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Candidate gene selection and detailed morphological evaluations of fs8.1, a quantitative trait locus controlling tomato fruit shape.

Sun L, Rodriguez GR, Clevenger JP, Illa-Berenguer E, Lin J, Blakeslee JJ, Liu W, Fei Z, Wijeratne A, Meulia T, van der Knaap E - J. Exp. Bot. (2015)

Bottom Line: Of the 122 annotated genes found in the fs8.1 region, 51 were expressed during floral development and six were differentially expressed in anthesis-stage ovaries in fs8.1 and wild-type (WT) lines.This led to the identification of 158 single-nucleotide polymorphisms (SNPs) and five small indels in the fs8.1 interval, including 31 that could be associated with changes in gene expression or function.Fruit weight was also increased in fs8.1 compared with WT, which was predominantly attributed to the increased fruit length.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691, USA.

No MeSH data available.


Morphological and histological analyses of reproductive organs in fs8.1 NILs. (A) Fruit shape index measurement using Tomato Analyzer version 3.0 (TA). Fruit shape index=L/W. (B) Fruit width widest position measurement using Tomato Analyzer. Width widest position=y/L. (C) Fruit structure measurement using Tomato Analyzer. Pericarp thickness=(h1+h2+w1+w2)/4; pericarp area is defined as the area between the yellow and blue lines; the placenta is indicated in green; the septum is indicated in purple. (D) Floral organ shape analysis. Ovary shape index=L/W. (E) Ovary wall and columella cell number and size measurements in a proximal–distal section of an anthesis-stage ovary. CL, columella length; ‘a’ to ‘f’ indicates where the ovary wall thickness and cell layer were measured. (F) Ovary wall cell number measurement in a mediolateral section of an anthesis-stage ovary. ‘1’ to ‘4’ and pink areas indicate where the cell number and size were measured. (G) Cell layer and large-cell size measurements in a pericarp section. The pericarp cell layer was measured along the black lines in the abaxial–adaxial direction. Large-cell size was measured in areas I and II by averaging the areas of the six biggest cells. (H) Average cell size measurement in a pericarp section. Average cell size was measured in two boxes (α and β) at the position of one-third and two-thirds of pericarp thickness; average cell size=area(α+β)/total cell number(α+β).
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Figure 1: Morphological and histological analyses of reproductive organs in fs8.1 NILs. (A) Fruit shape index measurement using Tomato Analyzer version 3.0 (TA). Fruit shape index=L/W. (B) Fruit width widest position measurement using Tomato Analyzer. Width widest position=y/L. (C) Fruit structure measurement using Tomato Analyzer. Pericarp thickness=(h1+h2+w1+w2)/4; pericarp area is defined as the area between the yellow and blue lines; the placenta is indicated in green; the septum is indicated in purple. (D) Floral organ shape analysis. Ovary shape index=L/W. (E) Ovary wall and columella cell number and size measurements in a proximal–distal section of an anthesis-stage ovary. CL, columella length; ‘a’ to ‘f’ indicates where the ovary wall thickness and cell layer were measured. (F) Ovary wall cell number measurement in a mediolateral section of an anthesis-stage ovary. ‘1’ to ‘4’ and pink areas indicate where the cell number and size were measured. (G) Cell layer and large-cell size measurements in a pericarp section. The pericarp cell layer was measured along the black lines in the abaxial–adaxial direction. Large-cell size was measured in areas I and II by averaging the areas of the six biggest cells. (H) Average cell size measurement in a pericarp section. Average cell size was measured in two boxes (α and β) at the position of one-third and two-thirds of pericarp thickness; average cell size=area(α+β)/total cell number(α+β).

Mentions: Fully mature tomato fruits collected from families 13S140, 13S117, and 13S118 were cut along the proximal–distal axis and scanned (Fig. 1A, B). The images were opened in Tomato Analyzer version 3.0 (Rodríguez et al., 2010) and morphology parameters were analysed according to the user manual (http://oardc.osu.edu/vanderknaap/tomato_analyzer.php). Floral organs were collected from the 13S117, 13S118, and 13S140 families at anthesis and flattened on 1.5% (w/v) agarose before being scanned (Fig. 1D). Floral organ shapes were evaluated using ImageJ (http://rsbweb.nih.gov/ij/).


