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Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia.

Laohasinnarong D, Goto Y, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B - Parasit Vectors (2015)

Bottom Line: Infection of both cattle and goats with Trypanosoma congolense and T. vivax was associated with clinical AAT.When selecting molecular techniques for AAT surveillance in endemic regions, the KIN-PCR and species-specific PCR may be recommended for screening animal or tsetse fly samples for T. congolense and T. vivax, respectively.On the other hand, species-specific PCR and/or LAMP might be of greater value in the screening of animal and human body fluids as well as tsetse fly samples for Trypanozoon.

View Article: PubMed Central - PubMed

Affiliation: O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan. dusit.lao@mahidol.ac.th.

ABSTRACT

Background: The present study, conducted in Zambia's Luangwa valley where both animal African trypanosomiasis (AAT) and human African trypanosomiasis (HAT) are endemic, combined the use of microscopy and molecular techniques to determine the presence of trypanosome species in cattle, goats and tsetse flies.

Methods: This study was conducted between 2008 and 2010 in Petauke, Chama and Isoka districts, north-eastern Zambia. A total of 243 cattle, 36 goats and 546 tsetse flies, were examined for presence of trypanosome species using microscopy, PCR and loop-mediated isothermal amplification (LAMP).

Results: There was poor agreement among the test methods used for detection of trypanosomes species in animal blood and tsetse flies. Trypanosomes were observed in 6.1 % (95 % CI: 3.3-8.9 %) of the animals sampled by microscopy, 7.5 % (95 % CI: 4.4-10.6 %) by PCR and 18.6 % (95 % CI: 13.6-23.6 %) by PFR-LAMP. PFR-LAMP was more sensitive for detecting Trypanozoon than KIN-PCR. The highest occurrence of AAT was recorded in cattle from Petauke (58.7 %, 95 % CI: 44.7-72.7 %) while the lowest was from Isoka (5.4 %, 95 % CI: 0.8-10.0 %). Infection of both cattle and goats with Trypanosoma congolense and T. vivax was associated with clinical AAT.

Conclusion: When selecting molecular techniques for AAT surveillance in endemic regions, the KIN-PCR and species-specific PCR may be recommended for screening animal or tsetse fly samples for T. congolense and T. vivax, respectively. On the other hand, species-specific PCR and/or LAMP might be of greater value in the screening of animal and human body fluids as well as tsetse fly samples for Trypanozoon.

No MeSH data available.


Related in: MedlinePlus

Map of Zambia showing location of Isoka, Chama and Petauke districts
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Fig1: Map of Zambia showing location of Isoka, Chama and Petauke districts

Mentions: Studies were conducted between May and October in 2008 and 2010 in Petauke (Eastern Province), Chama (Muchinga Province) and Isoka (Northern Province) districts, north-eastern Zambia (Fig. 1). A total of 243 indigenous and mixed breeds of cattle from Isoka (92), Petauke (46) and Chama (105) and 36 indigenous breeds of goats (all from Chama), were examined for presence of trypanosome species using microscopy, PCR and LAMP assays. In addition, PCR and LAMP assays were used to examine the presence of trypanosome species in 546 tsetse samples (caught from Mbambanda Zaro sanctuary, Chama) comprising 492 Glossina m. morsitans (27 males and 465 females) and 54 G. pallidipes (all males). Mbambanda Zaro sanctuary, lying on the border with Malawi, is part of the Zambia wildlife authority (ZAWA). It has abundant wildlife and tsetse flies and is surrounded by human settlements that maintain limited livestock, mainly goats.Fig. 1


Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia.

Laohasinnarong D, Goto Y, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B - Parasit Vectors (2015)

Map of Zambia showing location of Isoka, Chama and Petauke districts
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4589067&req=5

Fig1: Map of Zambia showing location of Isoka, Chama and Petauke districts
Mentions: Studies were conducted between May and October in 2008 and 2010 in Petauke (Eastern Province), Chama (Muchinga Province) and Isoka (Northern Province) districts, north-eastern Zambia (Fig. 1). A total of 243 indigenous and mixed breeds of cattle from Isoka (92), Petauke (46) and Chama (105) and 36 indigenous breeds of goats (all from Chama), were examined for presence of trypanosome species using microscopy, PCR and LAMP assays. In addition, PCR and LAMP assays were used to examine the presence of trypanosome species in 546 tsetse samples (caught from Mbambanda Zaro sanctuary, Chama) comprising 492 Glossina m. morsitans (27 males and 465 females) and 54 G. pallidipes (all males). Mbambanda Zaro sanctuary, lying on the border with Malawi, is part of the Zambia wildlife authority (ZAWA). It has abundant wildlife and tsetse flies and is surrounded by human settlements that maintain limited livestock, mainly goats.Fig. 1

Bottom Line: Infection of both cattle and goats with Trypanosoma congolense and T. vivax was associated with clinical AAT.When selecting molecular techniques for AAT surveillance in endemic regions, the KIN-PCR and species-specific PCR may be recommended for screening animal or tsetse fly samples for T. congolense and T. vivax, respectively.On the other hand, species-specific PCR and/or LAMP might be of greater value in the screening of animal and human body fluids as well as tsetse fly samples for Trypanozoon.

View Article: PubMed Central - PubMed

Affiliation: O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan. dusit.lao@mahidol.ac.th.

ABSTRACT

Background: The present study, conducted in Zambia's Luangwa valley where both animal African trypanosomiasis (AAT) and human African trypanosomiasis (HAT) are endemic, combined the use of microscopy and molecular techniques to determine the presence of trypanosome species in cattle, goats and tsetse flies.

Methods: This study was conducted between 2008 and 2010 in Petauke, Chama and Isoka districts, north-eastern Zambia. A total of 243 cattle, 36 goats and 546 tsetse flies, were examined for presence of trypanosome species using microscopy, PCR and loop-mediated isothermal amplification (LAMP).

Results: There was poor agreement among the test methods used for detection of trypanosomes species in animal blood and tsetse flies. Trypanosomes were observed in 6.1 % (95 % CI: 3.3-8.9 %) of the animals sampled by microscopy, 7.5 % (95 % CI: 4.4-10.6 %) by PCR and 18.6 % (95 % CI: 13.6-23.6 %) by PFR-LAMP. PFR-LAMP was more sensitive for detecting Trypanozoon than KIN-PCR. The highest occurrence of AAT was recorded in cattle from Petauke (58.7 %, 95 % CI: 44.7-72.7 %) while the lowest was from Isoka (5.4 %, 95 % CI: 0.8-10.0 %). Infection of both cattle and goats with Trypanosoma congolense and T. vivax was associated with clinical AAT.

Conclusion: When selecting molecular techniques for AAT surveillance in endemic regions, the KIN-PCR and species-specific PCR may be recommended for screening animal or tsetse fly samples for T. congolense and T. vivax, respectively. On the other hand, species-specific PCR and/or LAMP might be of greater value in the screening of animal and human body fluids as well as tsetse fly samples for Trypanozoon.

No MeSH data available.


Related in: MedlinePlus