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Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


GO analysis of significantly regulated genes after 72h of nitrate deprivation. The dataset was divided into up- and downregulated genes and analysed for process GO terms. The 15 most frequent GO terms are listed, and the rest were combined into ‘others’. The number in the ‘others’ section indicates the number of hits within this category. The total number of GO term hits is listed below the graphs.
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Figure 3: GO analysis of significantly regulated genes after 72h of nitrate deprivation. The dataset was divided into up- and downregulated genes and analysed for process GO terms. The 15 most frequent GO terms are listed, and the rest were combined into ‘others’. The number in the ‘others’ section indicates the number of hits within this category. The total number of GO term hits is listed below the graphs.

Mentions: GO analysis was performed on the dataset. As the P. tricornutum genome is still poorly annotated, GO terms are assigned to a limited number of genes. The GO analysis still provided an overview of the processes most affected by N deprivation. The most enriched GO terms were similar at 48h (Supplementary Fig. S1, available at JXB online) and 72h (Fig. 3); however, there were large differences between GO terms enriched in up- and downregulated genes. The most frequent GO term among the downregulated genes was protein biosynthesis. Other GO terms related to ribosomal assembly and translation were also enriched among downregulated genes, indicating reduced protein biosynthesis. Furthermore, photosynthesis light harvesting was the fourth most used GO term among the downregulated genes, indicating downsizing of the light-harvesting apparatus. In contrast, the upregulated genes were enriched in GO terms related to signal transduction and ubiquitination.


Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

GO analysis of significantly regulated genes after 72h of nitrate deprivation. The dataset was divided into up- and downregulated genes and analysed for process GO terms. The 15 most frequent GO terms are listed, and the rest were combined into ‘others’. The number in the ‘others’ section indicates the number of hits within this category. The total number of GO term hits is listed below the graphs.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588885&req=5

Figure 3: GO analysis of significantly regulated genes after 72h of nitrate deprivation. The dataset was divided into up- and downregulated genes and analysed for process GO terms. The 15 most frequent GO terms are listed, and the rest were combined into ‘others’. The number in the ‘others’ section indicates the number of hits within this category. The total number of GO term hits is listed below the graphs.
Mentions: GO analysis was performed on the dataset. As the P. tricornutum genome is still poorly annotated, GO terms are assigned to a limited number of genes. The GO analysis still provided an overview of the processes most affected by N deprivation. The most enriched GO terms were similar at 48h (Supplementary Fig. S1, available at JXB online) and 72h (Fig. 3); however, there were large differences between GO terms enriched in up- and downregulated genes. The most frequent GO term among the downregulated genes was protein biosynthesis. Other GO terms related to ribosomal assembly and translation were also enriched among downregulated genes, indicating reduced protein biosynthesis. Furthermore, photosynthesis light harvesting was the fourth most used GO term among the downregulated genes, indicating downsizing of the light-harvesting apparatus. In contrast, the upregulated genes were enriched in GO terms related to signal transduction and ubiquitination.

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.