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Analysis of Transmission of MRSA and ESBL-E among Pigs and Farm Personnel.

Schmithausen RM, Schulze-Geisthoevel SV, Stemmer F, El-Jade M, Reif M, Hack S, Meilaender A, Montabauer G, Fimmers R, Parcina M, Hoerauf A, Exner M, Petersen B, Bierbaum G, Bekeredjian-Ding I - PLoS ONE (2015)

Bottom Line: ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found.Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms.Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany; Institute of Animal Science, Preventive Health Management Group, University of Bonn, Katzenburgweg 7-9, Bonn, Germany.

ABSTRACT
Livestock-associated bacteria with resistance to two or more antibiotic drug classes have heightened our awareness for the consequences of antibiotic consumption and spread of resistant bacterial strains in the veterinary field. In this study we assessed the prevalence of concomitant colonization with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) and enterobacteriaceae expressing extended-spectrum betalactamases (ESBL-E) in farms at the German-Dutch border region. Nasal colonization of pigs with MRSA (113/547 (20.7%)) was less frequent than rectal colonization with ESBL-E (163/540 (30.2%)). On the individual farm level MRSA correlated with ESBL-E recovery. The data further provide information on prevalence at different stages of pig production, including abattoirs, as well as in air samples and humans living and working on the farms. Notably, MRSA was detected in stable air samples of 34 out of 35 pig farms, highlighting air as an important MRSA transmission reservoir. The majority of MRSA isolates, including those from humans, displayed tetracycline resistance and spa types t011 and t034 characteristic for LA-MRSA, demonstrating transmission from pigs to humans. ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found. Detection of ESBL-E, e.g. mostly Escherichia coli with CTX-M-type ESBL, was limited to these six farms. Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms. Although our data suggest that acquisition of MRSA and ESBL-E might occur among pigs in the abattoirs, MRSA and ESBL-E were not detected on the carcasses. Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

No MeSH data available.


Related in: MedlinePlus

spa typing of MRSA isolates.A:spa types of MRSA isolates obtained from pigs, human and air. B: Prevalence of spa types detected on farms and on abattoirs. C: Analysis of spa types in regard to their simultaneous presence in different media (pig, human, air).
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pone.0138173.g003: spa typing of MRSA isolates.A:spa types of MRSA isolates obtained from pigs, human and air. B: Prevalence of spa types detected on farms and on abattoirs. C: Analysis of spa types in regard to their simultaneous presence in different media (pig, human, air).

Mentions: To confirm the LA-MRSA lineage of the isolates we performed spa typing (Fig 3A). The spa types most frequently isolated from snouts and air were t011 (n = 130) and t034 (n = 35) (Fig 3B; S9 Table). All MRSA spa types belonged to the ST398 lineage. Only two MSSA isolates recovered from human nasal swabs corresponded to spa types t005 and t491 that do not belong to this lineage.


Analysis of Transmission of MRSA and ESBL-E among Pigs and Farm Personnel.

Schmithausen RM, Schulze-Geisthoevel SV, Stemmer F, El-Jade M, Reif M, Hack S, Meilaender A, Montabauer G, Fimmers R, Parcina M, Hoerauf A, Exner M, Petersen B, Bierbaum G, Bekeredjian-Ding I - PLoS ONE (2015)

spa typing of MRSA isolates.A:spa types of MRSA isolates obtained from pigs, human and air. B: Prevalence of spa types detected on farms and on abattoirs. C: Analysis of spa types in regard to their simultaneous presence in different media (pig, human, air).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589321&req=5

pone.0138173.g003: spa typing of MRSA isolates.A:spa types of MRSA isolates obtained from pigs, human and air. B: Prevalence of spa types detected on farms and on abattoirs. C: Analysis of spa types in regard to their simultaneous presence in different media (pig, human, air).
Mentions: To confirm the LA-MRSA lineage of the isolates we performed spa typing (Fig 3A). The spa types most frequently isolated from snouts and air were t011 (n = 130) and t034 (n = 35) (Fig 3B; S9 Table). All MRSA spa types belonged to the ST398 lineage. Only two MSSA isolates recovered from human nasal swabs corresponded to spa types t005 and t491 that do not belong to this lineage.

Bottom Line: ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found.Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms.Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany; Institute of Animal Science, Preventive Health Management Group, University of Bonn, Katzenburgweg 7-9, Bonn, Germany.

ABSTRACT
Livestock-associated bacteria with resistance to two or more antibiotic drug classes have heightened our awareness for the consequences of antibiotic consumption and spread of resistant bacterial strains in the veterinary field. In this study we assessed the prevalence of concomitant colonization with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) and enterobacteriaceae expressing extended-spectrum betalactamases (ESBL-E) in farms at the German-Dutch border region. Nasal colonization of pigs with MRSA (113/547 (20.7%)) was less frequent than rectal colonization with ESBL-E (163/540 (30.2%)). On the individual farm level MRSA correlated with ESBL-E recovery. The data further provide information on prevalence at different stages of pig production, including abattoirs, as well as in air samples and humans living and working on the farms. Notably, MRSA was detected in stable air samples of 34 out of 35 pig farms, highlighting air as an important MRSA transmission reservoir. The majority of MRSA isolates, including those from humans, displayed tetracycline resistance and spa types t011 and t034 characteristic for LA-MRSA, demonstrating transmission from pigs to humans. ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found. Detection of ESBL-E, e.g. mostly Escherichia coli with CTX-M-type ESBL, was limited to these six farms. Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms. Although our data suggest that acquisition of MRSA and ESBL-E might occur among pigs in the abattoirs, MRSA and ESBL-E were not detected on the carcasses. Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

No MeSH data available.


Related in: MedlinePlus