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G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus

Expression of SxCobL4and SxFLA12 genes in transverse sections probed with antisense probes. (A) COBRA gene expression after 1 week of tipping is shown by the purple staining on the TW side of the stem. (B) A 2-week tipped stem showing that SxFLA12 gene expression occurs on the TW side of the stem. (C) Lack of purple colour in a control upright stem indicates no expression of the COBRA gene after 1 weeks of growth. (D) 1-week inclined stem shows strong expression of the COBRA gene in the G-fibres of the TW side. (E, F) After 2 and 4 weeks of tipping, respectively, strong expression of COBRA gene is shown in the G-fibres. (G–I) No COBRA gene expression is detected in OW of the same stems in D, E, and F, respectively. Bars: A–C: 100 µm, D–I: 100 µm.
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Figure 8: Expression of SxCobL4and SxFLA12 genes in transverse sections probed with antisense probes. (A) COBRA gene expression after 1 week of tipping is shown by the purple staining on the TW side of the stem. (B) A 2-week tipped stem showing that SxFLA12 gene expression occurs on the TW side of the stem. (C) Lack of purple colour in a control upright stem indicates no expression of the COBRA gene after 1 weeks of growth. (D) 1-week inclined stem shows strong expression of the COBRA gene in the G-fibres of the TW side. (E, F) After 2 and 4 weeks of tipping, respectively, strong expression of COBRA gene is shown in the G-fibres. (G–I) No COBRA gene expression is detected in OW of the same stems in D, E, and F, respectively. Bars: A–C: 100 µm, D–I: 100 µm.

Mentions: Both SxCOBL4 and SxFLA12 transcripts were strongly expressed in G-fibres of TW (Fig. 8A, B, Supplementary Fig. S3) but absent in NW of straight control stems (Fig. 8C). Expression started after 1 week of inclined growth and increased after 2 and 4 weeks of inclined growth in newly formed G-fibres as well as in mature fibres (Fig. 8). By contrast, no signal was detected in OW (Figure 8A, B, G, H, I) and in NW of control stems (Fig. 8C). Light staining was occasionally observed in control stems, probably due to the development of some G-fibres resulting from natural movement of the stem (Supplementary Fig. S3). No hybridization was detected using the sense probes in both NW and TW (Supplementary Fig. S3) and control straight stems.


G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Expression of SxCobL4and SxFLA12 genes in transverse sections probed with antisense probes. (A) COBRA gene expression after 1 week of tipping is shown by the purple staining on the TW side of the stem. (B) A 2-week tipped stem showing that SxFLA12 gene expression occurs on the TW side of the stem. (C) Lack of purple colour in a control upright stem indicates no expression of the COBRA gene after 1 weeks of growth. (D) 1-week inclined stem shows strong expression of the COBRA gene in the G-fibres of the TW side. (E, F) After 2 and 4 weeks of tipping, respectively, strong expression of COBRA gene is shown in the G-fibres. (G–I) No COBRA gene expression is detected in OW of the same stems in D, E, and F, respectively. Bars: A–C: 100 µm, D–I: 100 µm.
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Figure 8: Expression of SxCobL4and SxFLA12 genes in transverse sections probed with antisense probes. (A) COBRA gene expression after 1 week of tipping is shown by the purple staining on the TW side of the stem. (B) A 2-week tipped stem showing that SxFLA12 gene expression occurs on the TW side of the stem. (C) Lack of purple colour in a control upright stem indicates no expression of the COBRA gene after 1 weeks of growth. (D) 1-week inclined stem shows strong expression of the COBRA gene in the G-fibres of the TW side. (E, F) After 2 and 4 weeks of tipping, respectively, strong expression of COBRA gene is shown in the G-fibres. (G–I) No COBRA gene expression is detected in OW of the same stems in D, E, and F, respectively. Bars: A–C: 100 µm, D–I: 100 µm.
Mentions: Both SxCOBL4 and SxFLA12 transcripts were strongly expressed in G-fibres of TW (Fig. 8A, B, Supplementary Fig. S3) but absent in NW of straight control stems (Fig. 8C). Expression started after 1 week of inclined growth and increased after 2 and 4 weeks of inclined growth in newly formed G-fibres as well as in mature fibres (Fig. 8). By contrast, no signal was detected in OW (Figure 8A, B, G, H, I) and in NW of control stems (Fig. 8C). Light staining was occasionally observed in control stems, probably due to the development of some G-fibres resulting from natural movement of the stem (Supplementary Fig. S3). No hybridization was detected using the sense probes in both NW and TW (Supplementary Fig. S3) and control straight stems.

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus