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A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.


Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: ABAR over-expression does not significantly affect ABA-insensitive phenotypes of the srk2e mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, srk2e sigle mutant, ABAR631–1381 over-expression line under Col backgroud (ABAR631–1381OE), and ABAR631–1381 over-expression line under srk2e backgroud (ABAR631–1381OE/srk2e). ABAR631–1381 is a truncated form of ABAR (aa 631–1381). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B) Status of the detached leaves of the Col, srk2e, ABAR631–1381OE, and ABAR631–1381OE/srk2e, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings for the Col, srk2e, ABAR631–-1381OE, and ABAR631–1381OE/srk2e. Plants were well watered for 5 d then drought-stressed by withholding water for 17 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
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Figure 3: Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: ABAR over-expression does not significantly affect ABA-insensitive phenotypes of the srk2e mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, srk2e sigle mutant, ABAR631–1381 over-expression line under Col backgroud (ABAR631–1381OE), and ABAR631–1381 over-expression line under srk2e backgroud (ABAR631–1381OE/srk2e). ABAR631–1381 is a truncated form of ABAR (aa 631–1381). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B) Status of the detached leaves of the Col, srk2e, ABAR631–1381OE, and ABAR631–1381OE/srk2e, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings for the Col, srk2e, ABAR631–-1381OE, and ABAR631–1381OE/srk2e. Plants were well watered for 5 d then drought-stressed by withholding water for 17 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.

Mentions: It has been known that the over-expression of either the C-terminal half of ABAR (aa 631–1381) in whole Col plants (ABAR631–1381OE, Wu et al., 2009) or the full-length ABAR specifically in guard cells (Tsuzuki et al., 2013) confers ABA hypersensitivity in ABA-mediated stomatal response. ABAR631–1381- over-expression lines were created under the srk2e mutant background by crossing (ABAR631–1381OE/srk2e, Supplementary Fig. S2), which did not suppress the srk2e mutant phenotype, but showed an ABA-insensitive phenotype, like the srk2e background, in ABA-induced stomatal closure and ABA-inhibited stomatal opening (Fig. 3A). In addition, whereas over-expression of ABAR631–1381 in the Col background improved dehydration tolerance, over-expression of the same truncated ABAR under srk2e mutant did not affect the dehydration overly sensitive phenotypes of the srk2e mutant (Fig. 3B–D), which is consistent with the data from the investigation on stomatal movement in response to ABA (Fig. 3A).


A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

Liang S, Lu K, Wu Z, Jiang SC, Yu YT, Bi C, Xin Q, Wang XF, Zhang DP - J. Exp. Bot. (2015)

Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: ABAR over-expression does not significantly affect ABA-insensitive phenotypes of the srk2e mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, srk2e sigle mutant, ABAR631–1381 over-expression line under Col backgroud (ABAR631–1381OE), and ABAR631–1381 over-expression line under srk2e backgroud (ABAR631–1381OE/srk2e). ABAR631–1381 is a truncated form of ABAR (aa 631–1381). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B) Status of the detached leaves of the Col, srk2e, ABAR631–1381OE, and ABAR631–1381OE/srk2e, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings for the Col, srk2e, ABAR631–-1381OE, and ABAR631–1381OE/srk2e. Plants were well watered for 5 d then drought-stressed by withholding water for 17 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
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Figure 3: Genetic interaction between ABAR/CHLH and OST1/SnRK2.6/SRK2E: ABAR over-expression does not significantly affect ABA-insensitive phenotypes of the srk2e mutant in stomatal movement. (A) ABA-induced stomatal closure (top) and inhibition of stomatal opening (bottom) in wild-type Col, srk2e sigle mutant, ABAR631–1381 over-expression line under Col backgroud (ABAR631–1381OE), and ABAR631–1381 over-expression line under srk2e backgroud (ABAR631–1381OE/srk2e). ABAR631–1381 is a truncated form of ABAR (aa 631–1381). Values are means ±SE from three independent experiments, and different letters indicate significant differences at P<0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. n≥60 apertures per experiment. (B) Status of the detached leaves of the Col, srk2e, ABAR631–1381OE, and ABAR631–1381OE/srk2e, which were subjected to a 6-h period water loss assay. (C) Water loss rates during a 6-h period from the detached leaves of the different genotypes described in (B). Values are means ±SE from three independent experiments. *P<0.05 (Duncan’s multiple range test) when comparing values within the same time point. (D) Water loss assays with young seedlings for the Col, srk2e, ABAR631–-1381OE, and ABAR631–1381OE/srk2e. Plants were well watered for 5 d then drought-stressed by withholding water for 17 d (bottom). Top panel shows the well watered control plants. The entire experiment was replicated three times with similar results.
Mentions: It has been known that the over-expression of either the C-terminal half of ABAR (aa 631–1381) in whole Col plants (ABAR631–1381OE, Wu et al., 2009) or the full-length ABAR specifically in guard cells (Tsuzuki et al., 2013) confers ABA hypersensitivity in ABA-mediated stomatal response. ABAR631–1381- over-expression lines were created under the srk2e mutant background by crossing (ABAR631–1381OE/srk2e, Supplementary Fig. S2), which did not suppress the srk2e mutant phenotype, but showed an ABA-insensitive phenotype, like the srk2e background, in ABA-induced stomatal closure and ABA-inhibited stomatal opening (Fig. 3A). In addition, whereas over-expression of ABAR631–1381 in the Col background improved dehydration tolerance, over-expression of the same truncated ABAR under srk2e mutant did not affect the dehydration overly sensitive phenotypes of the srk2e mutant (Fig. 3B–D), which is consistent with the data from the investigation on stomatal movement in response to ABA (Fig. 3A).

Bottom Line: Neither mutation nor over-expression of the ABAR gene affects significantly ABA-insensitive phenotypes of stomatal movement in the OST1 knockout mutant allele srk2e.These findings suggest that ABAR shares, at least in part, downstream signalling components with PYR/PYL/RCAR receptors for ABA in guard cells; though cch and rtl1 show strong ABA-insensitive phenotypes in both ABA-induced stomatal closure and inhibition of stomatal opening, while the pyr1 pyl1 pyl2 pyl4 quadruple mutant shows strong ABA insensitivity only in ABA-induced stomatal closure.These data establish a link between ABAR/CHLH and SnRK2.6/OST1 in guard cell signalling in response to ABA.

View Article: PubMed Central - PubMed

Affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

No MeSH data available.