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A role for seed storage proteins in Arabidopsis seed longevity.

Nguyen TP, Cueff G, Hegedus DD, Rajjou L, Bentsink L - J. Exp. Bot. (2015)

Bottom Line: Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing.Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity.Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds.

View Article: PubMed Central - PubMed

Affiliation: Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands.

No MeSH data available.


Seed germination after seed dry storage. The germination (%) of Ler and the near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5 were analysed for after-ripened seeds (open bars) and naturally-aged seeds after four years of storage (filled bars). Averages of four biological replicates with standard errors are presented. The asterisks indicate significant differences between aged NILs and Ler (P<0.05).
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Figure 1: Seed germination after seed dry storage. The germination (%) of Ler and the near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5 were analysed for after-ripened seeds (open bars) and naturally-aged seeds after four years of storage (filled bars). Averages of four biological replicates with standard errors are presented. The asterisks indicate significant differences between aged NILs and Ler (P<0.05).

Mentions: The four A. thaliana genotypes, namely Ler, NILGAAS1(-Cape Verde Islands (Cvi)), NILGAAS2(-Antwerp (An-1)) and NILGAAS5 (-Shakdara (Sha)), were originally developed as NILDOG2, NILDOG22 and NILDOG1, respectively (Bentsink et al., 2010; Nguyen et al., 2012). Those genotypes were grown in a randomized complete block design with replicates in soil as described in Bentsink et al. (2010). Seeds of four plants per replicate were bulked. Proteome analyses were conducted for the four genotypes at two physiological stages, fully AR and 4-year-old (aged) seeds. Fully AR seeds are competent to germinate 100% while aged seeds have germination ability reduced; in this study germination phenotype of those seeds were assessed (Fig. 1).


A role for seed storage proteins in Arabidopsis seed longevity.

Nguyen TP, Cueff G, Hegedus DD, Rajjou L, Bentsink L - J. Exp. Bot. (2015)

Seed germination after seed dry storage. The germination (%) of Ler and the near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5 were analysed for after-ripened seeds (open bars) and naturally-aged seeds after four years of storage (filled bars). Averages of four biological replicates with standard errors are presented. The asterisks indicate significant differences between aged NILs and Ler (P<0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588887&req=5

Figure 1: Seed germination after seed dry storage. The germination (%) of Ler and the near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5 were analysed for after-ripened seeds (open bars) and naturally-aged seeds after four years of storage (filled bars). Averages of four biological replicates with standard errors are presented. The asterisks indicate significant differences between aged NILs and Ler (P<0.05).
Mentions: The four A. thaliana genotypes, namely Ler, NILGAAS1(-Cape Verde Islands (Cvi)), NILGAAS2(-Antwerp (An-1)) and NILGAAS5 (-Shakdara (Sha)), were originally developed as NILDOG2, NILDOG22 and NILDOG1, respectively (Bentsink et al., 2010; Nguyen et al., 2012). Those genotypes were grown in a randomized complete block design with replicates in soil as described in Bentsink et al. (2010). Seeds of four plants per replicate were bulked. Proteome analyses were conducted for the four genotypes at two physiological stages, fully AR and 4-year-old (aged) seeds. Fully AR seeds are competent to germinate 100% while aged seeds have germination ability reduced; in this study germination phenotype of those seeds were assessed (Fig. 1).

Bottom Line: Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing.Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity.Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds.

View Article: PubMed Central - PubMed

Affiliation: Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands.

No MeSH data available.