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miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus

Migration of Caki-1 and 786-O cells transfected with miR-96 inhibitor or miR-96 mimic compared to miR-control-transfected cells. Wound healing assay of Caki-1 cells (a) and 786-O cells (b) transfected with miR-96 inhibitor, miR-96 mimic or miR-control for 24 h. Percent healing at 24 h was calculated as the mean percentage of the remaining cell-free area compared with the area of the initial wound and showed that migration of Caki-1 (c) and 786-O (d) cells increased following transfection with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control (*p < 0.05)
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Fig3: Migration of Caki-1 and 786-O cells transfected with miR-96 inhibitor or miR-96 mimic compared to miR-control-transfected cells. Wound healing assay of Caki-1 cells (a) and 786-O cells (b) transfected with miR-96 inhibitor, miR-96 mimic or miR-control for 24 h. Percent healing at 24 h was calculated as the mean percentage of the remaining cell-free area compared with the area of the initial wound and showed that migration of Caki-1 (c) and 786-O (d) cells increased following transfection with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control (*p < 0.05)

Mentions: To evaluate the effect of changes in the levels of miR-96 on the invasive ability of RCC cells in vitro, functional assays were performed. Cell migration was assessed by wound healing assay, which showed that Caki-1 and 786-O cells transfected with the miR-96 mimic migrated at a slower rate (Caki-1: 42.2 % ± 2.7 % vs. 63.3 % ± 5.87 %, P < 0.05; 786-O: 26.9 % ± 6.35 % vs. 44.4 % ± 5.88 %, P < 0.05; Fig. 3), whereas cells transfected with miR-96 inhibitor migrated at a faster rate (Caki-1: 87.2 % ± 4.81 % vs. 63.3 % ± 5.87 %, P < 0.05; 786-O: 86.8 % ± 7.99 % vs. 44.4 % ± 5.88 %, P < 0.05; Fig. 3), compared to cells transfected with negative miR-control.Fig. 3


miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

Yu N, Fu S, Liu Y, Xu Z, Liu Y, Hao J, Wang B, Zhang A - J. Exp. Clin. Cancer Res. (2015)

Migration of Caki-1 and 786-O cells transfected with miR-96 inhibitor or miR-96 mimic compared to miR-control-transfected cells. Wound healing assay of Caki-1 cells (a) and 786-O cells (b) transfected with miR-96 inhibitor, miR-96 mimic or miR-control for 24 h. Percent healing at 24 h was calculated as the mean percentage of the remaining cell-free area compared with the area of the initial wound and showed that migration of Caki-1 (c) and 786-O (d) cells increased following transfection with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control (*p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588898&req=5

Fig3: Migration of Caki-1 and 786-O cells transfected with miR-96 inhibitor or miR-96 mimic compared to miR-control-transfected cells. Wound healing assay of Caki-1 cells (a) and 786-O cells (b) transfected with miR-96 inhibitor, miR-96 mimic or miR-control for 24 h. Percent healing at 24 h was calculated as the mean percentage of the remaining cell-free area compared with the area of the initial wound and showed that migration of Caki-1 (c) and 786-O (d) cells increased following transfection with miR-96 inhibitor, and decreased following transfection with miR-96 mimic, compared to cells transfected with miR-control (*p < 0.05)
Mentions: To evaluate the effect of changes in the levels of miR-96 on the invasive ability of RCC cells in vitro, functional assays were performed. Cell migration was assessed by wound healing assay, which showed that Caki-1 and 786-O cells transfected with the miR-96 mimic migrated at a slower rate (Caki-1: 42.2 % ± 2.7 % vs. 63.3 % ± 5.87 %, P < 0.05; 786-O: 26.9 % ± 6.35 % vs. 44.4 % ± 5.88 %, P < 0.05; Fig. 3), whereas cells transfected with miR-96 inhibitor migrated at a faster rate (Caki-1: 87.2 % ± 4.81 % vs. 63.3 % ± 5.87 %, P < 0.05; 786-O: 86.8 % ± 7.99 % vs. 44.4 % ± 5.88 %, P < 0.05; Fig. 3), compared to cells transfected with negative miR-control.Fig. 3

Bottom Line: Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. miR-96 expression was significantly lower in Caki-1compared to786-O cells.The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic.These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

View Article: PubMed Central - PubMed

Affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.

ABSTRACT

Background: The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.

Methods: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.

Results: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.

Conclusions: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.

No MeSH data available.


Related in: MedlinePlus