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Effects of Dexamethasone and Insulin Alone or in Combination on Energy and Protein Metabolism Indicators and Milk Production in Dairy Cows in Early Lactation - A Randomized Controlled Trial.

Sami M, Mohri M, Seifi HA - PLoS ONE (2015)

Bottom Line: The sera were analyzed for β-hydroxybutyrate (BHBA), nonesterified fatty acids (NEFA), glucose, cholesterol, albumin, urea, and aspartate amino transferase (AST).There was no significant interaction of group of treatment and time of intervention (day 3 or 10 post-partum) on serum components.There were no treatment effects on test-day milk production, milk fat and protein percentages.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-1793, Mashhad, Iran.

ABSTRACT

Objectives: This study investigated the effects of dexamethasone and insulin, when administered at 3rd or 10th day of lactation on energy and protein metabolism in dairy cows.

Materials and methods: Two hundred Holstein cows were enrolled in a randomized controlled clinical trial. The cows were randomly assigned to receive 1 of 4 treatments at 3 or 10 days in milk: control group, 10-mL i.m. injection of sterile water, group insulin, s.c. injection of 100 units of insulin, group dexamethasone, i.m. injection of 20 mg of dexamethasone, group insulin plus dexamethasone, i.m. injection of 20 mg of dexamethasone and 100 units of insulin. The cows randomly assigned to receive the treatments on 3 or 10 days of lactation. Serum samples obtained at the time of enrollment, time of treatment and at 2, 4, 7 and 14 days after intervention. The sera were analyzed for β-hydroxybutyrate (BHBA), nonesterified fatty acids (NEFA), glucose, cholesterol, albumin, urea, and aspartate amino transferase (AST). Data were analyzed using a repeated measures mixed model that accounted for the effects of parity, body condition score, dystocia, retained placenta, metritis and the random effect of cow.

Results: There was no significant interaction of group of treatment and time of intervention (day 3 or 10 post-partum) on serum components. Cows that received insulin or dexamethasone alone or in combination, had lower BHBA 2 days after treatment compared with control cows, whereas concentrations of NEFA, were unaffected suggesting that glucocorticoids lipolytic effects do not appear to be important in healthy cows. AST activities significantly reduced in cows that received dexamethasone with or without insulin at 2 and 4 days after treatment. Albumin and urea concentrations 2 days after treatment were higher for cows that received dexamethasone only or dexamethasone plus insulin compared with control and Ins received cows. There were no treatment effects on test-day milk production, milk fat and protein percentages.

Conclusions: The results suggested that administration of glucocorticoids in early lactation resulted in short-term improvement of metabolism in postpartum dairy cows in biochemical terms.

No MeSH data available.


Related in: MedlinePlus

Least square mean and standard error of serum concentrations of (a) BHBA, (b) NEFA, (c) glucose, (d) cholesterol, (e) aspartate aminotransferase (AST), (f) urea and (g) albumin in 193 cows received placebo (blue diamond, 49 cows), insulin (red square, 47 cows), dexamethasone (green triangle, 49 cows), and insulin plus dexamethasone (purple circle, 48 cows).Treatments were given once at +3 or +10 d relating to calving. Significant differences (P<0·0083) are marked *.
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pone.0139276.g001: Least square mean and standard error of serum concentrations of (a) BHBA, (b) NEFA, (c) glucose, (d) cholesterol, (e) aspartate aminotransferase (AST), (f) urea and (g) albumin in 193 cows received placebo (blue diamond, 49 cows), insulin (red square, 47 cows), dexamethasone (green triangle, 49 cows), and insulin plus dexamethasone (purple circle, 48 cows).Treatments were given once at +3 or +10 d relating to calving. Significant differences (P<0·0083) are marked *.

Mentions: Results for serum BHBA, NEFA, glucose, albumin, urea, and cholesterol concentrations and AST activities are presented in Fig 1. Time effect was significant for all metabolite variables. Interaction of time of intervention × treatment was not significant for all metabolites. Treatment × time interactions were significant for serum BHBA, glucose, AST, urea, albumin and cholesterol concentrations. All comparisons of treatment groups with controls were accounted to the effects of time of intervention, parity group, BCS category, dystocia, retained placenta, and metritis. Effects of various interventions on serum BHBA, NEFA, AST, glucose, cholesterol, urea and albumin concentrations are presented in Fig 1.


