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miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus

Expression of Ezrin in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. a Ezrin was detected by western blotting using GAPDH as a loading control. b Ezrin expression increased following transfection of cells with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control. IntDen ratio represents the integrated density ratio of Ezrin to GAPDH as quantified by Image J (*p < 0.05)
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Fig5: Expression of Ezrin in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. a Ezrin was detected by western blotting using GAPDH as a loading control. b Ezrin expression increased following transfection of cells with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control. IntDen ratio represents the integrated density ratio of Ezrin to GAPDH as quantified by Image J (*p < 0.05)

Mentions: Ezrin expression was detected by western blotting in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. As shown in Fig. 5, Ezrin was significantly downregulated in Caki-1 and 786-O cells transfected with miR-96 mimic(Caki-1:0.83 ± 0.08 vs. 1.87 ± 0.18, P < 0.05; 786-O: 0.47 ± 0.04 vs.0.94 ± 0.27, P < 0.05, and was significantly upregulated in cells transfected with miR-96 inhibitor(Caki-1:2.67 ± 0.35 vs. 1.87 ± 0.18, P < 0.05; 786-O: 2.02 ± 0.39 vs.0.94 ± 0.27, P < 0.05), compared to miR-control-transfected cells.Fig. 5


miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Expression of Ezrin in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. a Ezrin was detected by western blotting using GAPDH as a loading control. b Ezrin expression increased following transfection of cells with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control. IntDen ratio represents the integrated density ratio of Ezrin to GAPDH as quantified by Image J (*p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588898&req=5

Fig5: Expression of Ezrin in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. a Ezrin was detected by western blotting using GAPDH as a loading control. b Ezrin expression increased following transfection of cells with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control. IntDen ratio represents the integrated density ratio of Ezrin to GAPDH as quantified by Image J (*p < 0.05)
Mentions: Ezrin expression was detected by western blotting in Caki-1 and 786-O cells transfected with miR-96 mimic, miR-96 inhibitor or miR-control. As shown in Fig. 5, Ezrin was significantly downregulated in Caki-1 and 786-O cells transfected with miR-96 mimic(Caki-1:0.83 ± 0.08 vs. 1.87 ± 0.18, P < 0.05; 786-O: 0.47 ± 0.04 vs.0.94 ± 0.27, P < 0.05, and was significantly upregulated in cells transfected with miR-96 inhibitor(Caki-1:2.67 ± 0.35 vs. 1.87 ± 0.18, P < 0.05; 786-O: 2.02 ± 0.39 vs.0.94 ± 0.27, P < 0.05), compared to miR-control-transfected cells.Fig. 5

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus