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Type 1 diacylglycerol acyltransferases of Brassica napus preferentially incorporate oleic acid into triacylglycerol.

Aznar-Moreno J, Denolf P, Van Audenhove K, De Bodt S, Engelen S, Fahy D, Wallis JG, Browse J - J. Exp. Bot. (2015)

Bottom Line: Thorough understanding of the enzymology of oil accumulation is critical to the goal of modifying oilseeds for improved vegetable oil production.This strong sensitivity of the BnDGAT1 isozymes to the relative concentrations of acyl-CoA substrates substantially explains the observed fatty acid composition of B. napus seed oil.Understanding these enzymes that are critical for triacylglycerol synthesis will facilitate genetic and biotechnological manipulations to improve this oilseed crop.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.

No MeSH data available.


Related in: MedlinePlus

Transcriptional profiling BnDGAT1. The transcripts from four candidate BnDGAT1 genes are shown at four stages of seed development. Data are represented as fragments per kilobase of transcript per million mapped reads (FPKM) = number of reads × (1000/gene length) × (1 million/total number of reads). (Refer to text for details.)
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Figure 3: Transcriptional profiling BnDGAT1. The transcripts from four candidate BnDGAT1 genes are shown at four stages of seed development. Data are represented as fragments per kilobase of transcript per million mapped reads (FPKM) = number of reads × (1000/gene length) × (1 million/total number of reads). (Refer to text for details.)

Mentions: Transcriptional profiles of developing B. napus seeds were analysed to determine expression of the BnDGAT1 genes through the course of seed development. Two genes, BnDGAT1-1 and BnDGAT1-2, exhibited moderate levels of transcript, ∼5 fragments per kilobase of transcript per million mapped reads (FPKM), early in seed development, after which the transcript for both rapidly increased 5- to 6-fold over the course of seed development, reaching 25−35 FPKM by 33−35 days after flowering (DAF) (Fig. 3). This transcript profile accords with the reported developmental profile of DGAT enzyme activity assayed in developing B. napus, when activity peaked at 35 DAF (Weselake et al., 1993). Expression of RNA for the other two isoforms, BnDGAT1-3 and BnDGAT1-4, was consistently much lower, starting near 2.5 FPKM for the first (20 DAF) interval and not exceeding 5 FPKM during the entire developmental course (Fig. 3).


Type 1 diacylglycerol acyltransferases of Brassica napus preferentially incorporate oleic acid into triacylglycerol.

Aznar-Moreno J, Denolf P, Van Audenhove K, De Bodt S, Engelen S, Fahy D, Wallis JG, Browse J - J. Exp. Bot. (2015)

Transcriptional profiling BnDGAT1. The transcripts from four candidate BnDGAT1 genes are shown at four stages of seed development. Data are represented as fragments per kilobase of transcript per million mapped reads (FPKM) = number of reads × (1000/gene length) × (1 million/total number of reads). (Refer to text for details.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588894&req=5

Figure 3: Transcriptional profiling BnDGAT1. The transcripts from four candidate BnDGAT1 genes are shown at four stages of seed development. Data are represented as fragments per kilobase of transcript per million mapped reads (FPKM) = number of reads × (1000/gene length) × (1 million/total number of reads). (Refer to text for details.)
Mentions: Transcriptional profiles of developing B. napus seeds were analysed to determine expression of the BnDGAT1 genes through the course of seed development. Two genes, BnDGAT1-1 and BnDGAT1-2, exhibited moderate levels of transcript, ∼5 fragments per kilobase of transcript per million mapped reads (FPKM), early in seed development, after which the transcript for both rapidly increased 5- to 6-fold over the course of seed development, reaching 25−35 FPKM by 33−35 days after flowering (DAF) (Fig. 3). This transcript profile accords with the reported developmental profile of DGAT enzyme activity assayed in developing B. napus, when activity peaked at 35 DAF (Weselake et al., 1993). Expression of RNA for the other two isoforms, BnDGAT1-3 and BnDGAT1-4, was consistently much lower, starting near 2.5 FPKM for the first (20 DAF) interval and not exceeding 5 FPKM during the entire developmental course (Fig. 3).

Bottom Line: Thorough understanding of the enzymology of oil accumulation is critical to the goal of modifying oilseeds for improved vegetable oil production.This strong sensitivity of the BnDGAT1 isozymes to the relative concentrations of acyl-CoA substrates substantially explains the observed fatty acid composition of B. napus seed oil.Understanding these enzymes that are critical for triacylglycerol synthesis will facilitate genetic and biotechnological manipulations to improve this oilseed crop.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.

No MeSH data available.


Related in: MedlinePlus