem cells as they formed the follicular sensory nerve. In the present report, we show that Gelfoam®-histocultured whisker follicles produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day-4 and continued growing until at least day-12 after which the hair-shaft length was constant. By day-63 in histoculture, the number of ND-GFP hair follicle stem cells increased significantly and the follicles were intact. The present study shows that Gelfoam® histoculture can support extensive hair-shaft growth as well as hair follicle sensory-nerve growth from isolated hair follicles which were maintained over very long periods of time. Gelfoam® histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.

No MeSH data available.


Graphs quantifying the increase of shaft length over time in individual follicles during Gelfoam® histoculture.
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pone.0138005.g002: Graphs quantifying the increase of shaft length over time in individual follicles during Gelfoam® histoculture.

Mentions: When whisker hair follicles were freshly isolated, the follicle was covered by a rigid and intact capsule filled with red blood (Fig 1). The isolated follicles with the capsule were then placed on Gelfoam® histoculture. Hair-shaft length in the follicles increased by 1.32 ± 0.27 mm by day 4 compared to day 1 and kept growing at day 7 (1.42 ± 0.24 mm) and day 9 (1.46 ± 0.24 mm) compared to day 1. By day-12, the hair shaft length was 1.50 ± 0.22 mm (p<0.001 compared to day-1) and remained constant until day 63 (Figs 1 and 2). At day-63 of hair follicle histoculture, the ND-GFP-expressing stem cell follicles had a large increase in relative fluorescence intensity and fluorescent area (p < 0.001 for both). The large increase in ND-GFP expression of the stem cells indicates their extensive proliferation and activity, as well as the very long-term viability of the follicles in Gelfoam® histoculture. Thus ND-GFP-expressing stem cells increased over the 63-day histoculture period even though hair shaft elongation appeared to cease by day 20 (Figs 2 and 3).


Extensive Hair-Shaft Elongation by Isolated Mouse Whisker Follicles in Very Long-Term Gelfoam® Histoculture.

Cao W, Li L, Mii S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Graphs quantifying the increase of shaft length over time in individual follicles during Gelfoam® histoculture.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589329&req=5

pone.0138005.g002: Graphs quantifying the increase of shaft length over time in individual follicles during Gelfoam® histoculture.
Mentions: When whisker hair follicles were freshly isolated, the follicle was covered by a rigid and intact capsule filled with red blood (Fig 1). The isolated follicles with the capsule were then placed on Gelfoam® histoculture. Hair-shaft length in the follicles increased by 1.32 ± 0.27 mm by day 4 compared to day 1 and kept growing at day 7 (1.42 ± 0.24 mm) and day 9 (1.46 ± 0.24 mm) compared to day 1. By day-12, the hair shaft length was 1.50 ± 0.22 mm (p<0.001 compared to day-1) and remained constant until day 63 (Figs 1 and 2). At day-63 of hair follicle histoculture, the ND-GFP-expressing stem cell follicles had a large increase in relative fluorescence intensity and fluorescent area (p < 0.001 for both). The large increase in ND-GFP expression of the stem cells indicates their extensive proliferation and activity, as well as the very long-term viability of the follicles in Gelfoam® histoculture. Thus ND-GFP-expressing stem cells increased over the 63-day histoculture period even though hair shaft elongation appeared to cease by day 20 (Figs 2 and 3).

Bottom Line: By day-63 in histoculture, the number of ND-GFP hair follicle stem cells increased significantly and the follicles were intact.The present study shows that Gelfoam® histoculture can support extensive hair-shaft growth as well as hair follicle sensory-nerve growth from isolated hair follicles which were maintained over very long periods of time.Gelfoam® histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, United States of America; Department of Surgery, University of California San Diego, San Diego, CA, United States of America; Department of Anatomy, Second Military Medical University, Shanghai, China.

ABSTRACT
We have previously studied mouse whisker follicles in Gelfoam® histoculture to determine the role of nestin-expressing plutipotent stem cells, located within the follicle, in the growth of the follicular sensory nerve. Long-term Gelfoam® whisker histoculture enabled hair follicle nestin-expressing stem cells to promote the extensive elongation of the whisker sensory nerve, which contained axon fibers. Transgenic mice in which the nestin promoter drives green fluorescent protein (ND-GFP) were used as the source of the whiskers allowing imaging of the nestin-expressing stem cells as they formed the follicular sensory nerve. In the present report, we show that Gelfoam®-histocultured whisker follicles produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day-4 and continued growing until at least day-12 after which the hair-shaft length was constant. By day-63 in histoculture, the number of ND-GFP hair follicle stem cells increased significantly and the follicles were intact. The present study shows that Gelfoam® histoculture can support extensive hair-shaft growth as well as hair follicle sensory-nerve growth from isolated hair follicles which were maintained over very long periods of time. Gelfoam® histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.

No MeSH data available.