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A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.


ABA-induced stomatal closure (A) and ABA-inhibited light-induced stomatal opening (B) in the wild-type Col, the pyr1 pyl1 pyl2 pyl4 quadruple mutant (quadruple), and two mutant alleles of the ABAR/CHLH gene (cch and rtl1). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment.
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Figure 5: ABA-induced stomatal closure (A) and ABA-inhibited light-induced stomatal opening (B) in the wild-type Col, the pyr1 pyl1 pyl2 pyl4 quadruple mutant (quadruple), and two mutant alleles of the ABAR/CHLH gene (cch and rtl1). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment.

Mentions: To assess a possible mechanism by which ABAR and OST1 interact in ABA signalling, ABA-induced ROS and NO production in guard cells was measured in the ABAR gene mutants, using the pyr1 pyl1 pyl2 pyl4 quadruple mutant of the PYR/PYL/RCAR gene family, encoding the cytosolic receptors for ABA upstream of OST1 as a control. It was first confirmed that the two mutant alleles, cch and rtl1, of the ABAR gene show both strong ABA-insensitive phenotypes in ABA-induced stomatal closure and inhibition of light-induced stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA-insensitive phenotypes only in ABA-induced stomatal closure but not in ABA inhibition of light-induced stomatal opening (Fig. 5). The pyr1 pyl1 pyl2 pyl4 quadruple mutant showed slight ABA-insensitive phenotypes in ABA inhibition of stomatal opening with 15 μM (±)ABA application, and the phenotypic intensity was significantly weaker than that of cch or rtl1, while in a higher ABA concentration [30 μM (±)ABA], the pyr1 pyl1 pyl2 pyl4 quadruple mutant showed a wild-type ABA response in light-induced stomatal opening (Fig. 5), which is largely consistent with previous observations (Yin et al., 2013).


A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

ABA-induced stomatal closure (A) and ABA-inhibited light-induced stomatal opening (B) in the wild-type Col, the pyr1 pyl1 pyl2 pyl4 quadruple mutant (quadruple), and two mutant alleles of the ABAR/CHLH gene (cch and rtl1). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: ABA-induced stomatal closure (A) and ABA-inhibited light-induced stomatal opening (B) in the wild-type Col, the pyr1 pyl1 pyl2 pyl4 quadruple mutant (quadruple), and two mutant alleles of the ABAR/CHLH gene (cch and rtl1). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment.
Mentions: To assess a possible mechanism by which ABAR and OST1 interact in ABA signalling, ABA-induced ROS and NO production in guard cells was measured in the ABAR gene mutants, using the pyr1 pyl1 pyl2 pyl4 quadruple mutant of the PYR/PYL/RCAR gene family, encoding the cytosolic receptors for ABA upstream of OST1 as a control. It was first confirmed that the two mutant alleles, cch and rtl1, of the ABAR gene show both strong ABA-insensitive phenotypes in ABA-induced stomatal closure and inhibition of light-induced stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA-insensitive phenotypes only in ABA-induced stomatal closure but not in ABA inhibition of light-induced stomatal opening (Fig. 5). The pyr1 pyl1 pyl2 pyl4 quadruple mutant showed slight ABA-insensitive phenotypes in ABA inhibition of stomatal opening with 15 μM (±)ABA application, and the phenotypic intensity was significantly weaker than that of cch or rtl1, while in a higher ABA concentration [30 μM (±)ABA], the pyr1 pyl1 pyl2 pyl4 quadruple mutant showed a wild-type ABA response in light-induced stomatal opening (Fig. 5), which is largely consistent with previous observations (Yin et al., 2013).

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.