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A role for seed storage proteins in Arabidopsis seed longevity.

Nguyen TP, Cueff G, Hegedus DD, Rajjou L, Bentsink L - J. Exp. Bot. (2015)

Bottom Line: Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing.Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity.Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds.

View Article: PubMed Central - PubMed

Affiliation: Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands.

No MeSH data available.


The effect of seed storage proteins (SSPs) on seed longevity and seed dormancy.(A) Seed longevity presented as germination (%) of different SSP knock-out lines was measured after 10 d of artificial ageing. The lines include the wild-type Col, as well as single (crua, aBC; crub, AbC and cruc, ABc), double (crub cruc, Abc; crua cruc, aBc and crua crub, abC) and triple (crua crub cruc, abc) knock-out lines of cruciferins, and an RNAi napin line that is depleted of napins. (B) Seed dormancy presented as days of seed dry storage required to reach 50% germination (DSDS50) of Col and different SSP knock-out lines.
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Figure 5: The effect of seed storage proteins (SSPs) on seed longevity and seed dormancy.(A) Seed longevity presented as germination (%) of different SSP knock-out lines was measured after 10 d of artificial ageing. The lines include the wild-type Col, as well as single (crua, aBC; crub, AbC and cruc, ABc), double (crub cruc, Abc; crua cruc, aBc and crua crub, abC) and triple (crua crub cruc, abc) knock-out lines of cruciferins, and an RNAi napin line that is depleted of napins. (B) Seed dormancy presented as days of seed dry storage required to reach 50% germination (DSDS50) of Col and different SSP knock-out lines.

Mentions: AT2S3, one of the five 2S albumin or napin isoforms (Krebbers et al., 1988; Van der Klei et al., 1993), was more abundant in aged NILGAAS5 (the most sensitive genotype to ageing) (Table 1B). The AT2S3 protein spot ID1505 might be a degradation product due to its altered MW and pI (Table 1B). To examine if napins could affect seed longevity, a napin RNAi line was analysed for seed longevity (Withana-Gamage et al., 2013) (Supplementary Table S3). The line, which is depleted of napins and has a reduced protein content mainly in the endosperm, was more sensitive to ageing than wild-type Col (Fig. 5).


A role for seed storage proteins in Arabidopsis seed longevity.

Nguyen TP, Cueff G, Hegedus DD, Rajjou L, Bentsink L - J. Exp. Bot. (2015)

The effect of seed storage proteins (SSPs) on seed longevity and seed dormancy.(A) Seed longevity presented as germination (%) of different SSP knock-out lines was measured after 10 d of artificial ageing. The lines include the wild-type Col, as well as single (crua, aBC; crub, AbC and cruc, ABc), double (crub cruc, Abc; crua cruc, aBc and crua crub, abC) and triple (crua crub cruc, abc) knock-out lines of cruciferins, and an RNAi napin line that is depleted of napins. (B) Seed dormancy presented as days of seed dry storage required to reach 50% germination (DSDS50) of Col and different SSP knock-out lines.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588887&req=5

Figure 5: The effect of seed storage proteins (SSPs) on seed longevity and seed dormancy.(A) Seed longevity presented as germination (%) of different SSP knock-out lines was measured after 10 d of artificial ageing. The lines include the wild-type Col, as well as single (crua, aBC; crub, AbC and cruc, ABc), double (crub cruc, Abc; crua cruc, aBc and crua crub, abC) and triple (crua crub cruc, abc) knock-out lines of cruciferins, and an RNAi napin line that is depleted of napins. (B) Seed dormancy presented as days of seed dry storage required to reach 50% germination (DSDS50) of Col and different SSP knock-out lines.
Mentions: AT2S3, one of the five 2S albumin or napin isoforms (Krebbers et al., 1988; Van der Klei et al., 1993), was more abundant in aged NILGAAS5 (the most sensitive genotype to ageing) (Table 1B). The AT2S3 protein spot ID1505 might be a degradation product due to its altered MW and pI (Table 1B). To examine if napins could affect seed longevity, a napin RNAi line was analysed for seed longevity (Withana-Gamage et al., 2013) (Supplementary Table S3). The line, which is depleted of napins and has a reduced protein content mainly in the endosperm, was more sensitive to ageing than wild-type Col (Fig. 5).

Bottom Line: Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing.Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity.Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds.

View Article: PubMed Central - PubMed

Affiliation: Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands.

No MeSH data available.