Candidate gene selection and detailed morphological evaluations of fs8.1, a quantitative trait locus controlling tomato fruit shape.

Sun L, Rodriguez GR, Clevenger JP, Illa-Berenguer E, Lin J, Blakeslee JJ, Liu W, Fei Z, Wijeratne A, Meulia T, van der Knaap E - J. Exp. Bot. (2015)

Morphological and histological analyses of reproductive organs in fs8.1 NILs. (A) Fruit shape index measurement using Tomato Analyzer version 3.0 (TA). Fruit shape index=L/W. (B) Fruit width widest position measurement using Tomato Analyzer. Width widest position=y/L. (C) Fruit structure measurement using Tomato Analyzer. Pericarp thickness=(h1+h2+w1+w2)/4; pericarp area is defined as the area between the yellow and blue lines; the placenta is indicated in green; the septum is indicated in purple. (D) Floral organ shape analysis. Ovary shape index=L/W. (E) Ovary wall and columella cell number and size measurements in a proximal–distal section of an anthesis-stage ovary. CL, columella length; ‘a’ to ‘f’ indicates where the ovary wall thickness and cell layer were measured. (F) Ovary wall cell number measurement in a mediolateral section of an anthesis-stage ovary. ‘1’ to ‘4’ and pink areas indicate where the cell number and size were measured. (G) Cell layer and large-cell size measurements in a pericarp section. The pericarp cell layer was measured along the black lines in the abaxial–adaxial direction. Large-cell size was measured in areas I and II by averaging the areas of the six biggest cells. (H) Average cell size measurement in a pericarp section. Average cell size was measured in two boxes (α and β) at the position of one-third and two-thirds of pericarp thickness; average cell size=area(α+β)/total cell number(α+β).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 1: Morphological and histological analyses of reproductive organs in fs8.1 NILs. (A) Fruit shape index measurement using Tomato Analyzer version 3.0 (TA). Fruit shape index=L/W. (B) Fruit width widest position measurement using Tomato Analyzer. Width widest position=y/L. (C) Fruit structure measurement using Tomato Analyzer. Pericarp thickness=(h1+h2+w1+w2)/4; pericarp area is defined as the area between the yellow and blue lines; the placenta is indicated in green; the septum is indicated in purple. (D) Floral organ shape analysis. Ovary shape index=L/W. (E) Ovary wall and columella cell number and size measurements in a proximal–distal section of an anthesis-stage ovary. CL, columella length; ‘a’ to ‘f’ indicates where the ovary wall thickness and cell layer were measured. (F) Ovary wall cell number measurement in a mediolateral section of an anthesis-stage ovary. ‘1’ to ‘4’ and pink areas indicate where the cell number and size were measured. (G) Cell layer and large-cell size measurements in a pericarp section. The pericarp cell layer was measured along the black lines in the abaxial–adaxial direction. Large-cell size was measured in areas I and II by averaging the areas of the six biggest cells. (H) Average cell size measurement in a pericarp section. Average cell size was measured in two boxes (α and β) at the position of one-third and two-thirds of pericarp thickness; average cell size=area(α+β)/total cell number(α+β).
Mentions: Fully mature tomato fruits collected from families 13S140, 13S117, and 13S118 were cut along the proximal–distal axis and scanned (Fig. 1A, B). The images were opened in Tomato Analyzer version 3.0 (Rodríguez et al., 2010) and morphology parameters were analysed according to the user manual (http://oardc.osu.edu/vanderknaap/tomato_analyzer.php). Floral organs were collected from the 13S117, 13S118, and 13S140 families at anthesis and flattened on 1.5% (w/v) agarose before being scanned (Fig. 1D). Floral organ shapes were evaluated using ImageJ (http://rsbweb.nih.gov/ij/).

Bottom Line: Of the 122 annotated genes found in the fs8.1 region, 51 were expressed during floral development and six were differentially expressed in anthesis-stage ovaries in fs8.1 and wild-type (WT) lines.This led to the identification of 158 single-nucleotide polymorphisms (SNPs) and five small indels in the fs8.1 interval, including 31 that could be associated with changes in gene expression or function.Fruit weight was also increased in fs8.1 compared with WT, which was predominantly attributed to the increased fruit length.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691, USA.

No MeSH data available.