Limits...
miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus

Expression of Ezrin and miR-96 in metastatic and non- metastatic renal cancer patients. a miR-96 was lower in patients who developed metastasis than in those who remained in remission. miR-96 expression was detected by quantitative RT-PCR (* p < 0.05). b Ezrin was higher in patients who developed metastasis than in those who remained in remission. Ezrin was detected by western blotting and normalized to GAPDH. IntDen ratio of Ezrin to GAPDH represents the integrated density ratio as quantified with Image J software. c Pearson correlation analysis of the association between miR-96 and Ezrin in renal cancer patients showed r = −0.69 (* p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588898&req=5

Fig7: Expression of Ezrin and miR-96 in metastatic and non- metastatic renal cancer patients. a miR-96 was lower in patients who developed metastasis than in those who remained in remission. miR-96 expression was detected by quantitative RT-PCR (* p < 0.05). b Ezrin was higher in patients who developed metastasis than in those who remained in remission. Ezrin was detected by western blotting and normalized to GAPDH. IntDen ratio of Ezrin to GAPDH represents the integrated density ratio as quantified with Image J software. c Pearson correlation analysis of the association between miR-96 and Ezrin in renal cancer patients showed r = −0.69 (* p < 0.05)

Mentions: To further investigate the relationship among levels of miR-96, Ezrin and metastasis in a clinical setting, 63 human localized clear cell RCC specimens were analyzed. Using 2010 AJCC TNM staging, there were 39 stage 1 and 24 stage 2 patients. Furhman grades of the 63 RCC were 9 of grade Furhman1, 27 of Furhman 2, 19 of Furhman 3 and 8 of Furhman 4. Seventeen patients developed metastasis, whereas the remaining 46 maintained remission during a median follow-up period of 37 months. The expression levels of miR-96 and Ezrin were compared between patients who developed metastasis and those in remission. As shown in Fig. 7, metastatic patients showed lower levels of miR-96 (P < 0.05) and higher levels of Ezrin expression (P < 0.05). Pearson correlation analysis of the association between miR-96 and Ezrin in these 63 patients showed a correlation coefficient of–0.69 (P < 0.05).Fig. 7


miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Expression of Ezrin and miR-96 in metastatic and non- metastatic renal cancer patients. a miR-96 was lower in patients who developed metastasis than in those who remained in remission. miR-96 expression was detected by quantitative RT-PCR (* p < 0.05). b Ezrin was higher in patients who developed metastasis than in those who remained in remission. Ezrin was detected by western blotting and normalized to GAPDH. IntDen ratio of Ezrin to GAPDH represents the integrated density ratio as quantified with Image J software. c Pearson correlation analysis of the association between miR-96 and Ezrin in renal cancer patients showed r = −0.69 (* p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588898&req=5

Fig7: Expression of Ezrin and miR-96 in metastatic and non- metastatic renal cancer patients. a miR-96 was lower in patients who developed metastasis than in those who remained in remission. miR-96 expression was detected by quantitative RT-PCR (* p < 0.05). b Ezrin was higher in patients who developed metastasis than in those who remained in remission. Ezrin was detected by western blotting and normalized to GAPDH. IntDen ratio of Ezrin to GAPDH represents the integrated density ratio as quantified with Image J software. c Pearson correlation analysis of the association between miR-96 and Ezrin in renal cancer patients showed r = −0.69 (* p < 0.05)
Mentions: To further investigate the relationship among levels of miR-96, Ezrin and metastasis in a clinical setting, 63 human localized clear cell RCC specimens were analyzed. Using 2010 AJCC TNM staging, there were 39 stage 1 and 24 stage 2 patients. Furhman grades of the 63 RCC were 9 of grade Furhman1, 27 of Furhman 2, 19 of Furhman 3 and 8 of Furhman 4. Seventeen patients developed metastasis, whereas the remaining 46 maintained remission during a median follow-up period of 37 months. The expression levels of miR-96 and Ezrin were compared between patients who developed metastasis and those in remission. As shown in Fig. 7, metastatic patients showed lower levels of miR-96 (P < 0.05) and higher levels of Ezrin expression (P < 0.05). Pearson correlation analysis of the association between miR-96 and Ezrin in these 63 patients showed a correlation coefficient of–0.69 (P < 0.05).Fig. 7

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus