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G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus

Mannan (LM21 antibody) distribution in TW and OW after 4 weeks of inclined growth. (A) 1 µm resin section of TW side showing strong binding of LM21 in the maturing and mature G-fibres. The numbers in the squares indicate similar areas to those shown in detail in B–D. (B) 100 µm unembedded section of developing G-fibres of area 1 showing a thin layer of labelling in the SCW/G-layer (arrow). (C) 100 µm unembedded section of more mature fibres showing labelling at the SCW/G-layer interface. (D) 100 µm unembedded section of very thick-walled mature G-fibres showing binding of the LM21 antibody in the SCW and also some more diffuse labelling in the G-layer. (E) 1 µm resin section of OW side showing very weak binding to fibre cell walls. (F) Detail of fibres in (E) showing light labelling of cell walls, possibly S1 layer of the SCW. Bars: A, E, 100 µm; B, C, D, F, 10 µm.
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Figure 5: Mannan (LM21 antibody) distribution in TW and OW after 4 weeks of inclined growth. (A) 1 µm resin section of TW side showing strong binding of LM21 in the maturing and mature G-fibres. The numbers in the squares indicate similar areas to those shown in detail in B–D. (B) 100 µm unembedded section of developing G-fibres of area 1 showing a thin layer of labelling in the SCW/G-layer (arrow). (C) 100 µm unembedded section of more mature fibres showing labelling at the SCW/G-layer interface. (D) 100 µm unembedded section of very thick-walled mature G-fibres showing binding of the LM21 antibody in the SCW and also some more diffuse labelling in the G-layer. (E) 1 µm resin section of OW side showing very weak binding to fibre cell walls. (F) Detail of fibres in (E) showing light labelling of cell walls, possibly S1 layer of the SCW. Bars: A, E, 100 µm; B, C, D, F, 10 µm.

Mentions: Immunofluorescence showed that the LM21 antibody was mainly bound to G-fibre cell walls of TW (Fig. 5 A), with labelling in the interface between the SCW and the G-layer in the developing and maturing fibres (Fig. 5B, C). In fully mature cells the G-layer itself was also labelled; however, the signal was weaker (Fig. 5D). At low magnification, no significant labelling could be seen (Fig. 5E) in OW, and at higher magnification only weak labelling was detected in the region that may be interpreted as being the S1 layer of the SCWs (Fig. 5F).


G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Mannan (LM21 antibody) distribution in TW and OW after 4 weeks of inclined growth. (A) 1 µm resin section of TW side showing strong binding of LM21 in the maturing and mature G-fibres. The numbers in the squares indicate similar areas to those shown in detail in B–D. (B) 100 µm unembedded section of developing G-fibres of area 1 showing a thin layer of labelling in the SCW/G-layer (arrow). (C) 100 µm unembedded section of more mature fibres showing labelling at the SCW/G-layer interface. (D) 100 µm unembedded section of very thick-walled mature G-fibres showing binding of the LM21 antibody in the SCW and also some more diffuse labelling in the G-layer. (E) 1 µm resin section of OW side showing very weak binding to fibre cell walls. (F) Detail of fibres in (E) showing light labelling of cell walls, possibly S1 layer of the SCW. Bars: A, E, 100 µm; B, C, D, F, 10 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588891&req=5

Figure 5: Mannan (LM21 antibody) distribution in TW and OW after 4 weeks of inclined growth. (A) 1 µm resin section of TW side showing strong binding of LM21 in the maturing and mature G-fibres. The numbers in the squares indicate similar areas to those shown in detail in B–D. (B) 100 µm unembedded section of developing G-fibres of area 1 showing a thin layer of labelling in the SCW/G-layer (arrow). (C) 100 µm unembedded section of more mature fibres showing labelling at the SCW/G-layer interface. (D) 100 µm unembedded section of very thick-walled mature G-fibres showing binding of the LM21 antibody in the SCW and also some more diffuse labelling in the G-layer. (E) 1 µm resin section of OW side showing very weak binding to fibre cell walls. (F) Detail of fibres in (E) showing light labelling of cell walls, possibly S1 layer of the SCW. Bars: A, E, 100 µm; B, C, D, F, 10 µm.
Mentions: Immunofluorescence showed that the LM21 antibody was mainly bound to G-fibre cell walls of TW (Fig. 5 A), with labelling in the interface between the SCW and the G-layer in the developing and maturing fibres (Fig. 5B, C). In fully mature cells the G-layer itself was also labelled; however, the signal was weaker (Fig. 5D). At low magnification, no significant labelling could be seen (Fig. 5E) in OW, and at higher magnification only weak labelling was detected in the region that may be interpreted as being the S1 layer of the SCWs (Fig. 5F).

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus