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High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.


Relative expression (2-ΔCt) of genes encoding enzymes involved in polyamine biosynthesis in nine cultivars of rice. (A) Relative gene expression after 48 DAS under control conditions. (B) Relative gene expression after 23 days (48 DAS) of HNT conditions. (C) Log2 fold changes in relative gene expression after 23 days (48 DAS) of HNT in comparison to control conditions. Data represent the means of one experiment with five replicates each. Cultivars are sorted from tolerant to sensitive (left to right).
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Figure 7: Relative expression (2-ΔCt) of genes encoding enzymes involved in polyamine biosynthesis in nine cultivars of rice. (A) Relative gene expression after 48 DAS under control conditions. (B) Relative gene expression after 23 days (48 DAS) of HNT conditions. (C) Log2 fold changes in relative gene expression after 23 days (48 DAS) of HNT in comparison to control conditions. Data represent the means of one experiment with five replicates each. Cultivars are sorted from tolerant to sensitive (left to right).

Mentions: ADC catalyses the first committed step of Put biosynthesis. Under control conditions, only the relative expression of ADC2 showed a tolerance-specific pattern, with higher levels in the tolerant and two intermediate than in the sensitive cultivars (Fig. 7A; Supplementary Table S6). However, ADC2 transcript levels were not significantly correlated with HNT sensitivity (Fig. 8, Supplementary Table S7). AIH showed a similar expression pattern but in this case, a significant negative correlation with HNT sensitivity was observed. Similar correlations were also found for CPA1, SPD/SPM1 and SPD/SPM2 (Figs 7A, 8). Transcript levels of all other genes showed no HNT sensitivity related patterns under control conditions.


High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Relative expression (2-ΔCt) of genes encoding enzymes involved in polyamine biosynthesis in nine cultivars of rice. (A) Relative gene expression after 48 DAS under control conditions. (B) Relative gene expression after 23 days (48 DAS) of HNT conditions. (C) Log2 fold changes in relative gene expression after 23 days (48 DAS) of HNT in comparison to control conditions. Data represent the means of one experiment with five replicates each. Cultivars are sorted from tolerant to sensitive (left to right).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588888&req=5

Figure 7: Relative expression (2-ΔCt) of genes encoding enzymes involved in polyamine biosynthesis in nine cultivars of rice. (A) Relative gene expression after 48 DAS under control conditions. (B) Relative gene expression after 23 days (48 DAS) of HNT conditions. (C) Log2 fold changes in relative gene expression after 23 days (48 DAS) of HNT in comparison to control conditions. Data represent the means of one experiment with five replicates each. Cultivars are sorted from tolerant to sensitive (left to right).
Mentions: ADC catalyses the first committed step of Put biosynthesis. Under control conditions, only the relative expression of ADC2 showed a tolerance-specific pattern, with higher levels in the tolerant and two intermediate than in the sensitive cultivars (Fig. 7A; Supplementary Table S6). However, ADC2 transcript levels were not significantly correlated with HNT sensitivity (Fig. 8, Supplementary Table S7). AIH showed a similar expression pattern but in this case, a significant negative correlation with HNT sensitivity was observed. Similar correlations were also found for CPA1, SPD/SPM1 and SPD/SPM2 (Figs 7A, 8). Transcript levels of all other genes showed no HNT sensitivity related patterns under control conditions.

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.