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High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.


(A) Hierarchical cluster analysis (HCA) with Pearson correlation on 75 metabolites measured by GC-MS 48 DAS under control (left) and 23 d (48 DAS) of HNT (right) conditions in 12 rice cultivars indicated at the top of the figure. Metabolites with high intensities compared to the median are coloured in red, metabolites with low intensities in blue. Gray indicates a missing value. Cultivars are sorted from tolerant to sensitive (left to right). All metabolite data can be found in Supplementary Table S2. (B) Normalized responses of selected metabolites representing the clusters CI−V are shown in panel.
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Figure 2: (A) Hierarchical cluster analysis (HCA) with Pearson correlation on 75 metabolites measured by GC-MS 48 DAS under control (left) and 23 d (48 DAS) of HNT (right) conditions in 12 rice cultivars indicated at the top of the figure. Metabolites with high intensities compared to the median are coloured in red, metabolites with low intensities in blue. Gray indicates a missing value. Cultivars are sorted from tolerant to sensitive (left to right). All metabolite data can be found in Supplementary Table S2. (B) Normalized responses of selected metabolites representing the clusters CI−V are shown in panel.

Mentions: HCA was performed using Pearson correlation and resulted in five main clusters (CI−V) of metabolites with similar response patterns (Fig. 2A). In agreement with the results of the PCA, intermediate and sensitive cultivars showed the strongest differences in metabolite content under HNT conditions. M202 showed the highest number of metabolites with increased pool sizes upon exposure to HNT conditions. The different clusters represent different response patterns of the respective metabolites to HNT with almost no changes in CI, a minor increase of the metabolite levels in CII, a large increase in CIII in the three most sensitive cultivars and an increase in most cultivars in CIV. Pool sizes of some metabolites were only increased in sensitive cultivars, in particular those of nine amino acids (threonine is shown as an example for CIII in Fig. 2B). Also levels of the polyamine putrescine (Put), succinic and 2-oxo-glutaric acid in CIV, TCA cycle intermediates like malic acid, as well as organic acids like aspartic, pyroglutamic, erythronic, nicotinic, threonic, glucoronic and galacturonic acid (CIII) were increased in sensitive cultivars. Most of the changes in CIII and CIV occurred to a lesser extent also in intermediate cultivars, whereas the tolerant cultivars were mostly unaffected by HNT.


High night temperature strongly impacts TCA cycle, amino acid and polyamine biosynthetic pathways in rice in a sensitivity-dependent manner.

Glaubitz U, Erban A, Kopka J, Hincha DK, Zuther E - J. Exp. Bot. (2015)

(A) Hierarchical cluster analysis (HCA) with Pearson correlation on 75 metabolites measured by GC-MS 48 DAS under control (left) and 23 d (48 DAS) of HNT (right) conditions in 12 rice cultivars indicated at the top of the figure. Metabolites with high intensities compared to the median are coloured in red, metabolites with low intensities in blue. Gray indicates a missing value. Cultivars are sorted from tolerant to sensitive (left to right). All metabolite data can be found in Supplementary Table S2. (B) Normalized responses of selected metabolites representing the clusters CI−V are shown in panel.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588888&req=5

Figure 2: (A) Hierarchical cluster analysis (HCA) with Pearson correlation on 75 metabolites measured by GC-MS 48 DAS under control (left) and 23 d (48 DAS) of HNT (right) conditions in 12 rice cultivars indicated at the top of the figure. Metabolites with high intensities compared to the median are coloured in red, metabolites with low intensities in blue. Gray indicates a missing value. Cultivars are sorted from tolerant to sensitive (left to right). All metabolite data can be found in Supplementary Table S2. (B) Normalized responses of selected metabolites representing the clusters CI−V are shown in panel.
Mentions: HCA was performed using Pearson correlation and resulted in five main clusters (CI−V) of metabolites with similar response patterns (Fig. 2A). In agreement with the results of the PCA, intermediate and sensitive cultivars showed the strongest differences in metabolite content under HNT conditions. M202 showed the highest number of metabolites with increased pool sizes upon exposure to HNT conditions. The different clusters represent different response patterns of the respective metabolites to HNT with almost no changes in CI, a minor increase of the metabolite levels in CII, a large increase in CIII in the three most sensitive cultivars and an increase in most cultivars in CIV. Pool sizes of some metabolites were only increased in sensitive cultivars, in particular those of nine amino acids (threonine is shown as an example for CIII in Fig. 2B). Also levels of the polyamine putrescine (Put), succinic and 2-oxo-glutaric acid in CIV, TCA cycle intermediates like malic acid, as well as organic acids like aspartic, pyroglutamic, erythronic, nicotinic, threonic, glucoronic and galacturonic acid (CIII) were increased in sensitive cultivars. Most of the changes in CIII and CIV occurred to a lesser extent also in intermediate cultivars, whereas the tolerant cultivars were mostly unaffected by HNT.

Bottom Line: Increased expression levels of ADC2 and ODC1, genes encoding enzymes catalysing the first committed steps of putrescine biosynthesis, were restricted to sensitive cultivars under HNT.Additionally, transcript levels of eight polyamine biosynthesis genes were correlated with HNT sensitivity.Responses to HNT in the vegetative stage result in distinct differences between differently responding cultivars with a dysregulation of central metabolism and an increase of polyamine biosynthesis restricted to sensitive cultivars under HNT conditions and a pre-adaptation of tolerant cultivars already under control conditions with higher levels of potentially protective compatible solutes.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.

No MeSH data available.