Effects of Dexamethasone and Insulin Alone or in Combination on Energy and Protein Metabolism Indicators and Milk Production in Dairy Cows in Early Lactation - A Randomized Controlled Trial.

Sami M, Mohri M, Seifi HA - PLoS ONE (2015)

Least square mean and standard error of serum concentrations of (a) BHBA, (b) NEFA, (c) glucose, (d) cholesterol, (e) aspartate aminotransferase (AST), (f) urea and (g) albumin in 193 cows received placebo (blue diamond, 49 cows), insulin (red square, 47 cows), dexamethasone (green triangle, 49 cows), and insulin plus dexamethasone (purple circle, 48 cows).Treatments were given once at +3 or +10 d relating to calving. Significant differences (P<0·0083) are marked *.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589323&req=5

pone.0139276.g001: Least square mean and standard error of serum concentrations of (a) BHBA, (b) NEFA, (c) glucose, (d) cholesterol, (e) aspartate aminotransferase (AST), (f) urea and (g) albumin in 193 cows received placebo (blue diamond, 49 cows), insulin (red square, 47 cows), dexamethasone (green triangle, 49 cows), and insulin plus dexamethasone (purple circle, 48 cows).Treatments were given once at +3 or +10 d relating to calving. Significant differences (P<0·0083) are marked *.
Mentions: Results for serum BHBA, NEFA, glucose, albumin, urea, and cholesterol concentrations and AST activities are presented in Fig 1. Time effect was significant for all metabolite variables. Interaction of time of intervention × treatment was not significant for all metabolites. Treatment × time interactions were significant for serum BHBA, glucose, AST, urea, albumin and cholesterol concentrations. All comparisons of treatment groups with controls were accounted to the effects of time of intervention, parity group, BCS category, dystocia, retained placenta, and metritis. Effects of various interventions on serum BHBA, NEFA, AST, glucose, cholesterol, urea and albumin concentrations are presented in Fig 1.

Bottom Line: The sera were analyzed for β-hydroxybutyrate (BHBA), nonesterified fatty acids (NEFA), glucose, cholesterol, albumin, urea, and aspartate amino transferase (AST).There was no significant interaction of group of treatment and time of intervention (day 3 or 10 post-partum) on serum components.There were no treatment effects on test-day milk production, milk fat and protein percentages.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-1793, Mashhad, Iran.

ABSTRACT

Objectives: This study investigated the effects of dexamethasone and insulin, when administered at 3rd or 10th day of lactation on energy and protein metabolism in dairy cows.

Materials and methods: Two hundred Holstein cows were enrolled in a randomized controlled clinical trial. The cows were randomly assigned to receive 1 of 4 treatments at 3 or 10 days in milk: control group, 10-mL i.m. injection of sterile water, group insulin, s.c. injection of 100 units of insulin, group dexamethasone, i.m. injection of 20 mg of dexamethasone, group insulin plus dexamethasone, i.m. injection of 20 mg of dexamethasone and 100 units of insulin. The cows randomly assigned to receive the treatments on 3 or 10 days of lactation. Serum samples obtained at the time of enrollment, time of treatment and at 2, 4, 7 and 14 days after intervention. The sera were analyzed for β-hydroxybutyrate (BHBA), nonesterified fatty acids (NEFA), glucose, cholesterol, albumin, urea, and aspartate amino transferase (AST). Data were analyzed using a repeated measures mixed model that accounted for the effects of parity, body condition score, dystocia, retained placenta, metritis and the random effect of cow.

Results: There was no significant interaction of group of treatment and time of intervention (day 3 or 10 post-partum) on serum components. Cows that received insulin or dexamethasone alone or in combination, had lower BHBA 2 days after treatment compared with control cows, whereas concentrations of NEFA, were unaffected suggesting that glucocorticoids lipolytic effects do not appear to be important in healthy cows. AST activities significantly reduced in cows that received dexamethasone with or without insulin at 2 and 4 days after treatment. Albumin and urea concentrations 2 days after treatment were higher for cows that received dexamethasone only or dexamethasone plus insulin compared with control and Ins received cows. There were no treatment effects on test-day milk production, milk fat and protein percentages.

Conclusions: The results suggested that administration of glucocorticoids in early lactation resulted in short-term improvement of metabolism in postpartum dairy cows in biochemical terms.

No MeSH data available.


Related in: MedlinePlus