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An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.

Yao X, Li J, Liu J, Liu K - J. Exp. Bot. (2015)

Bottom Line: High expression levels of RRL were found in germinating seeds and developing seedlings, as revealed by β-glucuronidase (GUS) staining of ProRRL-GUS transgenic lines.The analyses of the structure and function of mitochondria in the knockout rrl mutant showed that the disruption of RRL causes extensively internally vacuolated mitochondria and reduced ABA-stimulated reactive oxygen species (ROS) production.Furthermore, the results revealed that ABI4 is a downstream regulatory factor in RRL-mediated ABA signalling in seed germination and seedling growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.


Related in: MedlinePlus

Responses to glucose of the rrl knockout mutant, the OE-4 overexpression line, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid. Glucose hypersensitivity of the RRL overexpression line OE-4 (4–6% glucose) and glucose insensitivity of the rrl knockout mutant, abi4-1 mutant, abi4-1 rrl double mutant, and abi4-1 OE-4 hybrid (4–6% glucose) in early seedling development. Sor, sorbitol osmotic control; Glc, glucose. Scale bar=0.5cm. (This figure is available in colour at JXB online.)
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Figure 8: Responses to glucose of the rrl knockout mutant, the OE-4 overexpression line, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid. Glucose hypersensitivity of the RRL overexpression line OE-4 (4–6% glucose) and glucose insensitivity of the rrl knockout mutant, abi4-1 mutant, abi4-1 rrl double mutant, and abi4-1 OE-4 hybrid (4–6% glucose) in early seedling development. Sor, sorbitol osmotic control; Glc, glucose. Scale bar=0.5cm. (This figure is available in colour at JXB online.)

Mentions: Previous findings have revealed that ABI4 is involved in the ABA-mediated glucose response. Early seedling development is inhibited in the wild type in response to sugars. However, the abi4 mutant displays expanded green cotyledons and true leaves on 6% glucose (Arenas-Huertero et al., 2000; Huijse, et al., 2000). Thus the responses of the rrl mutant, the OE-4 overexpression line, the abi4-1 mutant, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid to glucose were next tested. The results showed that the rrl mutant, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid exhibited the same reduced sensitivity to sugar as the abi4-1 mutant on MS medium supplemented with 4% and 6% glucose. In contrast, the OE-4 overexpression line displayed increased sensitivity to 4% and 6% glucose compared with Col-0 (Fig. 8). Sorbitol at 4% and 6% was used as the osmotic control in this experiment. These results suggest that disruption of RRL renders seedlings sugar insensitive and RRL may participate in ABI4-mediated sugar signalling.


An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.

Yao X, Li J, Liu J, Liu K - J. Exp. Bot. (2015)

Responses to glucose of the rrl knockout mutant, the OE-4 overexpression line, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid. Glucose hypersensitivity of the RRL overexpression line OE-4 (4–6% glucose) and glucose insensitivity of the rrl knockout mutant, abi4-1 mutant, abi4-1 rrl double mutant, and abi4-1 OE-4 hybrid (4–6% glucose) in early seedling development. Sor, sorbitol osmotic control; Glc, glucose. Scale bar=0.5cm. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 8: Responses to glucose of the rrl knockout mutant, the OE-4 overexpression line, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid. Glucose hypersensitivity of the RRL overexpression line OE-4 (4–6% glucose) and glucose insensitivity of the rrl knockout mutant, abi4-1 mutant, abi4-1 rrl double mutant, and abi4-1 OE-4 hybrid (4–6% glucose) in early seedling development. Sor, sorbitol osmotic control; Glc, glucose. Scale bar=0.5cm. (This figure is available in colour at JXB online.)
Mentions: Previous findings have revealed that ABI4 is involved in the ABA-mediated glucose response. Early seedling development is inhibited in the wild type in response to sugars. However, the abi4 mutant displays expanded green cotyledons and true leaves on 6% glucose (Arenas-Huertero et al., 2000; Huijse, et al., 2000). Thus the responses of the rrl mutant, the OE-4 overexpression line, the abi4-1 mutant, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid to glucose were next tested. The results showed that the rrl mutant, the abi4-1 rrl double mutant, and the abi4-1 OE-4 hybrid exhibited the same reduced sensitivity to sugar as the abi4-1 mutant on MS medium supplemented with 4% and 6% glucose. In contrast, the OE-4 overexpression line displayed increased sensitivity to 4% and 6% glucose compared with Col-0 (Fig. 8). Sorbitol at 4% and 6% was used as the osmotic control in this experiment. These results suggest that disruption of RRL renders seedlings sugar insensitive and RRL may participate in ABI4-mediated sugar signalling.

Bottom Line: High expression levels of RRL were found in germinating seeds and developing seedlings, as revealed by β-glucuronidase (GUS) staining of ProRRL-GUS transgenic lines.The analyses of the structure and function of mitochondria in the knockout rrl mutant showed that the disruption of RRL causes extensively internally vacuolated mitochondria and reduced ABA-stimulated reactive oxygen species (ROS) production.Furthermore, the results revealed that ABI4 is a downstream regulatory factor in RRL-mediated ABA signalling in seed germination and seedling growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.


Related in: MedlinePlus