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Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Genes strongly regulated by N deprivation. The genes most up- or downregulated after 72h of nitrate deprivation are shown for the processes listed at the top of the graph. The ratios were log2 transformed. Numbers indicate Phatr2 gene IDs.
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Figure 4: Genes strongly regulated by N deprivation. The genes most up- or downregulated after 72h of nitrate deprivation are shown for the processes listed at the top of the graph. The ratios were log2 transformed. Numbers indicate Phatr2 gene IDs.

Mentions: Strong transcriptional responses were observed for genes encoding proteins involved in processes such as photosynthesis, central carbon metabolism, lipid metabolism, nitrogen metabolism and transport, and amino acid metabolism, as discussed below. The responses of the strongest regulated genes within these categories 72h after N deprivation are shown in Fig. 4. In order to verify the results of microarray analysis, qRT-PCR was performed on five selected genes involved in photosynthesis, and N and carbon metabolism, respectively, that were differentially regulated at 48 and 72h in the microarray analysis. The qRT-PCR results correlated well with the microarray analysis (Supplementary Fig. S2, available at JXB online).


Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Genes strongly regulated by N deprivation. The genes most up- or downregulated after 72h of nitrate deprivation are shown for the processes listed at the top of the graph. The ratios were log2 transformed. Numbers indicate Phatr2 gene IDs.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588885&req=5

Figure 4: Genes strongly regulated by N deprivation. The genes most up- or downregulated after 72h of nitrate deprivation are shown for the processes listed at the top of the graph. The ratios were log2 transformed. Numbers indicate Phatr2 gene IDs.
Mentions: Strong transcriptional responses were observed for genes encoding proteins involved in processes such as photosynthesis, central carbon metabolism, lipid metabolism, nitrogen metabolism and transport, and amino acid metabolism, as discussed below. The responses of the strongest regulated genes within these categories 72h after N deprivation are shown in Fig. 4. In order to verify the results of microarray analysis, qRT-PCR was performed on five selected genes involved in photosynthesis, and N and carbon metabolism, respectively, that were differentially regulated at 48 and 72h in the microarray analysis. The qRT-PCR results correlated well with the microarray analysis (Supplementary Fig. S2, available at JXB online).

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus