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An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.

Yao X, Li J, Liu J, Liu K - J. Exp. Bot. (2015)

Bottom Line: High expression levels of RRL were found in germinating seeds and developing seedlings, as revealed by β-glucuronidase (GUS) staining of ProRRL-GUS transgenic lines.The analyses of the structure and function of mitochondria in the knockout rrl mutant showed that the disruption of RRL causes extensively internally vacuolated mitochondria and reduced ABA-stimulated reactive oxygen species (ROS) production.Furthermore, the results revealed that ABI4 is a downstream regulatory factor in RRL-mediated ABA signalling in seed germination and seedling growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.


The expression pattern of RRL. (A) Semi-quantitative analysis of RRL gene expression in various tissues. ACTIN2 was used as a loading control. (B–J) RRL promoter-driven GUS expression in germinating seeds and developing seedlings (B–D), 4-day-old seedlings (E), rosette leaves (F), flowers (G, H), and siliques (I, J). Scale bars=0.1mm (B, C, D, E, J); 1mm (F, G, I). (H) Magnification of the areas outlined in (G). Scale bar=0.1mm (H). (This figure is available in colour at JXB online.)
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Figure 4: The expression pattern of RRL. (A) Semi-quantitative analysis of RRL gene expression in various tissues. ACTIN2 was used as a loading control. (B–J) RRL promoter-driven GUS expression in germinating seeds and developing seedlings (B–D), 4-day-old seedlings (E), rosette leaves (F), flowers (G, H), and siliques (I, J). Scale bars=0.1mm (B, C, D, E, J); 1mm (F, G, I). (H) Magnification of the areas outlined in (G). Scale bar=0.1mm (H). (This figure is available in colour at JXB online.)

Mentions: In order to study the specific function of RRL in seed germination and seedling growth, the expression pattern of the RRL gene was next investigated. Semi-quantitative RT-PCR was performed to assess RRL gene expression in various tissues including 7-day-old seedlings, roots, rosette leaves, stem, flowers, and siliques. RRL was expressed in various tissues (Fig. 4A). In the more detailed GUS staining analyses, 10 independent transgenic Arabidopsis lines harbouring the ProRRL–GUS construct were obtained and homozygote T3 lines were used for further analyses. Strong GUS staining was detected in germinating seeds and the early developing seedlings (24–96h after seed germination; Fig. 4B–E). In contrast, very weak GUS signal was observed in vascular tissues of rosette leaves (Fig. 4F). Moreover, GUS staining could also be detected in filaments, pollen in flowers (Fig. 4G, H), and false dissepiments and seed stalks in siliques (Fig. 4I, J). The high expression of RRL in germinating seeds and developing seedlings suggests a role for RRL in these developmental stages, consistent with the finding that RRL mediates ABA signalling in seed germination and seedling growth.


An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.

Yao X, Li J, Liu J, Liu K - J. Exp. Bot. (2015)

The expression pattern of RRL. (A) Semi-quantitative analysis of RRL gene expression in various tissues. ACTIN2 was used as a loading control. (B–J) RRL promoter-driven GUS expression in germinating seeds and developing seedlings (B–D), 4-day-old seedlings (E), rosette leaves (F), flowers (G, H), and siliques (I, J). Scale bars=0.1mm (B, C, D, E, J); 1mm (F, G, I). (H) Magnification of the areas outlined in (G). Scale bar=0.1mm (H). (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 4: The expression pattern of RRL. (A) Semi-quantitative analysis of RRL gene expression in various tissues. ACTIN2 was used as a loading control. (B–J) RRL promoter-driven GUS expression in germinating seeds and developing seedlings (B–D), 4-day-old seedlings (E), rosette leaves (F), flowers (G, H), and siliques (I, J). Scale bars=0.1mm (B, C, D, E, J); 1mm (F, G, I). (H) Magnification of the areas outlined in (G). Scale bar=0.1mm (H). (This figure is available in colour at JXB online.)
Mentions: In order to study the specific function of RRL in seed germination and seedling growth, the expression pattern of the RRL gene was next investigated. Semi-quantitative RT-PCR was performed to assess RRL gene expression in various tissues including 7-day-old seedlings, roots, rosette leaves, stem, flowers, and siliques. RRL was expressed in various tissues (Fig. 4A). In the more detailed GUS staining analyses, 10 independent transgenic Arabidopsis lines harbouring the ProRRL–GUS construct were obtained and homozygote T3 lines were used for further analyses. Strong GUS staining was detected in germinating seeds and the early developing seedlings (24–96h after seed germination; Fig. 4B–E). In contrast, very weak GUS signal was observed in vascular tissues of rosette leaves (Fig. 4F). Moreover, GUS staining could also be detected in filaments, pollen in flowers (Fig. 4G, H), and false dissepiments and seed stalks in siliques (Fig. 4I, J). The high expression of RRL in germinating seeds and developing seedlings suggests a role for RRL in these developmental stages, consistent with the finding that RRL mediates ABA signalling in seed germination and seedling growth.

Bottom Line: High expression levels of RRL were found in germinating seeds and developing seedlings, as revealed by β-glucuronidase (GUS) staining of ProRRL-GUS transgenic lines.The analyses of the structure and function of mitochondria in the knockout rrl mutant showed that the disruption of RRL causes extensively internally vacuolated mitochondria and reduced ABA-stimulated reactive oxygen species (ROS) production.Furthermore, the results revealed that ABI4 is a downstream regulatory factor in RRL-mediated ABA signalling in seed germination and seedling growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.