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G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus

Xylan (LM10 antibody) distribution in TW and OW after 4 weeks of tipping. (A) TW side. Numbers indicate the areas shown in detail in B, C and D. (B) Detail of area 1. Differentiating xylem. (C) Area 2. Maturing xylem. The G-layer is not labelled (arrow). Antibody labeling is only present in secondary cell walls of G-fibres, vessels and ray parenchyma. (D) Area 3. Mature xylem. Normal fibres have thicker secondary cell walls than G-fibres. (E) OW of same section shows strong labelling of all fibre SCWs. (F) and (G) show detail of areas 1 and 2 in (E). All fibre SCWs are strongly labelled. (v) xylem vessel, (f) fibre, (rp) ray parenchyma. Bars: A, E = 100 µm; B, C, D, F, G = 20 µm.
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Figure 4: Xylan (LM10 antibody) distribution in TW and OW after 4 weeks of tipping. (A) TW side. Numbers indicate the areas shown in detail in B, C and D. (B) Detail of area 1. Differentiating xylem. (C) Area 2. Maturing xylem. The G-layer is not labelled (arrow). Antibody labeling is only present in secondary cell walls of G-fibres, vessels and ray parenchyma. (D) Area 3. Mature xylem. Normal fibres have thicker secondary cell walls than G-fibres. (E) OW of same section shows strong labelling of all fibre SCWs. (F) and (G) show detail of areas 1 and 2 in (E). All fibre SCWs are strongly labelled. (v) xylem vessel, (f) fibre, (rp) ray parenchyma. Bars: A, E = 100 µm; B, C, D, F, G = 20 µm.

Mentions: Xylan was detected with the LM10 antibody in all SCWs in developing and mature xylem in TW, OW, and NW. The G-layer itself was not labelled, resulting in weaker fluorescence on the TW side (Fig. 4A, B, C). In OW, fluorescence was much stronger due to thicker SCWs (Fig. 4E, F, G). TEM confirmed these observations and showed labelling to be clearly confined to the SCWs (Fig. 7L, M, N).


G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Xylan (LM10 antibody) distribution in TW and OW after 4 weeks of tipping. (A) TW side. Numbers indicate the areas shown in detail in B, C and D. (B) Detail of area 1. Differentiating xylem. (C) Area 2. Maturing xylem. The G-layer is not labelled (arrow). Antibody labeling is only present in secondary cell walls of G-fibres, vessels and ray parenchyma. (D) Area 3. Mature xylem. Normal fibres have thicker secondary cell walls than G-fibres. (E) OW of same section shows strong labelling of all fibre SCWs. (F) and (G) show detail of areas 1 and 2 in (E). All fibre SCWs are strongly labelled. (v) xylem vessel, (f) fibre, (rp) ray parenchyma. Bars: A, E = 100 µm; B, C, D, F, G = 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588891&req=5

Figure 4: Xylan (LM10 antibody) distribution in TW and OW after 4 weeks of tipping. (A) TW side. Numbers indicate the areas shown in detail in B, C and D. (B) Detail of area 1. Differentiating xylem. (C) Area 2. Maturing xylem. The G-layer is not labelled (arrow). Antibody labeling is only present in secondary cell walls of G-fibres, vessels and ray parenchyma. (D) Area 3. Mature xylem. Normal fibres have thicker secondary cell walls than G-fibres. (E) OW of same section shows strong labelling of all fibre SCWs. (F) and (G) show detail of areas 1 and 2 in (E). All fibre SCWs are strongly labelled. (v) xylem vessel, (f) fibre, (rp) ray parenchyma. Bars: A, E = 100 µm; B, C, D, F, G = 20 µm.
Mentions: Xylan was detected with the LM10 antibody in all SCWs in developing and mature xylem in TW, OW, and NW. The G-layer itself was not labelled, resulting in weaker fluorescence on the TW side (Fig. 4A, B, C). In OW, fluorescence was much stronger due to thicker SCWs (Fig. 4E, F, G). TEM confirmed these observations and showed labelling to be clearly confined to the SCWs (Fig. 7L, M, N).

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus