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G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus

K8-428 willow plants. (A) Control upright plants. (B) Inclined plants for TW induction after 1 week of treatment. Bars: 10cm.
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Figure 1: K8-428 willow plants. (A) Control upright plants. (B) Inclined plants for TW induction after 1 week of treatment. Bars: 10cm.

Mentions: Plant material was grown under similar conditions to those described by Brereton et al. (2012). Stem cuttings measuring approximately 20cm in length × 10mm in diameter and containing an average of three axillary buds from K8-428 genotype <{Salix viminalis ‘Astrid’ × [S. viminalis ‘Astrid’ × (S. schwerinii × S. viminalis SW930984)] S3} × {S. viminalis ‘Astrid’ × [S. viminalis ‘Astrid’ × (S. schwerinii × S. viminalis SW930984)] R13}> were grown in Rothamsted standard compost mix in a glasshouse under a 16h day length regime. Two experiments were carried out. In Experiment I, the willow cuttings were grown for 4 weeks, after which TW was induced by inclining the stems to a 45° angle (Fig. 1). Samples were harvested from three replicate plants for fixation from the stem mid-point after 1 or 2 weeks of induction. As the diameter and hardness of the stems developed very quickly, in Experiment II, cuttings were grown for 2 weeks only prior to TW induction in order to facilitate their preparation for microscopy. Samples from this second experiment were collected after 4 weeks of induction. Control stems in both experiments were kept in an upright position. The growing tips were regularly tied to a supporting cane to maintain the correct orientation. In situ hybridization and immunolabelling results were found to be consistent between the two experiments.


G-fibre cell wall development in willow stems during tension wood induction.

Gritsch C, Wan Y, Mitchell RA, Shewry PR, Hanley SJ, Karp A - J. Exp. Bot. (2015)

K8-428 willow plants. (A) Control upright plants. (B) Inclined plants for TW induction after 1 week of treatment. Bars: 10cm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588891&req=5

Figure 1: K8-428 willow plants. (A) Control upright plants. (B) Inclined plants for TW induction after 1 week of treatment. Bars: 10cm.
Mentions: Plant material was grown under similar conditions to those described by Brereton et al. (2012). Stem cuttings measuring approximately 20cm in length × 10mm in diameter and containing an average of three axillary buds from K8-428 genotype <{Salix viminalis ‘Astrid’ × [S. viminalis ‘Astrid’ × (S. schwerinii × S. viminalis SW930984)] S3} × {S. viminalis ‘Astrid’ × [S. viminalis ‘Astrid’ × (S. schwerinii × S. viminalis SW930984)] R13}> were grown in Rothamsted standard compost mix in a glasshouse under a 16h day length regime. Two experiments were carried out. In Experiment I, the willow cuttings were grown for 4 weeks, after which TW was induced by inclining the stems to a 45° angle (Fig. 1). Samples were harvested from three replicate plants for fixation from the stem mid-point after 1 or 2 weeks of induction. As the diameter and hardness of the stems developed very quickly, in Experiment II, cuttings were grown for 2 weeks only prior to TW induction in order to facilitate their preparation for microscopy. Samples from this second experiment were collected after 4 weeks of induction. Control stems in both experiments were kept in an upright position. The growing tips were regularly tied to a supporting cane to maintain the correct orientation. In situ hybridization and immunolabelling results were found to be consistent between the two experiments.

Bottom Line: In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization.Deposition of the non-cellulosic polysaccharides (1-4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself.SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance.

View Article: PubMed Central - PubMed

Affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.

No MeSH data available.


Related in: MedlinePlus