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Analysis of Transmission of MRSA and ESBL-E among Pigs and Farm Personnel.

Schmithausen RM, Schulze-Geisthoevel SV, Stemmer F, El-Jade M, Reif M, Hack S, Meilaender A, Montabauer G, Fimmers R, Parcina M, Hoerauf A, Exner M, Petersen B, Bierbaum G, Bekeredjian-Ding I - PLoS ONE (2015)

Bottom Line: ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found.Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms.Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany; Institute of Animal Science, Preventive Health Management Group, University of Bonn, Katzenburgweg 7-9, Bonn, Germany.

ABSTRACT
Livestock-associated bacteria with resistance to two or more antibiotic drug classes have heightened our awareness for the consequences of antibiotic consumption and spread of resistant bacterial strains in the veterinary field. In this study we assessed the prevalence of concomitant colonization with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) and enterobacteriaceae expressing extended-spectrum betalactamases (ESBL-E) in farms at the German-Dutch border region. Nasal colonization of pigs with MRSA (113/547 (20.7%)) was less frequent than rectal colonization with ESBL-E (163/540 (30.2%)). On the individual farm level MRSA correlated with ESBL-E recovery. The data further provide information on prevalence at different stages of pig production, including abattoirs, as well as in air samples and humans living and working on the farms. Notably, MRSA was detected in stable air samples of 34 out of 35 pig farms, highlighting air as an important MRSA transmission reservoir. The majority of MRSA isolates, including those from humans, displayed tetracycline resistance and spa types t011 and t034 characteristic for LA-MRSA, demonstrating transmission from pigs to humans. ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found. Detection of ESBL-E, e.g. mostly Escherichia coli with CTX-M-type ESBL, was limited to these six farms. Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms. Although our data suggest that acquisition of MRSA and ESBL-E might occur among pigs in the abattoirs, MRSA and ESBL-E were not detected on the carcasses. Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

No MeSH data available.


Related in: MedlinePlus

Comparative overview of MRSA and ESBL-E colonization in pigs, humans and air on farms.Samples from pig, human and air were collected on 35 pig farms (B#). Farm types (farrowing (FR), nursery (NF) and finishing (FF)) are provided in the diagram. All samples were analyzed for MRSA (A) and ESBL-E (B). The figure depicts the results obtained on the individual farms sorted by prevalence of MRSA (A) or ESBL-E (B), respectively. Bars depict the percentage of positive (black) and negative (gray) samples.
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pone.0138173.g001: Comparative overview of MRSA and ESBL-E colonization in pigs, humans and air on farms.Samples from pig, human and air were collected on 35 pig farms (B#). Farm types (farrowing (FR), nursery (NF) and finishing (FF)) are provided in the diagram. All samples were analyzed for MRSA (A) and ESBL-E (B). The figure depicts the results obtained on the individual farms sorted by prevalence of MRSA (A) or ESBL-E (B), respectively. Bars depict the percentage of positive (black) and negative (gray) samples.

Mentions: We analyzed the frequency of MRSA and ESBL-E in samples obtained from pigs from 35 farms located in the German-Dutch border region as well as two associated abattoirs, the respective farm environment (e.g. air) and persons living and working on these farms (see Fig 1 for overview).


Analysis of Transmission of MRSA and ESBL-E among Pigs and Farm Personnel.

Schmithausen RM, Schulze-Geisthoevel SV, Stemmer F, El-Jade M, Reif M, Hack S, Meilaender A, Montabauer G, Fimmers R, Parcina M, Hoerauf A, Exner M, Petersen B, Bierbaum G, Bekeredjian-Ding I - PLoS ONE (2015)

Comparative overview of MRSA and ESBL-E colonization in pigs, humans and air on farms.Samples from pig, human and air were collected on 35 pig farms (B#). Farm types (farrowing (FR), nursery (NF) and finishing (FF)) are provided in the diagram. All samples were analyzed for MRSA (A) and ESBL-E (B). The figure depicts the results obtained on the individual farms sorted by prevalence of MRSA (A) or ESBL-E (B), respectively. Bars depict the percentage of positive (black) and negative (gray) samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4589321&req=5

pone.0138173.g001: Comparative overview of MRSA and ESBL-E colonization in pigs, humans and air on farms.Samples from pig, human and air were collected on 35 pig farms (B#). Farm types (farrowing (FR), nursery (NF) and finishing (FF)) are provided in the diagram. All samples were analyzed for MRSA (A) and ESBL-E (B). The figure depicts the results obtained on the individual farms sorted by prevalence of MRSA (A) or ESBL-E (B), respectively. Bars depict the percentage of positive (black) and negative (gray) samples.
Mentions: We analyzed the frequency of MRSA and ESBL-E in samples obtained from pigs from 35 farms located in the German-Dutch border region as well as two associated abattoirs, the respective farm environment (e.g. air) and persons living and working on these farms (see Fig 1 for overview).

Bottom Line: ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found.Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms.Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany; Institute of Animal Science, Preventive Health Management Group, University of Bonn, Katzenburgweg 7-9, Bonn, Germany.

ABSTRACT
Livestock-associated bacteria with resistance to two or more antibiotic drug classes have heightened our awareness for the consequences of antibiotic consumption and spread of resistant bacterial strains in the veterinary field. In this study we assessed the prevalence of concomitant colonization with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) and enterobacteriaceae expressing extended-spectrum betalactamases (ESBL-E) in farms at the German-Dutch border region. Nasal colonization of pigs with MRSA (113/547 (20.7%)) was less frequent than rectal colonization with ESBL-E (163/540 (30.2%)). On the individual farm level MRSA correlated with ESBL-E recovery. The data further provide information on prevalence at different stages of pig production, including abattoirs, as well as in air samples and humans living and working on the farms. Notably, MRSA was detected in stable air samples of 34 out of 35 pig farms, highlighting air as an important MRSA transmission reservoir. The majority of MRSA isolates, including those from humans, displayed tetracycline resistance and spa types t011 and t034 characteristic for LA-MRSA, demonstrating transmission from pigs to humans. ESBL-E positive air samples were detected on 6 out of 35 farms but no pig-to-human transmission was found. Detection of ESBL-E, e.g. mostly Escherichia coli with CTX-M-type ESBL, was limited to these six farms. Molecular typing revealed transmission of ESBL-E within the pig compartments; however, related strains were also found on unrelated farms. Although our data suggest that acquisition of MRSA and ESBL-E might occur among pigs in the abattoirs, MRSA and ESBL-E were not detected on the carcasses. Altogether, our data define stable air (MRSA), pig compartments (ESBL-E) and abattoir waiting areas (MRSA and ESBL-E) as major hot spots for transmission of MRSA and/or ESBL-E along the pig production chain.

No MeSH data available.


Related in: MedlinePlus