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G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus

Transverse 100 µm thick unembedded stem sections stained with Calcofluor white showing the distribution of cellulose. (A) Stem after 1 week of TW induction. (B) Stem after 2 weeks of TW induction. (C) TW developing G-fibres after 4 weeks of inclined growth. (D) TW maturing G-fibres after 4 weeks of inclined growth. (E) TW mature G-fibres after 4 weeks of inclined growth. (F) OW maturing normal fibres after 4 weeks of inclined growth. ca, cambium; rp, ray parenchyma; v, xylem vessel. Bars: A, B = 500 µm; B, C, D, F = 10 µm.
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Figure 6: Transverse 100 µm thick unembedded stem sections stained with Calcofluor white showing the distribution of cellulose. (A) Stem after 1 week of TW induction. (B) Stem after 2 weeks of TW induction. (C) TW developing G-fibres after 4 weeks of inclined growth. (D) TW maturing G-fibres after 4 weeks of inclined growth. (E) TW mature G-fibres after 4 weeks of inclined growth. (F) OW maturing normal fibres after 4 weeks of inclined growth. ca, cambium; rp, ray parenchyma; v, xylem vessel. Bars: A, B = 500 µm; B, C, D, F = 10 µm.

Mentions: Strong fluorescence was observed with Calcofluor white in the G-layers of TW after the first week of inclined growth (Fig. 6A). Fluorescence extended into the G-layers in conjunction with G-fibre maturation (Fig. 6B–E). In OW, fibres were not clearly stained because of the lack of the very thick G-layer (Fig. 6F).


G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Transverse 100 µm thick unembedded stem sections stained with Calcofluor white showing the distribution of cellulose. (A) Stem after 1 week of TW induction. (B) Stem after 2 weeks of TW induction. (C) TW developing G-fibres after 4 weeks of inclined growth. (D) TW maturing G-fibres after 4 weeks of inclined growth. (E) TW mature G-fibres after 4 weeks of inclined growth. (F) OW maturing normal fibres after 4 weeks of inclined growth. ca, cambium; rp, ray parenchyma; v, xylem vessel. Bars: A, B = 500 µm; B, C, D, F = 10 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4588891&req=5

Figure 6: Transverse 100 µm thick unembedded stem sections stained with Calcofluor white showing the distribution of cellulose. (A) Stem after 1 week of TW induction. (B) Stem after 2 weeks of TW induction. (C) TW developing G-fibres after 4 weeks of inclined growth. (D) TW maturing G-fibres after 4 weeks of inclined growth. (E) TW mature G-fibres after 4 weeks of inclined growth. (F) OW maturing normal fibres after 4 weeks of inclined growth. ca, cambium; rp, ray parenchyma; v, xylem vessel. Bars: A, B = 500 µm; B, C, D, F = 10 µm.
Mentions: Strong fluorescence was observed with Calcofluor white in the G-layers of TW after the first week of inclined growth (Fig. 6A). Fluorescence extended into the G-layers in conjunction with G-fibre maturation (Fig. 6B–E). In OW, fibres were not clearly stained because of the lack of the very thick G-layer (Fig. 6F).

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus