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High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.


(A) Put, (B) Spd and (C) Spm content of leaves 48 DAS under control (grey columns) and 23 d (48 DAS) of HNT conditions (black columns) in 12 rice cultivars. Each value represents the average of five replicates from one experiment per condition. Cultivars are sorted from tolerant to sensitive (left to right).
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Figure 5: (A) Put, (B) Spd and (C) Spm content of leaves 48 DAS under control (grey columns) and 23 d (48 DAS) of HNT conditions (black columns) in 12 rice cultivars. Each value represents the average of five replicates from one experiment per condition. Cultivars are sorted from tolerant to sensitive (left to right).

Mentions: Under control conditions Put levels were highly cultivar dependent with the lowest levels in the sensitive cultivar M202 (Fig. 5A). In agreement with the GC-MS analysis, Put levels were increased in sensitive cultivars under HNT conditions, with a significant increase for M202 (Fig. 5A). A similar pattern was detected for Spd with a significant increase in the sensitive cultivar IR62266-42-6-2 and a significant decrease in the tolerant cultivar Taipei 309 (Fig. 5B). Spm levels were significantly increased in the sensitive cultivars DR2 and IR62266-42-6-2 and in the intermediate cultivars IR64 and IRRI123 (Fig. 5C). Correlation analyses of polyamine levels with sensitivity rank revealed positive correlations for Put (r=0.351, p=0.0076), Spd (r=0.392, p=0.0027) and Spm (r=0.352, p=0.0075) under HNT but not under control conditions.


High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

(A) Put, (B) Spd and (C) Spm content of leaves 48 DAS under control (grey columns) and 23 d (48 DAS) of HNT conditions (black columns) in 12 rice cultivars. Each value represents the average of five replicates from one experiment per condition. Cultivars are sorted from tolerant to sensitive (left to right).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588888&req=5

Figure 5: (A) Put, (B) Spd and (C) Spm content of leaves 48 DAS under control (grey columns) and 23 d (48 DAS) of HNT conditions (black columns) in 12 rice cultivars. Each value represents the average of five replicates from one experiment per condition. Cultivars are sorted from tolerant to sensitive (left to right).
Mentions: Under control conditions Put levels were highly cultivar dependent with the lowest levels in the sensitive cultivar M202 (Fig. 5A). In agreement with the GC-MS analysis, Put levels were increased in sensitive cultivars under HNT conditions, with a significant increase for M202 (Fig. 5A). A similar pattern was detected for Spd with a significant increase in the sensitive cultivar IR62266-42-6-2 and a significant decrease in the tolerant cultivar Taipei 309 (Fig. 5B). Spm levels were significantly increased in the sensitive cultivars DR2 and IR62266-42-6-2 and in the intermediate cultivars IR64 and IRRI123 (Fig. 5C). Correlation analyses of polyamine levels with sensitivity rank revealed positive correlations for Put (r=0.351, p=0.0076), Spd (r=0.392, p=0.0027) and Spm (r=0.352, p=0.0075) under HNT but not under control conditions.

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.