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Immune profiling in human breast cancer using high-sensitivity detection and analysis techniques.

Coventry BJ, Weightman MJ, Bradley J, Skinner JM - JRSM Open (2015)

Bottom Line: CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01).CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences.Further correlation of these findings with clinical outcome, including survival, is proceeding with encouraging results.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Surgery, University of Adelaide, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia.

ABSTRACT

Objectives: Evaluation of immune profiles in human breast cancer using high-sensitivity detection and analysis methods.

Design: Cohort comparative analysis studies of breast tissue.

Setting: Human hospital and laboratory healthcare facilities.

Participants: Women over 18 years.

Main outcome measures: Evaluation of the comparative immunophenotype of human breast carcinoma and normal breast tissues.

Results: Leukocyte density and specific subgroups of lymphocytes and macrophages were generally higher in breast cancers compared to normal breast tissues. CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01). Some 30% of T-cells were γδ-TCR positive, but the majority were αβ-TCR in type. CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences. IL-2α receptor expression was low or absent on most TIL.

Conclusions: High-sensitivity and image analysis techniques permitted accurate characterisation of the TIL infiltrate for immune profiling. Breast carcinoma showed predominance of CD4 T-cells of mainly memory phenotype. Normal breast tissues showed low leukocyte infiltration. Further correlation of these findings with clinical outcome, including survival, is proceeding with encouraging results.

No MeSH data available.


Related in: MedlinePlus

Histograms comparing manual visual grades of infiltrate found in normal breast and primary breast carcinoma tissues with respect to immunostaining for various antigens.
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fig1-2054270415603909: Histograms comparing manual visual grades of infiltrate found in normal breast and primary breast carcinoma tissues with respect to immunostaining for various antigens.

Mentions: The frequency percentage of visual grades assigned for the 25 breast carcinoma and 10 normal breast tissue samples tissues were represented on histograms (Figure 1) displaying each grade of cellular infiltrate for each specific monoclonal antibody.


Immune profiling in human breast cancer using high-sensitivity detection and analysis techniques.

Coventry BJ, Weightman MJ, Bradley J, Skinner JM - JRSM Open (2015)

Histograms comparing manual visual grades of infiltrate found in normal breast and primary breast carcinoma tissues with respect to immunostaining for various antigens.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4589078&req=5

fig1-2054270415603909: Histograms comparing manual visual grades of infiltrate found in normal breast and primary breast carcinoma tissues with respect to immunostaining for various antigens.
Mentions: The frequency percentage of visual grades assigned for the 25 breast carcinoma and 10 normal breast tissue samples tissues were represented on histograms (Figure 1) displaying each grade of cellular infiltrate for each specific monoclonal antibody.

Bottom Line: CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01).CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences.Further correlation of these findings with clinical outcome, including survival, is proceeding with encouraging results.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Surgery, University of Adelaide, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia.

ABSTRACT

Objectives: Evaluation of immune profiles in human breast cancer using high-sensitivity detection and analysis methods.

Design: Cohort comparative analysis studies of breast tissue.

Setting: Human hospital and laboratory healthcare facilities.

Participants: Women over 18 years.

Main outcome measures: Evaluation of the comparative immunophenotype of human breast carcinoma and normal breast tissues.

Results: Leukocyte density and specific subgroups of lymphocytes and macrophages were generally higher in breast cancers compared to normal breast tissues. CD3, CD4, CD45RO, CD45RA(2H4), CD45 and HLA Class II (on TIL) were significantly expressed on breast tumour tissues compared with normal tissues (p < .01). Some 30% of T-cells were γδ-TCR positive, but the majority were αβ-TCR in type. CD19 (B-cell), CD14 (FMC32 and 33) and HLA Class I levels (epithelial and TIL) showed no significant differences. IL-2α receptor expression was low or absent on most TIL.

Conclusions: High-sensitivity and image analysis techniques permitted accurate characterisation of the TIL infiltrate for immune profiling. Breast carcinoma showed predominance of CD4 T-cells of mainly memory phenotype. Normal breast tissues showed low leukocyte infiltration. Further correlation of these findings with clinical outcome, including survival, is proceeding with encouraging results.

No MeSH data available.


Related in: MedlinePlus