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A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.


Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: OST1 over-expression suppresses ABA-insensitive phenotypes of the cch mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, cch mutant, OST1 over-expression line under Col background (OST1OE-1), and OST1 over-expression line under cch background (OST1OE-1/cch). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B). Status of the detached leaves of the Col, cch, OST1OE-1, and OST1OE-1/cch, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings of the Col, cch, OST1OE-1, and OST1OE-1/cch. Plants were well watered for 5 d then drought-stressed by withholding water for 14 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
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Figure 4: Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: OST1 over-expression suppresses ABA-insensitive phenotypes of the cch mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, cch mutant, OST1 over-expression line under Col background (OST1OE-1), and OST1 over-expression line under cch background (OST1OE-1/cch). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B). Status of the detached leaves of the Col, cch, OST1OE-1, and OST1OE-1/cch, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings of the Col, cch, OST1OE-1, and OST1OE-1/cch. Plants were well watered for 5 d then drought-stressed by withholding water for 14 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.

Mentions: To further investigate functional interaction between ABAR and OST1, OST1-over-expression lines were generated in which the OST1 protein was Myc-tagged (Supplementary Fig. S3A). The OST1-transgenic lines displayed ABA-hypersensitive response in stomatal movement as previously reported (Acharya et al., 2013), and the intensities of the ABA-hypersensitive phenotypes were positively correlated with the OST1-expression levels (Supplementary Fig. S3B). The OST1 over-expression line (OST1OE-1) was crossed with the cch mutant to create an OST1 over-expression line under the cch mutant background (OST1OE-1/cch). This OST1OE-1/cch line showed ABA-hypersensitive phenotypes in ABA-induced stomatal closure and ABA-inhibited stomatal opening like the OST1 over-expression line, which suppresses ABA-insensitive phenotypes of the cch mutant (Fig. 4A). The OST1OE-1 showed dehydration tolerance in contrast to cch that is dehydration hypersensitive, and the OST1OE-1/cch line showed dehydration tolerance like the OST1OE-1 line in the assays in both detached leaves and whole plants (Fig. 4B–D), which is consistent with the data from the assays of stomatal movement in response to ABA (Fig. 4A).


A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: OST1 over-expression suppresses ABA-insensitive phenotypes of the cch mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, cch mutant, OST1 over-expression line under Col background (OST1OE-1), and OST1 over-expression line under cch background (OST1OE-1/cch). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B). Status of the detached leaves of the Col, cch, OST1OE-1, and OST1OE-1/cch, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings of the Col, cch, OST1OE-1, and OST1OE-1/cch. Plants were well watered for 5 d then drought-stressed by withholding water for 14 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
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Figure 4: Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: OST1 over-expression suppresses ABA-insensitive phenotypes of the cch mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, cch mutant, OST1 over-expression line under Col background (OST1OE-1), and OST1 over-expression line under cch background (OST1OE-1/cch). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B). Status of the detached leaves of the Col, cch, OST1OE-1, and OST1OE-1/cch, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings of the Col, cch, OST1OE-1, and OST1OE-1/cch. Plants were well watered for 5 d then drought-stressed by withholding water for 14 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
Mentions: To further investigate functional interaction between ABAR and OST1, OST1-over-expression lines were generated in which the OST1 protein was Myc-tagged (Supplementary Fig. S3A). The OST1-transgenic lines displayed ABA-hypersensitive response in stomatal movement as previously reported (Acharya et al., 2013), and the intensities of the ABA-hypersensitive phenotypes were positively correlated with the OST1-expression levels (Supplementary Fig. S3B). The OST1 over-expression line (OST1OE-1) was crossed with the cch mutant to create an OST1 over-expression line under the cch mutant background (OST1OE-1/cch). This OST1OE-1/cch line showed ABA-hypersensitive phenotypes in ABA-induced stomatal closure and ABA-inhibited stomatal opening like the OST1 over-expression line, which suppresses ABA-insensitive phenotypes of the cch mutant (Fig. 4A). The OST1OE-1 showed dehydration tolerance in contrast to cch that is dehydration hypersensitive, and the OST1OE-1/cch line showed dehydration tolerance like the OST1OE-1 line in the assays in both detached leaves and whole plants (Fig. 4B–D), which is consistent with the data from the assays of stomatal movement in response to ABA (Fig. 4A).

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.