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Candidate gene selection and detailed morphological evaluations of fs8.1, a quantitative trait locus controlling tomato fruit shape.

Sun L, Rodriguez GR, Clevenger JP, Illa-Berenguer E, Lin J, Blakeslee JJ, Liu W, Fei Z, Wijeratne A, Meulia T, van der Knaap E - J. Exp. Bot. (2015)

Bottom Line: Of the 122 annotated genes found in the fs8.1 region, 51 were expressed during floral development and six were differentially expressed in anthesis-stage ovaries in fs8.1 and wild-type (WT) lines.This led to the identification of 158 single-nucleotide polymorphisms (SNPs) and five small indels in the fs8.1 interval, including 31 that could be associated with changes in gene expression or function.Fruit weight was also increased in fs8.1 compared with WT, which was predominantly attributed to the increased fruit length.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691, USA.

No MeSH data available.


Fine-mapping of fs8.1 to a 3.03Mb region. Fruit shape index is the ratio of maximal length to maximal width, and eight fruits per plant in each genotype were evaluated; ****P<0.001 by Student’s t-test; N, plant number. The positions of the centromere (black dot) and pericentromeric heterochromatin (grey shading) are according to Shearer et al. (2014).
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Figure 2: Fine-mapping of fs8.1 to a 3.03Mb region. Fruit shape index is the ratio of maximal length to maximal width, and eight fruits per plant in each genotype were evaluated; ****P<0.001 by Student’s t-test; N, plant number. The positions of the centromere (black dot) and pericentromeric heterochromatin (grey shading) are according to Shearer et al. (2014).

Mentions: Using the cultivated tomato and LA1589 genome sequences for additional marker development, we identified three recombinant plants in the fs8.1 region. Detailed investigations showed that plant 11S51-18, selfed progeny of plant 10S168#2–51, carried recombination breakpoints between markers 11EP239 at 48.9 and 12EP21 at 49.1Mb on one side and between markers 11EP245 at 51.8 and 11EP249 at 51.9Mb on the other side (Fig. 2). Progeny testing of this line showed a significant association of fruit shape with the allele at the locus (P<0.001). Thus, we concluded that fs8.1 was in the region between markers 11EP239 and 11EP249 with a physical distance of approximately 3.03Mb (Fig. 2). Based on the reference genome and chromosome structure, this region was located on a single scaffold (SGN: SL2.40sc04948) that contained 52 gaps of 100bp or larger (Supplementary Table S4, available at JXB online) and might harbour the border of heterochromatin to euchromatin (Shearer et al., 2014).


Candidate gene selection and detailed morphological evaluations of fs8.1, a quantitative trait locus controlling tomato fruit shape.

Sun L, Rodriguez GR, Clevenger JP, Illa-Berenguer E, Lin J, Blakeslee JJ, Liu W, Fei Z, Wijeratne A, Meulia T, van der Knaap E - J. Exp. Bot. (2015)

Fine-mapping of fs8.1 to a 3.03Mb region. Fruit shape index is the ratio of maximal length to maximal width, and eight fruits per plant in each genotype were evaluated; ****P<0.001 by Student’s t-test; N, plant number. The positions of the centromere (black dot) and pericentromeric heterochromatin (grey shading) are according to Shearer et al. (2014).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588892&req=5

Figure 2: Fine-mapping of fs8.1 to a 3.03Mb region. Fruit shape index is the ratio of maximal length to maximal width, and eight fruits per plant in each genotype were evaluated; ****P<0.001 by Student’s t-test; N, plant number. The positions of the centromere (black dot) and pericentromeric heterochromatin (grey shading) are according to Shearer et al. (2014).
Mentions: Using the cultivated tomato and LA1589 genome sequences for additional marker development, we identified three recombinant plants in the fs8.1 region. Detailed investigations showed that plant 11S51-18, selfed progeny of plant 10S168#2–51, carried recombination breakpoints between markers 11EP239 at 48.9 and 12EP21 at 49.1Mb on one side and between markers 11EP245 at 51.8 and 11EP249 at 51.9Mb on the other side (Fig. 2). Progeny testing of this line showed a significant association of fruit shape with the allele at the locus (P<0.001). Thus, we concluded that fs8.1 was in the region between markers 11EP239 and 11EP249 with a physical distance of approximately 3.03Mb (Fig. 2). Based on the reference genome and chromosome structure, this region was located on a single scaffold (SGN: SL2.40sc04948) that contained 52 gaps of 100bp or larger (Supplementary Table S4, available at JXB online) and might harbour the border of heterochromatin to euchromatin (Shearer et al., 2014).

Bottom Line: Of the 122 annotated genes found in the fs8.1 region, 51 were expressed during floral development and six were differentially expressed in anthesis-stage ovaries in fs8.1 and wild-type (WT) lines.This led to the identification of 158 single-nucleotide polymorphisms (SNPs) and five small indels in the fs8.1 interval, including 31 that could be associated with changes in gene expression or function.Fruit weight was also increased in fs8.1 compared with WT, which was predominantly attributed to the increased fruit length.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691, USA.

No MeSH data available.