Limits...
High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.


Significant (  p<0.05) correlations of the polyamines Put, Spd and Spm with 75 other metabolites. (A) Significant correlations under control conditions. (B) Significant correlations under HNT conditions. Solid lines indicate positive, dashed line negative correlations.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588888&req=5

Figure 6: Significant (  p<0.05) correlations of the polyamines Put, Spd and Spm with 75 other metabolites. (A) Significant correlations under control conditions. (B) Significant correlations under HNT conditions. Solid lines indicate positive, dashed line negative correlations.

Mentions: Since the content of all three polyamines was significantly correlated with the HNT sensitivity of the cultivars, we wanted to further elucidate the connection of this pathway with the central metabolism. We therefore performed a Spearman’s rank order correlation analysis of polyamine pool sizes with those of all other measured metabolites. Significant correlations differed widely between control and HNT conditions, with six and 19 correlations, respectively (Fig. 6). While Spd was positively correlated with Put and Spm under both conditions, Put was additionally correlated with Spm under HNT conditions. In addition, only the correlations of Spm with glyceric acid 2-P and glyceric acid 3-P were observed under both conditions and these metabolites were not correlated with HNT sensitivity. However, eight correlations of polyamines with amino acids or organic acids, whose levels were correlated with HNT sensitivity, were specific to HNT conditions. Further, a negative correlation between Spm and myo-inositol pool sizes was observed under HNT, where also a negative correlation of this sugar alcohol with HNT sensitivity was observed.


High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Significant (  p<0.05) correlations of the polyamines Put, Spd and Spm with 75 other metabolites. (A) Significant correlations under control conditions. (B) Significant correlations under HNT conditions. Solid lines indicate positive, dashed line negative correlations.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588888&req=5

Figure 6: Significant (  p<0.05) correlations of the polyamines Put, Spd and Spm with 75 other metabolites. (A) Significant correlations under control conditions. (B) Significant correlations under HNT conditions. Solid lines indicate positive, dashed line negative correlations.
Mentions: Since the content of all three polyamines was significantly correlated with the HNT sensitivity of the cultivars, we wanted to further elucidate the connection of this pathway with the central metabolism. We therefore performed a Spearman’s rank order correlation analysis of polyamine pool sizes with those of all other measured metabolites. Significant correlations differed widely between control and HNT conditions, with six and 19 correlations, respectively (Fig. 6). While Spd was positively correlated with Put and Spm under both conditions, Put was additionally correlated with Spm under HNT conditions. In addition, only the correlations of Spm with glyceric acid 2-P and glyceric acid 3-P were observed under both conditions and these metabolites were not correlated with HNT sensitivity. However, eight correlations of polyamines with amino acids or organic acids, whose levels were correlated with HNT sensitivity, were specific to HNT conditions. Further, a negative correlation between Spm and myo-inositol pool sizes was observed under HNT, where also a negative correlation of this sugar alcohol with HNT sensitivity was observed.

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.